Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume provides a practical guide providing step-by-step protocol to explore vaccines for farm and companion animals, as well as for fish and insects. Divided into three volumes, Volume 2: Vaccines for Veterinary Diseases guides readers through veterinary vaccines, vaccines for poultry, vaccines for farm animals, and vaccines for veterinary parasites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and practical,
Vaccine Design: Methods and Protocols, Second Edition, Volume 2: Vaccines for Veterinary Diseases aims to be a useful practical guide to researchers to help further their study in this field.
PART I VETERINARY VACCINES: INTRODUCTION
1. Challenges in Veterinary Vaccine Development
Sunil Thomas, Ann Abraham, Alina Rodríguez-Mallon, Sasimanas Unajak, and John P. Bannantine
PART II TRENDS IN VETERINARY VACCINES
2. Mammalian Cell Culture as a Platform for Veterinary Vaccines
Thailín Lao González, Ileanet Ávalos Olivera, and Alina Rodríguez-Mallon
3. Avian Paramyxoviruses as Vectors for Vaccine Development
Shin-Hee Kim, Edris Shirvani, and Siba Samal
PART III VACCINES FOR POULTRY
4. Reverse Genetics and its Usage in the Development of Vaccine against Poultry Diseases
Barnali Nath, Sudhir Morla, and Sachin Kumar
PART IV VACCINES FOR FARM ANIMALS
5. Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies Paratuberculosis Vaccines
John P. Bannantine, Tuhina Gupta, Denise K. Zinniel, Ahmed Hikal, Frederick D. Quinn, and Raul G. Barletta
6. Recombinant Escherichia coli Cell lysates as a low-cost alternative for vaccines against veterinary clostridial diseases
Clóvis Moreira Jr, Gustavo Marçal Schmidt Garcia Moreira, Rafael Amaral Donassolo, Marcos Roberto Alves Ferreira, Mariliana Luiza Ferreira Alves, Rafael Rodrigues Rodrigues, and Fabricio Rochedo Conceição
7. Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli
Mariliana Luiza Ferreira Alves, Gustavo Marçal Schmidt Garcia Moreira, Marcos Roberto Alves Ferreira, Rafael Amaral Donassolo1, Clóvis Moreira Jr, Rafael Rodrigues Rodrigues, and Fabricio Rochedo Conceição
PART V VACCINES FOR VETERINARY PARASITES
8. Macrophage Stimulation as a Useful Approach for Immunoscreening of Potential Vaccine Candidates against Toxoplasma gondii and Neospora caninum Infections
Ragab M. Fereig and Yoshifumi Nishikawa
PART VI VACCINES FOR FISH
9. Fundamentals of Fish Vaccination
Megha Kadam Bedekar and Sajal Kole
10. Teleost Fish as an Experimental Model for Vaccine Development
Marco Antonio de Andrade Belo and Ives Charlie-Silva
11. Development of Nano-conjugated DNA Vaccine against Edwardsiellosis Disease in Fish
Megha Kadam Bedekar and Sajal Kole
12. Development and Evaluation of DNA Vaccine against Salmonid Alphavirus
Chia-Jung Chang
13. Novel Vaccine Development for Fish Culture Based on the Multiepitope Concept
Sasimanas Unajak, Ansaya Pumchan, Sittiruk Roytrakul, Orathai Sawatdichaikul,
and Nontawith Areechon
14. Testing Novel Inactivation Methods and Adjuvants for Vaccines against Streptococcus Agalactiae in Nile Tilapia Oreochromis niloticus
Fernando Carlos Ramos-Espinoza, Victor Alexander Cueva-Quiroz, Jefferson Yunis-Aguinaga, Norquis Caled Alvarez-Rubio, Nicoli Paganoti de Mello, and Julieta Rodini Engrácia de Moraes
