Cutting-edge methods for genome-scale experiments and small-scale individual PCR amplifications
Highlights basic principles and software for PCR primer design
An exceptional reference for all those involved in studying the genome
At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate deillegalscriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
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In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as tranillegalscriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate deillegalscriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome.
In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists – geneticists, molecular biologists, molecular geneticists, and more – who routinely use PCR in their research.
PCR primer design. ContentsPart I. Basic Principles and software for PCR primer designChapter 1. Physical Principles and Visual-OMP Software for Optimal PCRDesignChapter 2. Oligo 7 Primer Analysis SoftwareChapter 3. Selection for 3' end triplets for polymerase chain reactionprimersChapter 4. The reference point method in primer designChapter 5. PCR primer design using statistical modelingChapter 6. Developing a Statistical Model for Primer DesignPart II. Genome scale PCR primer designChapter 7. GST-PRIME: An Algorithm for Genome-Wide Primer DesignChapter 8. Genome-Scale Probe and Primer Design with PRIMEGENSRepeat masking for PCR primer designChapter 9. SNPbox: web-based high-throughput primer design with an eyefor repetitive sequences.Chapter 10. Fast masking of repeated primer binding sites in eukaryoticgenomesMultiplex PCR primer designChapter 11. Degenerate Primer Design: Theoretical Analysis and theHYDEN programChapter 12. An Iterative Method for Selecting Degenerate Multiplex PCRPrimersChapter 13. Primer-design for multiplexed genotypingChapter 14. MultiPLX: automatic grouping and evaluation of PCR primersChapter 15. MultiPrimer: A system for Microarray PCR Primer DesignAllele-specific PCRChapter 16. Modified oligonucleotides as tools for allele-specificamplificationChapter 17. AlleleID: A Pathogen Detection and Identification SystemLong PCR primer designChapter 18. GenoFrag, a software package for the design of primersdedicated to whole genome scanning by LR-PCR.DNA methylation mappingChapter 19. Designing PCR primer for DNA methylation mappingChapter 20. BiSearch: ePCR tool for native or bisulfite treated genomictemplate.Chapter 21. Graphical design of primers with PerlPrimer
In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as tranillegalscriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate deillegalscriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome.
In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists - geneticists, molecular biologists, molecular geneticists, and more - who routinely use PCR in their research.
Basic Principles and Software for PCR Primer Design.- Physical Principles and Visual-OMP Software for Optimal PCR Design.- OLIGO 7 Primer Analysis Software.- Selection for 3?-End Triplets for Polymerase Chain Reaction Primers.- The Reference Point Method in Primer Design.- PCR Primer Design Using Statistical Modeling.- Developing a Statistical Model for Primer Design.- Genome-Scale PCR Primer Design.- GST-PRIME.- Genome-Scale Probe and Primer Design with PRIMEGENS.- Repeat Masking for PCR Primer Design.- SNPbox.- Fast Masking of Repeated Primer Binding Sites in Eukaryotic Genomes.- Multiplex PCR Primer Design.- Degenerate Primer Design.- An Iterative Method for Selecting Degenerate Multiplex PCR Primers.- Primer Design for Multiplexed Genotyping.- MultiPLX.- MultiPrimer.- Allele-specific PCR.- Modified Oligonucleotides as Tools for Allele-Specific Amplification.- AlleleID.- Long PCR Primer Design.- Designing Primers for Whole Genome PCR Scanning Using the Software Package GenoFrag.- DNA Methylation Mapping.- Designing PCR Primer for DNA Methylation Mapping.- BiSearch.- Graphical Design of Primers with PerlPrimer.
Inhaltsverzeichnis
PCR primer design. Contents
Part I. Basic Principles and software for PCR primer design
Chapter 1. Physical Principles and Visual-OMP Software for Optimal PCR
Design
Chapter 2. Oligo 7 Primer Analysis Software
Chapter 3. Selection for 3' end triplets for polymerase chain reaction
primers
Chapter 4. The reference point method in primer design
Chapter 5. PCR primer design using statistical modeling
Chapter 6. Developing a Statistical Model for Primer Design
Part II. Genome scale PCR primer design
Chapter 7. GST-PRIME: An Algorithm for Genome-Wide Primer Design
Chapter 8. Genome-Scale Probe and Primer Design with PRIMEGENS
Repeat masking for PCR primer design
Chapter 9. SNPbox: web-based high-throughput primer design with an eye
for repetitive sequences.
Chapter 10. Fast masking of repeated primer binding sites in eukaryotic
genomes
Multiplex PCR primer design
Chapter 11. Degenerate Primer Design: Theoretical Analysis and the
HYDEN program
Chapter 12. An Iterative Method for Selecting Degenerate Multiplex PCR
Primers
Chapter 13. Primer-design for multiplexed genotyping
Chapter 14. MultiPLX: automatic grouping and evaluation of PCR primers
Chapter 15. MultiPrimer: A system for Microarray PCR Primer Design
Allele-specific PCR
Chapter 16. Modified oligonucleotides as tools for allele-specific
amplification
Chapter 17. AlleleID: A Pathogen Detection and Identification System
Long PCR primer design
Chapter 18. GenoFrag, a software package for the design of primers
dedicated to whole genome scanning by LR-PCR.
DNA methylation mapping
Chapter 19. Designing PCR primer for DNA methylation mapping
Chapter 20. BiSearch: ePCR tool for native or bisulfite treated genomic
template.
Chapter 21. Graphical design of primers with PerlPrimer
Klappentext
At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). This book focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate deillegalscriptions of basic approaches as well as specialized methods, "PCR Primer Design" is an exceptional reference for all those involved in studying the genome.
Cutting-edge methods for genome-scale experiments and small-scale individual PCR amplificationsnHighlights basic principles and software for PCR primer designnAn exceptional reference for all those involved in studying the genome
