Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume provides researchers with up-to-date protocols and takes aclose look at current research and promising applications. Optogenetics:Methods and Protocols contains a collection of recently developed technicalprotocols on optogenetic applications in neuroscience, brain mapping, treatmentof neurological disorders, and restoration of visual function. Severalintroductory and discussion chapters offer a wide overview about sources anddiversity of optogenetic tools, design strategies, and potential applicationsin other fields like plant research. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Protocols, deliversan interdisciplinary view of optogenetic applications and its potential todevelop as a valuable tool for basic research, as well as biomedical andbiotechnological applications.
1. Optogenetics:Basic Concepts and Their Development
Yong Ku Cho and Dan Li
2. NaturalResources for Optogenetic Tools
Tilo Mathes
3. Algal Photobiology: A Rich Source ofUnusual Light Sensitive Proteins for Synthetic Biology and Optogenetics
Arash Kianianmomeni and ArminHallmann
4. Reversible Photoregulation of geneExpression and Translation
Shinzi Ogasawara
5. Controlling Protein Activity andDegradation Using Blue-Light
Anne P. Lutz, Christian Renicke, andChristof Taxis
6. Photo-Control of Protein Function UsingPhotoactive Yellow Protein
Jakeb M. Reis and G. Andrews Woolley
7. A Fluorometric Activity Assay forLight-Regulated Cyclic-Nucleotide-Monophosphate Actuators
Charlotte Helene Chumacher, Heinz G.Körschen, Christopher Nicol, Carlos Gasser, ReinhardSeifert, Martin Schwärzel, and Andreas Möglich
8. Optogenetic Control of Pancreatic Islets
Thomas M. Reinbothe and Inês G.Mollet
9. Optogenetics in Plants: Red/Far-Red LightControl of Gene Expression
Rocio Ochoa-Fernandez, Sophia L.Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Müller, Wilfried Weber, andMatias D. Zurbriggen
10. Perfect Channelrhodopsins: A User Guide
Jonas Wietek and Matthias Prigge
11. Optogenetics in Drosophila Neuroscience
Thomas Riemensperger, Robert J.Kittel, and André Fiala
12. Optogenetic Control of Mammalian IonChannels with Chemical Photoswitches
Damien Lemoine, Romain Durand-deCuttoli, and Alexandre Mourot
13. Optogenetic Modulation of LocomotorActivity on Free Behaving Rats
Kedi Xu, Jiacheng Zhang, SongchaoGuo, and Xiaoxiang Zheng
14. Combines Optogenetic and ChemogeneticControl of Neurons
Ken Berglund, Jack K. Tung, BryanHigashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender
15. Intracranial Injection of an OptogeneticsViral Vector Followed by Optical Cannula Implantation for Neural Stimulation inRat Brain Cortex
Christopher Pawela, Edgar DeYoe, andRamin Pashaie
16. An Optomized Calcium-Phosphate TransfectionMethod for Characterizing Genetically Encoded Tools in Primary Neurons
Shiyao Wang and Yong Ku Cho
17. Optogenetic Approaches for Mesoscopic BrainMapping
Michael Kyweriga and Majid H.Mohajerani
18. Optogenetic Tools for Confined Stimulationin Deep Brain Structures
Alexandre Castonguay, SébastienThomas, Frédéric Lesage, and Christian Casanova
19. Remote Patterning of Transgene ExpressionUsing Near Infrared-Responsive Plasmonic Hydrogels
Francisco Martín-Saavedra and NuriaVilaboa
20. Optogenetic Light Crafting Tools for theControl of Cardiac Arrhythmias
Claudia Richter, Jan Christoph,Stephan E. Lehnart, and Stefan Luther
21. Inscribing Optocal Excitability toNon-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in PrimaryCardiac Fibroblasts
Jinzhu Yu and Emilia Entcheva
22. Optogenetic Engineering of AtrialCardiomyocytes
Iolanda Feola, Alexander Teplenin,Antoine A.F. de Vries, and Daniël A. Pijnappels
23. A Multi-Channel Recording System withOptical Stimulation for Closed-Loop Optogenetic Experiments
Carmen Bartic, Francesco P.Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova
24. Optogenetic Control of Fibroblast GrowthFactor Receptor Signaling
Nury Kim, Jin Man Kim, and Won Do Heo
25. Protein Inactivation by OptogeneticTrapping in Living Cells
Hyerim Park, Sangkyu Lee, and Won DoHeo
26. Optogenetic Manipulation of SelectiveNeural Activity in Free-Moving DrosophilaAdults
Po-Yen Hsiao, Ming-Chin Wu, Yen-YinLin, Chein-Chung Fu, and Ann-Shyn Chiang
27. Guidelines for Photoreceptor Engineering
Thea Ziegler, Charlotte HeleneSchumacher, and Andreas Möglich
This volume provides researchers with up-to-date protocols and takes aclose look at current research and promising applications. Optogenetics:Methods and Protocols contains a collection of recently developed technicalprotocols on optogenetic applications in neuroscience, brain mapping, treatmentof neurological disorders, and restoration of visual function. Severalintroductory and discussion chapters offer a wide overview about sources anddiversity of optogenetic tools, design strategies, and potential applicationsin other fields like plant research. Written in the highly successful Methodsin Molecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.