PART VII VACCINES AGAINST TICKS
15. Anti-tick Vaccines: Current Advances and Future Prospects
Dennis Muhanguzi, Christian Ndekezi, Joseph Nkamwesiga, Shewit Kalayou, Sylvester Ochwo, Moses Vuyani, and Magambo Phillip Kimuda
16. Vaccines against Vector-Borne Diseases
Chrysoula Kitsou and Utpal Pal
17. A quantum vaccinomics approach based on protein-protein interactions
Marinela Contreras, Sara Artigas- Jerónimo, Juan J. Pastor Comín, and José de la Fuente
18. Screening for the `Achilles Heel´ of Hyalomma anatolicum Ticks by RNA Interference Technology and an Update on anti-Tick Vaccine Design
Manjunathachar HV, Azhahianambi P, Binod Kumar, and Ghosh S
19. Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response
Kodai Kusakisako, Takeshi Miyata, Kozo Fujisaki, and Tetsuya Tanaka
20. Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction
Seham HM Hendawy, Heba F. Alzan, Tetsuya Tanaka, and Mona
This volume provides a practical guide providing step-by-step protocol to explore vaccines for farm and companion animals, as well as for fish and insects. Divided into three volumes, Volume 2: Vaccines for Veterinary Diseases guides readers through veterinary vaccines, vaccines for poultry, vaccines for farm animals, and vaccines for veterinary parasites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and practical,
Vaccine Design: Methods and Protocols, Second Edition, Volume 2: Vaccines for Veterinary Diseases aims to be a useful practical guide to researchers to help further their study in this field.
Challenges in Veterinary Vaccine Development.- Mammalian Cell Culture as a Platform for Veterinary Vaccines.- Avian Paramyxoviruses as Vectors for Vaccine Development.- Reverse Genetics and its Usage in the Development of Vaccine against Poultry Diseases.- Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies Paratuberculosis Vaccines.- Recombinant Escherichia coli Cell lysates as a low-cost alternative for vaccines against veterinary clostridial diseases.- Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli.- Macrophage Stimulation as a Useful Approach for Immunoscreening of Potential Vaccine Candidates against Toxoplasma gondii and Neospora caninum Infections.- Fundamentals of Fish Vaccination.- Teleost Fish as an Experimental Model for Vaccine Development.- Development of Nano-conjugated DNA Vaccine against Edwardsiellosis Diseasein Fish.- Development and Evaluation of DNA Vaccine against Salmonid Alphavirus.- Novel Vaccine Development for Fish Culture Based on the Multiepitope Concept.- Testing Novel Inactivation Methods and Adjuvants for Vaccines against Streptococcus Agalactiae in Nile Tilapia Oreochromis niloticus.- Anti-tick Vaccines: Current Advances and Future Prospects.- Vaccines against Vector-Borne Diseases.- A quantum vaccinomics approach based on protein-protein interactions.- Screening for the 'Achilles Heel' of Hyalomma anatolicum Ticks by RNA Interference Technology and an Update on anti-Tick Vaccine Design.- Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response.- Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction.
