Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.
Part I: Cell lines with Minimal but Inducible Proviral Expression
1. Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency
Anthony RODARI, Guido Poli, and Carine VAN LINT
2. U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency
Guido Poli
Part II: In vitro and ex vivo primary cell models of minimal but inducible proviral expression
3. An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency
in primary human CD4+ T cells
Rui Li and Fabio Romerio
4. The cultured TCM model of HIV latency
Alberto Bosque
5. A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) Method
Meenakshi Shukla, Fredrick Kizito, Uri Mbonye, Kien Nguyen, Curtis Dobrowolski, and Jonathan Karn
6. TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model
Sydney Bergstresser and Deanna A. Kulpa
7. Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoirs Quantification
Amélie Cattin, Augustine Fert, Delphine Planas, and Petronela Ancuta
8. Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF)
Claudia Alteri, Lorenzo Piermatteo, Francesca Ceccherini Silberstein, Valentina Svicher, and Carlo Federico Perno
9. Human monocyte-derived macrophages (MDM) - Model 2
Francesca Graziano, Elisa Vicenzi, and Guido Poli
10. Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100
Amelie Bauer
11. Hematopoietic stem and progenitor cells (HSPCs)
Valeri H. Terry, Gretchen E. Zimmerman, Maria C. Virgilio, Mark M. Painter, Dale Bixby, and Kathleen L. Collins
Part III: In vitro and ex vivo tissue-derived models of reversible proviral latency
12. HIV reservoirs: Modeling, Quantification and Approaches to a Cure
Louise A. Ouattara, Nikolas C. Vann, and Gustavo F. Doncel
13. More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication
Jean-Pierre Routy, Franck P. Dupuy, John Lin, and Stéphane Isnard
Part IV: Animal Models
14. Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells
Fernando Real, Yonatan Ganor, and Morgane Bomsel
15. Decidua basalis: An ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond
Nabila Jabrane-Ferrat and Hicham El Costa
16. HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure
Amir Dashti, Vidisha Singh, and Ann Chahroudi
17. Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research
Triana Rivera-Megias, Nhut M Le, and Alonso Heredia
18. Inducing long-term HIV-1 latency in the TKO-BLT mouse model
Yunyun Di and Kerry J. Lavender
Part V: Methods for detection and analysis of the reservoir
19. In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue
Kathleen Busman-Sahay, Michael D. Nekorchuk, Carly Elizabeth Starke, Chi Ngai Chan, and Jacob D. Estes
20. Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH
Mathieu Dubé and Daniel E. Kaufmann
21. Ex vivo differentiation of resting CD4+ T lymphocytes enhances detection of replication competent HIV-1 in viral outgrowth assays
Elizabeth R. Wonderlich, Monica D. Reece, and Deanna A. Kulpa
22. Quantitative Viral Outgrowth Assay to Measure the functional SIV Reservoir in Myeloid Cells
Abreu CM, Veenhuis RT, Shirk EN, Queen SE, Bullock BT, Mankowski JL, Gama L and Clements JE
23. Near-full-length single-genome HIV-1 DNA sequencing
Guinevere Q. Lee and Mathias Lichterfeld
24. TILDA: Tat/rev Induced Limiting Dilution Assay
Cynthia Lungu and Francesco A. Procopio
Part VI: Cure and long-term remission strategies
25. Latency Reversal and Clearance of Persistent HIV Infection
David M. Margolis
26. Cure and long-term remission strategies
Luisa Mori and Susana T. Valente
27. Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils for CRISPR Therapy
Jonathan Herskovitz, Mahmudul Hasan, Milankumar Patel, Bhavesh D Kevadiya,and Howard E. Gendelman
This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines,
in vitro and
ex vivo primary cell models of persistent infection,
in vitro and
ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful
Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.
This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.
Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency.- U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency.- An
In Vitro System to Model the Establishment and Reactivation of HIV-1 Latencyin primary human CD4+ T cells.- The cultured T
CM model of HIV latency.- A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) Method.- TGF-b signaling supports HIV latency in a Memory CD4+ T cell based
in vitro model.p Flow Cytometry Sorting of Memory CCR6
+CD4
+ T-Cells for HIV Reservoirs Quantification.- Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF).- Human monocyte-derived macrophages (MDM) - Model 2.- Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100.- Hematopoietic stem and progenitor cells (HSPCs).- HIV reservoirs: Modeling, Quantification and Approaches to a Cure.- More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication.- Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells.-
Decidua basalis: An
ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond.- HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure.- Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research.- Inducing long-term HIV-1 latency in the TKO-BLT mouse model.-
In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue.- Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH.-
Ex vivo differentiation of resting CD4+ T lymphocytes enhances detection of replication competent HIV-1 in viral outgrowth assays.- Quantitative Viral Outgrowth Assay to Measure the functional SIV Reservoir in Myeloid Cells.- Near-full-length single-genome HIV-1 DNA sequencing.- TILDA: Tat/rev Induced Limiting Dilution Assay.- Latency Reversal and Clearance of Persistent HIV Infection.- Cure and long-term remission strategies.- Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils for CRISPR Therapy.
Inhaltsverzeichnis
Part I: Cell lines with Minimal but Inducible Proviral Expression
1. Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency
Anthony RODARI, Guido Poli, and Carine VAN LINT
2. U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency
Guido Poli
Part II: In vitro and ex vivo primary cell models of minimal but inducible proviral expression
3. An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency
in primary human CD4+ T cells
Rui Li and Fabio Romerio
4. The cultured TCM model of HIV latency
Alberto Bosque
5. A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) Method
Meenakshi Shukla, Fredrick Kizito, Uri Mbonye, Kien Nguyen, Curtis Dobrowolski, and Jonathan Karn
6. TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model
Sydney Bergstresser and Deanna A. Kulpa
7. Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoirs Quantification
Amélie Cattin, Augustine Fert, Delphine Planas, and Petronela Ancuta
8. Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF)
Claudia Alteri, Lorenzo Piermatteo, Francesca Ceccherini Silberstein, Valentina Svicher, and Carlo Federico Perno
9. Human monocyte-derived macrophages (MDM) - Model 2
Francesca Graziano, Elisa Vicenzi, and Guido Poli
10. Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100
Amelie Bauer
11. Hematopoietic stem and progenitor cells (HSPCs)
Valeri H. Terry, Gretchen E. Zimmerman, Maria C. Virgilio, Mark M. Painter, Dale Bixby, and Kathleen L. Collins
Part III: In vitro and ex vivo tissue-derived models of reversible proviral latency
12. HIV reservoirs: Modeling, Quantification and Approaches to a Cure
Louise A. Ouattara, Nikolas C. Vann, and Gustavo F. Doncel
13. More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication
Jean-Pierre Routy, Franck P. Dupuy, John Lin, and Stéphane Isnard
Part IV: Animal Models
14. Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells
Fernando Real, Yonatan Ganor, and Morgane Bomsel
15. Decidua basalis: An ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond
Nabila Jabrane-Ferrat and Hicham El Costa
16. HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure
Amir Dashti, Vidisha Singh, and Ann Chahroudi
17. Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research
Triana Rivera-Megias, Nhut M Le, and Alonso Heredia
18. Inducing long-term HIV-1 latency in the TKO-BLT mouse model
Yunyun Di and Kerry J. Lavender
Part V: Methods for detection and analysis of the reservoir
19. In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue
Kathleen Busman-Sahay, Michael D. Nekorchuk, Carly Elizabeth Starke, Chi Ngai Chan, and Jacob D. Estes
20. Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH
Mathieu Dubé and Daniel E. Kaufmann
21. Ex vivo differentiation of resting CD4+ T lymphocytes enhances detection of replication competent HIV-1 in viral outgrowth assays
Elizabeth R. Wonderlich, Monica D. Reece, and Deanna A. Kulpa
22. Quantitative Viral Outgrowth Assay to Measure the functional SIV Reservoir in Myeloid Cells
Abreu CM, Veenhuis RT, Shirk EN, Queen SE, Bullock BT, Mankowski JL, Gama L and Clements JE
23. Near-full-length single-genome HIV-1 DNA sequencing
Guinevere Q. Lee and Mathias Lichterfeld
24. TILDA: Tat/rev Induced Limiting Dilution Assay
Cynthia Lungu and Francesco A. Procopio
Part VI: Cure and long-term remission strategies
25. Latency Reversal and Clearance of Persistent HIV Infection
David M. Margolis
26. Cure and long-term remission strategies
Luisa Mori and Susana T. Valente
27. Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils for CRISPR Therapy
Jonathan Herskovitz, Mahmudul Hasan, Milankumar Patel, Bhavesh D Kevadiya,and Howard E. Gendelman
Klappentext
This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