Cutting edge and thorough, Optogenetic: Methods and Protocols, deliversan interdisciplinary view of optogenetic applications and its potential todevelop as a valuable tool for basic research, as well as biomedical andbiotechnological applications.
Optogenetics:Basic Concepts and Their Development.- NaturalResources for Optogenetic Tools.- Algal Photobiology: A Rich Source ofUnusual Light Sensitive Proteins for Synthetic Biology and Optogenetics.- Reversible Photoregulation of geneExpression and Translation.- Controlling Protein Activity andDegradation Using Blue-Light.- Photo-Control of Protein Function UsingPhotoactive Yellow Protein.- A Fluorometric Activity Assay forLight-Regulated Cyclic-Nucleotide-Monophosphate Actuators.- Optogenetic Control of Pancreatic Islets.- Optogenetics in Plants: Red/Far-Red LightControl of Gene Expression.- Perfect Channelrhodopsins: A User Guide.- Optogenetics in Drosophila Neuroscience.- Optogenetic Control of Mammalian IonChannels with Chemical Photoswitches.- Optogenetic Modulation of LocomotorActivity on Free Behaving Rats.- Combines Optogenetic and ChemogeneticControl of Neurons.- Intracranial Injection ofan OptogeneticsViral Vector Followed by Optical Cannula Implantation for Neural Stimulation inRat Brain Cortex.- An Optomized Calcium-Phosphate TransfectionMethod for Characterizing Genetically Encoded Tools in Primary Neurons.- Optogenetic Approaches for Mesoscopic BrainMapping.- Optogenetic Tools for Confined Stimulationin Deep Brain Structures.- Remote Patterning of Transgene ExpressionUsing Near Infrared-Responsive Plasmonic Hydrogels.- Optogenetic Light Crafting Tools for theControl of Cardiac Arrhythmias.- Inscribing Optocal Excitability toNon-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in PrimaryCardiac Fibroblasts.- Optogenetic Engineering of AtrialCardiomyocytes.- A Multi-Channel Recording System withOptical Stimulation for Closed-Loop Optogenetic Experiments.- Optogenetic Control of Fibroblast GrowthFactor Receptor Signaling.- Protein Inactivation by OptogeneticTrapping in Living Cells.- Optogenetic Manipulation of SelectiveNeural Activity in Free-Moving DrosophilaAdults.- Guidelines for Photoreceptor Engineering.
Inhaltsverzeichnis
1.
Optogenetics:
Basic Concepts and Their Development
Yong Ku Cho and Dan Li
2.
Natural
Resources for Optogenetic Tools
Tilo Mathes
3. Algal Photobiology: A Rich Source of
Unusual Light Sensitive Proteins for Synthetic Biology and Optogenetics
Arash Kianianmomeni and Armin
Hallmann
4. Reversible Photoregulation of gene
Expression and Translation
Shinzi Ogasawara
5. Controlling Protein Activity and
Degradation Using Blue-Light
Anne P. Lutz, Christian Renicke, and
Christof Taxis
6. Photo-Control of Protein Function Using
Photoactive Yellow Protein
Jakeb M. Reis and G. Andrews Woolley
7. A Fluorometric Activity Assay for
Light-Regulated Cyclic-Nucleotide-Monophosphate Actuators
Charlotte Helene Chumacher, Heinz G.
Körschen, Christopher Nicol, Carlos Gasser, Reinhard
Seifert, Martin Schwärzel, and Andreas Möglich
8. Optogenetic Control of Pancreatic Islets
Thomas M. Reinbothe and Inês G.
