This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Vascular Hyperpermeability: Methods and Protocols is a valuable resource that will aid novice researchers with creating new and affordable research projects, and for expert researchers to initiate new strategies and collaborations in their ongoing programs.
Determination of Solute Permeability of Microvascular Endothelial Cell Monolayers In Vitro.- Evaluation of Vascular Permeability in Inflamed Vessels of the Cremaster Muscle in Live Mice.- Lymphatic Vascular Permeability Determined from Direct Measurements of Solute Flux.- Evaluation of Mesenteric Microvascular Hyperpermeability Following Hemorrhagic Shock using Intravital Microscopy.- Determination of Endothelial Barrier Resistance by Electric Cell-Substrate Impedance Sensing (ECIS) System.- Time-Lapse Observation of Cell Dynamics during Angiogenesis Using the Rat Mesentery Culture Model.- Evaluation of Barrier Integrity using a Two-Layered Microfluidic Device Mimicking the Blood-Brain Barrier.- Intravital Imaging of Leukocyte-Endothelial Interaction in Hindlimb Ischemia/Reperfusion Injury by Intravital Multiphoton Microscopy.- Studying Angiogenesis using Matrigel In Vitro and In Vivo.- Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy.- Imaging and Analysis of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells.- Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs).- Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification.- Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay.- Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model.- Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells.- An In Vitro Bilayer Model of Human Primary Retinal Pigment Epithelial and Choroid Endothelial Cells for Permeability Studies.- Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro.- Determination of Tight Junction Integrity in Brain Endothelial Cells Based on Tight Junction Protein Expression.- Evaluation of Glycolysis and Mitochondrial Function in Endothelial Cells Using the Seahorse Analyzer.- Evaluation of Tight Junction Integrity in Brain Endothelial Cells using Confocal Microscopy.
Inhaltsverzeichnis
Preface...
Table of Contents...
Contributing Authors...
1.Determination of Solute Permeability of Microvascular Endothelial Cell Monolayers In Vitro
Jerome W. Breslin and Sarah Y. Yuan
2.Evaluation of Vascular Permeability in Inflamed Vessels of the Cremaster Muscle in Live Mice
Soi Jeong, Young Ho Choe, Young Min Kim, and Young-Min Hyun
3.Lymphatic Vascular Permeability Determined from Direct Measurements of Solute Flux
Melanie Jannaway and Joshua P. Scallan
4.Evaluation of Mesenteric Microvascular Hyperpermeability Following Hemorrhagic Shock using Intravital Microscopy
Taylor R. Williams and Ed W. Childs
5.Determination of Endothelial Barrier Resistance by Electric Cell-Substrate Impedance Sensing (ECIS) System
Hemant Giri and Madhulika Dixit
6.Time-Lapse Observation of Cell Dynamics during Angiogenesis Using the Rat Mesentery Culture Model
Arinola O. Lampejo, Nicholas A. Hodges, Maximillian Rozenblum, and Walter L. Murfee
7.Evaluation of Barrier Integrity using a Two-Layered Microfluidic Device Mimicking the Blood-Brain Barrier
Hossam Kadry and Luca Cucullo
8.Intravital Imaging of Leukocyte-Endothelial Interaction in Hindlimb Ischemia/Reperfusion Injury by Intravital Multiphoton Microscopy
Johannes Zeller, Karlheinz Peter, and Steffen U. Eisenhardt
9.Studying Angiogenesis using Matrigel In Vitro and In Vivo
Anantha K. Kanugula, Ravi K. Adapala, Brianna D. Guarino, Neha Bhavnani, Harshitha Dudipala, Sailaja Paruchuri, and Charles K. Thodeti
10.Determination of Blood-Brain Barrier Hyperpermeability Using Intravital Microscopy
O'lisa Yaa Waithe, Chinchusha Anasooya Shaji, Ed W. Childs, and Binu Tharakan
11.Imaging and Analysis of the Dynamics of Filamentous Actin Structures in Live Endothelial Cells
Jerome W. Breslin and Zeinab Y. Motawe
12.Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVECs)
Shivam Chandel, Rathnakumar Kumaragurubaran, Hemant Giri, and Madhulika Dixit
13.Microvascular Endothelial Glycocalyx Surface Layer Visualization and Quantification
Natascha G. Alves and Jerome W. Breslin
14.Measurement of Blood-Brain Barrier Hyperpermeability Using Evans Blue Extravasation Assay
O'lisa Yaa Waithe, Xu Peng, Ed W. Childs, and Binu Tharakan
15.Assessment of Endothelial Barrier Functions in Extra Embryonic Vasculature of Chick Embryo as an Alternative Model
Jamila Siamwala, Akila Swaminathan, and Suvro Chatterjee
16.Measurement of Transendothelial Electrical Resistance in Blood-Brain Barrier Endothelial Cells
O'lisa Yaa Waithe, Xu Peng, Ed W. Childs, and Binu Tharakan
17.An In Vitro Bilayer Model of Human Primary Retinal Pigment Epithelial and Choroid Endothelial Cells for Permeability Studies
Karthikka Palanisamy and Subbulakshmi Chidambaram
18.Quantifying Adhesion of Inflammatory Cells to the Endothelium In Vitro
Saravanakumar Muthusamy
19.Determination of Tight Junction Integrity in Brain Endothelial Cells Based on Tight Junction Protein Expression
Himakarnika Alluri, Chander Sekhar Peddaboina, and Binu Tharakan
20.Evaluation of Glycolysis and Mitochondrial Function in Endothelial Cells Using the Seahorse Analyzer
Zeinab Y. Motawe, Salma S. Abdelmaboud, and Jerome W. Breslin
21.Evaluation of Tight Junction Integrity in Brain Endothelial Cells using Confocal Microscopy
Himakarnika Alluri, Chander Sekhar Peddaboina, and Binu Tharakan
Subject Index List...
Klappentext
This volume focuses on the latest research methods and techniques used by researchers to study vascular permeability/hyperpermeability in a science laboratory setting. The chapters in this book cover topics such as determination of solute permeability of microvascular endothelial cell monolayers in vitro; evaluation of barrier integrity used in a two-layered microfluidic device mimicking the blood-brain barrier; isolation and culture of human umbilical vein endothelial cells; measurement of blood-brain barrier hyperpermeability using Evans Blue extravasation assay; lymphatic vascular permeability determined from direct measurements of solute flux; intravital imaging of leukocyte-endothelial interaction by intravital multiphoton microscopy; and evaluation of tight junction integrity in brain endothelial cells using confocal microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Vascular Hyperpermeability: Methods and Protocols is a valuable resource that will aid novice researchers with creating new and affordable research projects, and for expert researchers to initiate new strategies and collaborations in their ongoing programs.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
