It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms.
This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.
CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana.- Strand-Specific RNA-Seq Applied to Malaria Samples.- Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning.- Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting.- Quantitative Analysis of Plant miRNA Primary Tranillegalscripts.- A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq.- Analysis of RBP Regulation and Co-Regulation of mRNA 3' UTR Regions in a Luciferase Reporter System.- Extraction of Small RNAs by Titanium Dioxide Nanofibers.- Identification of MicroRNAs and Natural Antisense Tranillegalscript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data.- Purification and Analysis of Chloroplast RNAs in Arabidopsis.- In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes.- Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs.- Isolation of Insect Bacteriocytes as a Platform for Tranillegalscriptomic Analyses.- Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue.- Studying RNA-Protein Interaction Using Riboproteomics.- Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation.
Inhaltsverzeichnis
1. CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in Nicotiana benthamiana
Veerendra Sharma, Wenguang Zheng, Jun Huang, and David E. Cook
2. Strand-Specific RNA-Seq Applied to Malaria Samples
Xueqing Maggie Lu and Karine Le Roch
3. Laser Microdissection of Cells and Isolation of High-Quality RNA after Cryosectioning
Marta Barcala, Carmen Fenoll, and Carolina Escobar
4. Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting
Lena Krüßel, Steffen Ostendorp, Anna Ostendorp, and Julia Kehr
5. Quantitative Analysis of Plant miRNA Primary Tranillegalscripts
Jakub Dolata, Andrzej Zielezinski, Agata Stepien, Katarzyna Kruszka, Dawid Bielewicz, Andrzej Pacak, Artur Jarmolowski, Wojciech Karlowski, and Zofia Szweykowska-Kulinska
6. A Revised Adaptation of the Smart-Seq2 Protocol for Single Nematode RNA-Seq
Dennis Chang, Lorrayne Serra, Dihong Lu, Ali Mortazavi, and Adler Dillman
7. Analysis of RBP Regulation and Co-Regulation of mRNA 3' UTR Regions in a Luciferase Reporter System
Erin L. Sternburg and Fedor V. Karginov
8. Extraction of Small RNAs by Titanium Dioxide Nanofibers
Luis A. Jimenez and Wenwan Zhong
9. Identification of MicroRNAs and Natural Antisense Tranillegalscript-Originated Endogenous siRNAs from Small-RNA Deep Sequencing Data
Weixiong Zhang, Xuefeng Zhou, Xiang Zhou, and Jing Xia
10. Purification and Analysis of Chloroplast RNAs in Arabidopsis
Huan Wang and Hailing Jin
11. In Situ Detection of Mature miRNAs in Plants Using LNA-Modified DNA Probes
Xiaozhen Yao, Hai Huang, and Lin Xu
12. Northern Blotting Technique for Detection and Expression Analysis of mRNAs and Small RNAs
Ankur R. Bhardwaj, Ritu Pandey, Manu Agarwal, and Surekha Katiyar-Agarwal
13. Isolation of Insect Bacteriocytes as a Platform for Tranillegalscriptomic Analyses
Mélanie Ribeiro Lopes, Pierre Simonet, Gabrielle Duport, Karen Gaget, Séverine Balmand, Akiko Sugio, Jean-Christophe Simon, Nicolas Parisot, and Federica Calevro
14. Small RNA Isolation and Library Construction for Expression Profiling of Small RNAs from Neurospora crassa and Fusarium oxysporum and Analysis of Small RNAs in Fusarium oxysporum-Infected Plant Root Tissue
Shou-Qiang Ouyang, Gyungsoon Park, Hui-Min Ji, and Katherine A. Borkovich
15. Studying RNA-Protein Interaction Using Riboproteomics
Sonali Chaturvedi and A.L.N. Rao
16. Small RNA Extraction and Quantification of Isolated Fungal Cells from Plant Tissue by the Sequential Protoplastation
Qiang Cai and Hailing Jin
Klappentext
This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
n
Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