Inhaltsverzeichnis
PART I VETERINARY VACCINES: INTRODUCTION
1. Challenges in Veterinary Vaccine Development
Sunil Thomas, Ann Abraham, Alina Rodríguez-Mallon, Sasimanas Unajak, and John P. Bannantine
PART II TRENDS IN VETERINARY VACCINES
2. Mammalian Cell Culture as a Platform for Veterinary Vaccines
Thailín Lao González, Ileanet Ávalos Olivera, and Alina Rodríguez-Mallon
3. Avian Paramyxoviruses as Vectors for Vaccine Development
Shin-Hee Kim, Edris Shirvani, and Siba Samal
PART III VACCINES FOR POULTRY
4. Reverse Genetics and its Usage in the Development of Vaccine against Poultry Diseases
Barnali Nath, Sudhir Morla, and Sachin Kumar
PART IV VACCINES FOR FARM ANIMALS
5. Use of a Ferret Model to Test Efficacy and Immunogenicity of Live Attenuated Mycobacterium avium Subspecies Paratuberculosis Vaccines
John P. Bannantine, Tuhina Gupta, Denise K. Zinniel, Ahmed Hikal, Frederick D. Quinn, and Raul G. Barletta
6. Recombinant Escherichia coli Cell lysates as a low-cost alternative for vaccines against veterinary clostridial diseases
Clóvis Moreira Jr, Gustavo Marçal Schmidt Garcia Moreira, Rafael Amaral Donassolo, Marcos Roberto Alves Ferreira, Mariliana Luiza Ferreira Alves, Rafael Rodrigues Rodrigues, and Fabricio Rochedo Conceição
7. Clostridium spp. Toxins: A Practical Guide for Expression and Characterization in Escherichia coli
Mariliana Luiza Ferreira Alves, Gustavo Marçal Schmidt Garcia Moreira, Marcos Roberto Alves Ferreira, Rafael Amaral Donassolo1, Clóvis Moreira Jr, Rafael Rodrigues Rodrigues, and Fabricio Rochedo Conceição
PART V VACCINES FOR VETERINARY PARASITES
8. Macrophage Stimulation as a Useful Approach for Immunoscreening of Potential Vaccine Candidates against Toxoplasma gondii and Neospora caninum Infections
Ragab M. Fereig and Yoshifumi Nishikawa
PART VI VACCINES FOR FISH
9. Fundamentals of Fish Vaccination
Megha Kadam Bedekar and Sajal Kole
10. Teleost Fish as an Experimental Model for Vaccine Development
Marco Antonio de Andrade Belo and Ives Charlie-Silva
11. Development of Nano-conjugated DNA Vaccine against Edwardsiellosis Disease in Fish
Megha Kadam Bedekar and Sajal Kole
12. Development and Evaluation of DNA Vaccine against Salmonid Alphavirus
Chia-Jung Chang
13. Novel Vaccine Development for Fish Culture Based on the Multiepitope Concept
Sasimanas Unajak, Ansaya Pumchan, Sittiruk Roytrakul, Orathai Sawatdichaikul,
and Nontawith Areechon
14. Testing Novel Inactivation Methods and Adjuvants for Vaccines against Streptococcus Agalactiae in Nile Tilapia Oreochromis niloticus
Fernando Carlos Ramos-Espinoza, Victor Alexander Cueva-Quiroz, Jefferson Yunis-Aguinaga, Norquis Caled Alvarez-Rubio, Nicoli Paganoti de Mello, and Julieta Rodini Engrácia de Moraes
PART VII VACCINES AGAINST TICKS
15. Anti-tick Vaccines: Current Advances and Future Prospects
Dennis Muhanguzi, Christian Ndekezi, Joseph Nkamwesiga, Shewit Kalayou, Sylvester Ochwo, Moses Vuyani, and Magambo Phillip Kimuda
16. Vaccines against Vector-Borne Diseases
Chrysoula Kitsou and Utpal Pal
17. A quantum vaccinomics approach based on protein-protein interactions
Marinela Contreras, Sara Artigas- Jerónimo, Juan J. Pastor Comín, and José de la Fuente
18. Screening for the 'Achilles Heel' of Hyalomma anatolicum Ticks by RNA Interference Technology and an Update on anti-Tick Vaccine Design
Manjunathachar HV, Azhahianambi P, Binod Kumar, and Ghosh S
19. Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response
Kodai Kusakisako, Takeshi Miyata, Kozo Fujisaki, and Tetsuya Tanaka
20. Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction
Seham HM Hendawy, Heba F. Alzan, Tetsuya Tanaka, and Mona
Klappentext
This volume provides a practical guide providing step-by-step protocol to explore vaccines for farm and companion animals, as well as for fish and insects. Divided into three volumes, Volume 2: Vaccines for Veterinary Diseases guides readers through veterinary vaccines, vaccines for poultry, vaccines for farm animals, and vaccines for veterinary parasites. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.nnAuthoritative and practical, Vaccine Design: Methods and Protocols, Second Edition, Volume 2: Vaccines for Veterinary Diseases aims to be a useful practical guide to researchers to help further their study in this field.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts