Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, tranillegalscription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge,
Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms
1. RNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms
Benoit St-Pierre, Samira Mahroug, Gregory Guirimand, Vincent Courdavault and Vincent Burlat
2. Imaging MS analysis in Catharanthus roseus
Kotaro Yamamoto, Katsutoshi Takahashi, Sarah E. O´Connor and Tetsuro Mimura
3. Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle
Chun-Han Su, Bo-Wei Wang, Ewelina P. Dutkiewicz, Cheng-Chih Hsu and Yu-Liang Yang
4. Sample preparation, data acquisition, and data analysis for 15N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus
Ryo Nakabayashi
5. Studying iridoid transport in Catharanthus roseus by grafting
Maisha Farzana, Mohammadamin Shahsavarani, Vincenzo De Luca and Yang Qu
6. Pictet-Spengler reaction for the chemical synthesis of strictosidine
Yingchao Dou, Laurent Evanno, Erwan Poupon and Guillaume Vincent
7. Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids
Pierre Le Pogam, Erwan Poupon, Pierre Champy and Mehdi A. Beniddir
8. Semi-Synthesis of Bis-Indole Alkaloid (–)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis
Alex Gardner and Rodrigo B. Andrade
9. RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus
Emily Amor Stander, Thomas Dugé de Bernonville, Vincent Courdavault
10. Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks
Thomas Dugé de Bernonville, Emily Amor Stander, Géraud Dugé de Bernonville, Sébastien Besseau and Vincent Courdavault
11. Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis
Tuan-Anh Minh Nguyen, Matthew McConnachie, Trinh-Don Nguyen and Thu-Thuy T. Dang
12. Ancestral sequence reconstruction for exploring alkaloid evolution
Benjamin R. Lichman
13. Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus
Mohammadamin Shahsavarani, Maisha Farzana, Vincenzo De Luca and Yang Qu
14. TARGETing tranillegalscriptional regulation in the medicinal plant Catharanthus roseus
Joana G. Guedes, Catarina Leitão, Catarina Meireles, Patrícia Duarte and Mariana Sottomayor
15. Identification and Characterization of Tranillegalscription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus
Sanjay K. Singh, Barunava Patra, Joshua J. Singleton, Yongliang Liu, Priyanka Paul, Xueyi Sui, Nitima Suttipanta, Sitakanta Pattanaik and Ling Yuan
16. From Methylome to Integrative Analysis of Tissue-Specificity
Thomas Dugé de Bernonville, Christian Daviaud, Cristian Chaparro, Jörg Tost and Stéphane Maury
17. Tagging and capture of prenylated CaaX-proteins from plant cell cultures
Iliana Ribeiro, Eric Ducos, Nathalie Giglioli-Guivarc´h and Christelle Dutilleul
18. EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings
Samuel Mortensen, Lauren F. Cole, Diana Bernal-Franco, Suphinya Sathitloetsakun, Erin J. Cram and Carolyn W.T. Lee-Parsons
19. A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus
Konstantinos Koudounas, Ines Carqueijeiro, Pamela Lemos Cruz, Jennifer Perrin, Arnaud Lanoue, Audrey Oudin, Sébastien Besseau and Vincent Courdavault
20. Transient gene expression in Catharanthus roseus flower petals using agroinfiltration
Maite Colinas and Alain Goossens
21. De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants
Priyanka Verma, Shamshad Ahmad Khan and Ajay Kumar Mathur
22. Agrobacterium-mediated in planta transformation in Periwinkle
Dikki Pedenla Bomzan, Shilpashree H.B. and Dinesh A. Nagegowda
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in
C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, tranillegalscription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful
Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, tranillegalscription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge,
Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms
RNA
in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms.- Imaging MS analysis in
Catharanthus roseus.- Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle.- Sample preparation, data acquisition, and data analysis for
15N-labeled and non-labeled monoterpene indole alkaloids in
Catharanthus roseus.- Studying iridoid transport in
Catharanthus roseus by grafting.- Pictet-Spengler reaction for the chemical synthesis of strictosidine.- Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids.- Semi-Synthesis of Bis-Indole Alkaloid (-)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis.- RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus.- Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks.- Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis.- Ancestral sequence reconstruction for exploring alkaloid evolution.- Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in
Catharanthus roseus.- TARGETing tranillegalscriptional regulation in the medicinal plant
Catharanthus roseus.- Identification and Characterization of Tranillegalscription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in
Catharanthus roseus.- From Methylome to Integrative Analysis of Tissue-Specificity.- Tagging and capture of prenylated CaaX-proteins from plant cell cultures.- EASI transformation protocol: An
Agrobacterium-mediated transient transformation protocol for
Catharanthus roseus seedlings.- A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of
Catharanthus roseus.- Transient gene expression in
Catharanthus roseus flower petals using agroinfiltration.- De novo shoot bud induction from the
Catharanthus roseus leaf explants and
Agrobacterium tumefaciens mediated technique to raise transgenic plants.-
Agrobacterium-mediated
in planta transformation in Periwinkle.
Inhaltsverzeichnis
RNA in situ hybridization on paraffin-sections to characterize the multicellular compartmentation of plant secondary metabolisms.- Imaging MS analysis in Catharanthus roseus.- Surface-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry (SALDI-IMS)-Based Detection of Vinca Alkaloids Distribution in the Petal of Madagascar Periwinkle.- Sample preparation, data acquisition, and data analysis for 15N-labeled and non-labeled monoterpene indole alkaloids in Catharanthus roseus.- Studying iridoid transport in Catharanthus roseus by grafting.- Pictet-Spengler reaction for the chemical synthesis of strictosidine.- Implementation of a MS/MS spectral library for Monoterpene Indole Alkaloids.- Semi-Synthesis of Bis-Indole Alkaloid (-)-Melodinine K Enabled by a Combination of Biotransformation and Chemical Synthesis.- RNA-seq analysis towards monoterpene indole alkaloid biosynthetic pathway elucidation in Catharanthus roseus.- Predicting Monoterpene Indole Alkaloid related genes from expression data with artificial neural networks.- Discovery and characterization of oxidative enzymes in monoterpenoid indole alkaloid biosynthesis.- Ancestral sequence reconstruction for exploring alkaloid evolution.- Generating an EMS mutant population and rapid mutant screening by thin layer chromatography enables the studies of monoterpenoid indole alkaloids biosynthesis in Catharanthus roseus.- TARGETing tranillegalscriptional regulation in the medicinal plant Catharanthus roseus.- Identification and Characterization of Tranillegalscription Factors Regulating Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus.- From Methylome to Integrative Analysis of Tissue-Specificity.- Tagging and capture of prenylated CaaX-proteins from plant cell cultures.- EASI transformation protocol: An Agrobacterium-mediated transient transformation protocol for Catharanthus roseus seedlings.- A rapid and efficient vacuum-based Agroinfiltration protocol for transient gene overexpression in leaves of Catharanthus roseus.- Transient gene expression in Catharanthus roseus flower petals using agroinfiltration.- De novo shoot bud induction from the Catharanthus roseus leaf explants and Agrobacterium tumefaciens mediated technique to raise transgenic plants.- Agrobacterium-mediated in planta transformation in Periwinkle.
Klappentext
This volume provides updated technical approaches that have been developed to characterize monoterpene indole alkaloid metabolism in C. roseus from metabolite/gene product localization, alkaloid chemical synthesis, candidate gene prediction, tranillegalscription factor characterization up to functional genomic tools based on gene overexpression. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Catharanthus roseus: Methods and Protocols aims to be a guidebook to all researchers working at characterizing alkaloid biosynthesis and more broadly specialized metabolisms
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
