Describes the instruments, samples, protocols, and analyses that belong to cryo-EM. This title emphasizes the relatedness of the ideas, instrumentation, and methods underlying cryo-EM approaches which allow practitioners to easily move between them.
Cryo-EM Part A: Sample Preparation and Data Collection is dedicated to a deillegalscription of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
Historical perspective
3D reconstruction from electron micrographs: a personal account of its development
David DeRosier
- Preparation of 2-D crystals
Henning Stahlberg
- Helical Crystallization of Soluble and Membrane Binding Proteins
Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie
- Plunge Freezing for Electron Cryomicroscopy
Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen
- A Practical Guide to the Use of Monolayer Purification and Affinity Grids
Thomas Walz, Deborah F. Kelly, Danijela Dukovski
- GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Holger Stark
- Cryo-negative staining of macromolecular assemblies
Sacha De Carlo, Holger Stark
- Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
Fred J. Sigworth, Liguo Wang
- Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
Mark S. Ladinsky
- Site-Specific Biomolecule Labeling with Gold Clusters
Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld
- How to Operate a Cryo-Electron Microscope
Huilin Li, Jingchuan Sun
- Collecting electron crystallographic data of two-dimensional protein crystals
Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng
- Automated Data Collection for Electron Microscopic Tomography
David Agard, Shawn Q. Zheng, J. W. Sedat
- Correlated Light and Electron Cryomicroscopy
Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz
- Phase Plates for Transmission Electron Microscopy
Radostin Danev, Kuniaki Nagayama
- Radiation damage in electron cryomicroscopy
John L. Rubinstein, Lindsay A. Baker
Inhaltsverzeichnis
Historical perspective
3D reconstruction from electron micrographs: a personal account of its development
David DeRosier
- Preparation of 2-D crystals
Henning Stahlberg
- Helical Crystallization of Soluble and Membrane Binding Proteins
Elizabeth M. Wilson-Kubalek, Christopher Arthur, Joshua S. Chappie
- Plunge Freezing for Electron Cryomicroscopy
Alasdair W. McDowall, Megan J. Dobro, Linda A. Melanson, Grant J. Jensen
- A Practical Guide to the Use of Monolayer Purification and Affinity Grids
Thomas Walz, Deborah F. Kelly, Danijela Dukovski
- GraFix: Stabilization of fragile macromolecular complexes for single particle cryo-EM
Holger Stark
- Cryo-negative staining of macromolecular assemblies
Sacha De Carlo, Holger Stark
- Liposomes on a streptavidin crystal: a system to study membrane proteins by cryo-EM
Fred J. Sigworth, Liguo Wang
- Micromanipulator-Assisted Vitreous Cryosectioning and Sample Preparation by High-Pressure Freezing
Mark S. Ladinsky
- Site-Specific Biomolecule Labeling with Gold Clusters
Christopher J. Ackerson, Richard D. Powell, James F. Hainfeld
- How to Operate a Cryo-Electron Microscope
Huilin Li, Jingchuan Sun
- Collecting electron crystallographic data of two-dimensional protein crystals
Thomas Walz, Richard K. Hite, Andreas D. Schenk, Zongli Li, Yifan Cheng
- Automated Data Collection for Electron Microscopic Tomography
David Agard, Shawn Q. Zheng, J. W. Sedat
- Correlated Light and Electron Cryomicroscopy
Grant J. Jensen, Ariane Briegel, Songye Chen, Abraham J. Kosterc, Jürgen M. Plitzkod, Cindi L. Schwartz
- Phase Plates for Transmission Electron Microscopy
Radostin Danev, Kuniaki Nagayama
- Radiation damage in electron cryomicroscopy
John L. Rubinstein, Lindsay A. Baker
Klappentext
This volume is dedicated to a deillegalscription of the instruments, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the ideas, instrumentation, and methods underlying all cryo-EM approaches, which allow practitioners to easily move between them. Within each section, the articles are ordered according to the most common symmetry of the sample to which their methods are applied.
