Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as
in vivo therapeutic applications. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.
Part I: Understanding Signaling Events and Patterns
1. TASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy
Nicholas Walczak, Jacob Beal, Jesse Tordoff, and Ron Weiss
2. Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities
Shay Carter, Joshua Hislop, Joshua Hsu, Jeremy J. Velazquez, and Mo R. Ebrahimkhani
3. A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut
Svetlana Ulyanchenko and Jordi Guiu
Part II: Programming Signaling Events and Patterns
4. Reconstitution of Morphogen Signaling Gradients in Cultured Cells
Julia S. Kim, Michael Pineda, and Pulin Li
5. Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands
Megan L. Rexius-Hall, Nethika R. Ariyasinghe, and Megan L. McCain
6. Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives
Thomas Richardson, Shibin Mathew, Connor Wiegand, Kevin Pietz, Joseph Candiello, K Ravikumar, and Ipsita Banerjee
7. Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model
Vasilios Nanos and Michael Levin
8. Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells
Ashley R.G. Libby, David A. Joy, and Todd C. McDevitt
Part III: Early Developmental Engineering
9. Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells
George Britton, Sapna Chhabra, Joseph Massey, and Aryeh Warmflash
10. Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos
Kerim Anlas, Peter Baillie-Johnson, Krisztina Arató, David A. Turner, and Vikas Trivedi
Part IV: Organoids, Tissue Barriers, and Disease Models
11. A Synergistic Engineering Approach to Build Human Brain Spheroids
Djuna von Maydell and Mehdi Jorfi
12. Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids
Idoia Lucía Selfa, Maria Gallo, Núria Montserrat, and Elena Garreta
13. Methods for Controlled Induction of Singular Rosette Cytoarchitecture within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies
Alireza Aghayee and Randolph Ashton
14. 3D Self-Organized Human Blood-Brain Barrier in a Microfluidic Chip
Marco Campisi, Sei Hien Lim, Valeria Chiono, and Roger Kamm
15. Modeling the Complexity of the Metastatic Niche Ex Vivo
Amanda M. Clark
16. Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression
Harpinder Saini and Mehdi Nikkhah
Part V: In Vivo Therapeutic Applications
17. A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization
Amy E. Emerson, Emily M. Slaby, and Jessica D. Weaver
18. High Throughput Production of Platelet-Like Particles
Kylie M. Persson, Pauline V. Kneller, Mark W. Livingston, Lucas M. Bush, and Tara L. Deans
This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as
in vivo therapeutic applications. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.
This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as
in vivo therapeutic applications. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.
TASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy.- Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities.- A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut.- Reconstitution of Morphogen Signaling Gradients in Cultured Cells.- Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands.- Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives.- Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model.- Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells.- Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells.- Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos.- A Synergistic Engineering Approach toBuild Human Brain Spheroids.- Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids.- Methods for Controlled Induction of Singular Rosette Cytoarchitecture within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies.- 3D Self-Organized Human Blood-Brain Barrier in a Microfluidic Chip.- Modeling the Complexity of the Metastatic Niche Ex Vivo.- Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression.- A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization.- High Throughput Production of Platelet-Like Particles.
Inhaltsverzeichnis
Part I: Understanding Signaling Events and Patterns
1. TASBE Image Analytics: A Processing Pipeline for Quantifying Cell Organization from Fluorescent Microscopy
Nicholas Walczak, Jacob Beal, Jesse Tordoff, and Ron Weiss
2. Neighborhood Impact Factor to Study Cell-Fate Decision-Making in Cellular Communities
Shay Carter, Joshua Hislop, Joshua Hsu, Jeremy J. Velazquez, and Mo R. Ebrahimkhani
3. A Quantitative Lineage-Tracing Approach to Understand Morphogenesis in Gut
Svetlana Ulyanchenko and Jordi Guiu
Part II: Programming Signaling Events and Patterns
4. Reconstitution of Morphogen Signaling Gradients in Cultured Cells
Julia S. Kim, Michael Pineda, and Pulin Li
5. Engineering Shape-Controlled Microtissues on Compliant Hydrogels with Tunable Rigidity and Extracellular Matrix Ligands
Megan L. Rexius-Hall, Nethika R. Ariyasinghe, and Megan L. McCain
6. Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives
Thomas Richardson, Shibin Mathew, Connor Wiegand, Kevin Pietz, Joseph Candiello, K Ravikumar, and Ipsita Banerjee
7. Rewiring Endogenous Bioelectric Circuits in the Xenopus laevis Embryo Model
Vasilios Nanos and Michael Levin
8. Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells
Ashley R.G. Libby, David A. Joy, and Todd C. McDevitt
Part III: Early Developmental Engineering
9. Fate-Patterning of 2D Gastruloids and Ectodermal Colonies Using Micropatterned Human Pluripotent Stem Cells
George Britton, Sapna Chhabra, Joseph Massey, and Aryeh Warmflash
10. Gastruloids: Embryonic Organoids from Mouse Embryonic Stem Cells to Study Patterning and Development in Early Mammalian Embryos
Kerim Anlas, Peter Baillie-Johnson, Krisztina Arató, David A. Turner, and Vikas Trivedi
Part IV: Organoids, Tissue Barriers, and Disease Models
11. A Synergistic Engineering Approach to Build Human Brain Spheroids
Djuna von Maydell and Mehdi Jorfi
12. Directed Differentiation of Human Pluripotent Stem Cells for the Generation of High-Order Kidney Organoids
Idoia Lucía Selfa, Maria Gallo, Núria Montserrat, and Elena Garreta
13. Methods for Controlled Induction of Singular Rosette Cytoarchitecture within Human Pluripotent Stem Cell-Derived Neural Multicellular Assemblies
Alireza Aghayee and Randolph Ashton
14. 3D Self-Organized Human Blood-Brain Barrier in a Microfluidic Chip
Marco Campisi, Sei Hien Lim, Valeria Chiono, and Roger Kamm
15. Modeling the Complexity of the Metastatic Niche Ex Vivo
Amanda M. Clark
16. Fabrication Method of a High-Density Co-Culture Tumor-Stroma Platform to Study Cancer Progression
Harpinder Saini and Mehdi Nikkhah
Part V: In Vivo Therapeutic Applications
17. A Method for Organoid Transplantation and Whole-Mount Visualization of Post-Engraftment Vascularization
Amy E. Emerson, Emily M. Slaby, and Jessica D. Weaver
18. High Throughput Production of Platelet-Like Particles
Kylie M. Persson, Pauline V. Kneller, Mark W. Livingston, Lucas M. Bush, and Tara L. Deans
Klappentext
This detailed book explores techniques for understanding and engineering programs that naturally control and drive formation of tissues and organs in order to open powerful opportunities to produce physiologically relevant tissues of interest, generate models to study human disease, and set the path for the manufacturing of advanced tissue and organs. Beginning with chapters to help understand signaling events and patterns in morphogenetic systems, the volume continues by covering programming signaling events and patterns to drive morphogenesis, techniques for engineering organoids, tissue barriers, and disease models, as well as in vivo therapeutic applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
n
Authoritative and cutting-edge, Programmed Morphogenesis: Methods and Protocols aims not only to communicate knowledge but also to inspire approaches to new challenges and to empower readers with the capability to approach those challenges.
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
