This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy.
This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an "integrative taxonomy" framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date,
Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.
Plant Taxonomy: A Historical Perspective, Current Challenges, and Perspectives.- Guidelines for the Choice of Sequences for Molecular Plant Taxonomy.- Isolation and Purification of DNA from Complicated Biological Samples.- Herbarium Specimens: A Treasure for DNA Extraction, an Update.- Sequencing of Complete Chloroplast Genomes.- Utility of the Mitochondrial Genome in Plant Taxonomic Studies.- Nuclear Ribosomal RNA Genes: ITS Region.- Plant DNA Barcoding Principles and Limits: A Case Study in the Genus Vanilla.- High-Throughput Genotyping Technologies in Plant Taxonomy.- Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny.- Development of Microsatellite Markers Using Next-Generation Sequencing.- Amplified Fragment Length Polymorphism: Applications and Recent Developments.- Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques.- Inter Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers.- Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity.- Introduction to Population Genomics Methods.- The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants.- Molecular Cytogenetics (FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization.- GISH: Resolving Interspecific and Intergeneric Hybrids.
Inhaltsverzeichnis
1. Plant Taxonomy: A Historical Perspective, Current Challenges, and Perspectives
Germinal Rouhan and Myriam Gaudeul
2. Guidelines for the Choice of Sequences for Molecular Plant Taxonomy
Pascale Besse
3. Isolation and Purification of DNA from Complicated Biological Samples
Ruslan Kalendar, Svetlana Boronnikova, and Mervi Seppänen
4. Herbarium Specimens: A Treasure for DNA Extraction, an Update
Lenka Záveská Drábková
5. Sequencing of Complete Chloroplast Genomes
Berthold Heinze
6. Utility of the Mitochondrial Genome in Plant Taxonomic Studies
Jérôme Duminil and Guillaume Besnard
7. Nuclear Ribosomal RNA Genes: ITS Region
Pascale Besse
8. Plant DNA Barcoding Principles and Limits: A Case Study in the Genus Vanilla
Pascale Besse, Denis Da Silva, and Michel Grisoni
9. High-Throughput Genotyping Technologies in Plant Taxonomy
Monica F. Danilevicz, Cassandria T. Fernandez, Jacob I. Marsh, Philipp E. Bayer, and David Edwards
10. Genotyping-by-Sequencing Technology in Plant Taxonomy and Phylogeny
Félicien Favre, Cyril Jourda, Pascale Besse, and Carine Charron
11. Development of Microsatellite Markers Using Next-Generation Sequencing
Hélène Vignes and Ronan Rivallan
12. Amplified Fragment Length Polymorphism: Applications and Recent Developments
Thotten Elampilay Sheeja, Illathidath Payatatti Vijesh Kumar, Ananduchandra Giridhari, Divakaran Minoo, Muliyar Krishna Rajesh, and Kantipudi Nirmal Babu
13. Randomly Amplified Polymorphic DNA (RAPD) and Derived Techniques
Kantipudi Nirmal Babu, Thotten Elampilay Sheeja, Divakaran Minoo, Muliyar Krishna Rajesh, Kukkamgai Samsudeen, Erinjery Jose Suraby, and Illathidath Payatatti Vijesh Kumar
14. Inter Simple Sequence Repeats (ISSR), Microsatellite-Primed Genomic Profiling Using Universal Primers
Chrissen E.C. Gemmill and Ella R.P. Grierson
15. Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity
Ruslan Kalendar, Alexander Muterko, and Svetlana Boronnikova
16. Introduction to Population Genomics Methods
Thibault Leroy and Quentin Rougemont
17. The Application of Flow Cytometry for Estimating Genome Size, Ploidy Level Endopolyploidy, and Reproductive Modes in Plants
Jaume Pellicer, Robin F. Powell, and Ilia J. Leitch
18. Molecular Cytogenetics (FISH and Fluorochrome Banding): Resolving Species Relationships and Genome Organization
Sonja Siljak-Yakovlev, Fatima Pustahija, Vedrana Vicic Bockor, and Odile Robin
19. GISH: Resolving Interspecific and Intergeneric Hybrids
Nathalie Piperidis
Klappentext
This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an ¿integrative taxonomy¿ framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date,Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