Mollet
9. Optogenetics in Plants: Red/Far-Red Light
Control of Gene Expression
Rocio Ochoa-Fernandez, Sophia L.
Samodelov, Simon M. Brandl, Elke Wehinger, Konrad Müller, Wilfried Weber, and
Matias D. Zurbriggen
10. Perfect Channelrhodopsins: A User Guide
Jonas Wietek and Matthias Prigge
11. Optogenetics in Drosophila Neuroscience
Thomas Riemensperger, Robert J.
Kittel, and André Fiala
12. Optogenetic Control of Mammalian Ion
Channels with Chemical Photoswitches
Damien Lemoine, Romain Durand-de
Cuttoli, and Alexandre Mourot
13. Optogenetic Modulation of Locomotor
Activity on Free Behaving Rats
Kedi Xu, Jiacheng Zhang, Songchao
Guo, and Xiaoxiang Zheng
14. Combines Optogenetic and Chemogenetic
Control of Neurons
Ken Berglund, Jack K. Tung, Bryan
Higashikubo, Robert E. Gross, Christopher I. Moore, and Ute Hochgeschwender
15. Intracranial Injection of an Optogenetics
Viral Vector Followed by Optical Cannula Implantation for Neural Stimulation in
Rat Brain Cortex
Christopher Pawela, Edgar DeYoe, and
Ramin Pashaie
16. An Optomized Calcium-Phosphate Transfection
Method for Characterizing Genetically Encoded Tools in Primary Neurons
Shiyao Wang and Yong Ku Cho
17. Optogenetic Approaches for Mesoscopic Brain
Mapping
Michael Kyweriga and Majid H.
Mohajerani
18. Optogenetic Tools for Confined Stimulation
in Deep Brain Structures
Alexandre Castonguay, Sébastien
Thomas, Frédéric Lesage, and Christian Casanova
19. Remote Patterning of Transgene Expression
Using Near Infrared-Responsive Plasmonic Hydrogels
Francisco Martín-Saavedra and Nuria
Vilaboa
20. Optogenetic Light Crafting Tools for the
Control of Cardiac Arrhythmias
Claudia Richter, Jan Christoph,
Stephan E. Lehnart, and Stefan Luther
21. Inscribing Optocal Excitability to
Non-Excitable Cardiac Cells: Viral Delivery of Optogenetic Tools in Primary
Cardiac Fibroblasts
Jinzhu Yu and Emilia Entcheva
22. Optogenetic Engineering of Atrial
Cardiomyocytes
Iolanda Feola, Alexander Teplenin,
Antoine A.F. de Vries, and Daniël A. Pijnappels
23. A Multi-Channel Recording System with
Optical Stimulation for Closed-Loop Optogenetic Experiments
Carmen Bartic, Francesco P.
Battaglia, Ling Wang, Thoa T. Nguyen, Henrique Cabral, and Zaneta Navratilova
24. Optogenetic Control of Fibroblast Growth
Factor Receptor Signaling
Nury Kim, Jin Man Kim, and Won Do Heo
25. Protein Inactivation by Optogenetic
Trapping in Living Cells
Hyerim Park, Sangkyu Lee, and Won Do
Heo
26. Optogenetic Manipulation of Selective
Neural Activity in Free-Moving Drosophila
Adults
Po-Yen Hsiao, Ming-Chin Wu, Yen-Yin
Lin, Chein-Chung Fu, and Ann-Shyn Chiang
27. Guidelines for Photoreceptor Engineering
Thea Ziegler, Charlotte Helene
Schumacher, and Andreas Möglich
Klappentext
This volume provides researchers with up-to-date protocols and takes anclose look at current research and promising applications. Optogenetics:nMethods and Protocols contains a collection of recently developed technicalnprotocols on optogenetic applications in neuroscience, brain mapping, treatmentnof neurological disorders, and restoration of visual function. Severalnintroductory and discussion chapters offer a wide overview about sources andndiversity of optogenetic tools, design strategies, and potential applicationsnin other fields like plant research. Written in the highly successful Methodsnin Molecular Biology series format, chapters include introductions to theirnrespective topics, lists of the necessary materials and reagents, step-by-step,nreadily reproducible laboratory protocols, and tips on troubleshooting andnavoiding known pitfalls.nnCutting edge and thorough, Optogenetic: Methods and Protocols, deliversnan interdisciplinary view of optogenetic applications and its potential tondevelop as a valuable tool for basic research, as well as biomedical andnbiotechnological applications.nnnn
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
