A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.
Forensic DNA-Typing Technologies: A Review Angel Carracedo and Paula Sánchez-Diz DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR Silvano Köchl, Harald Niederstätter, and Walther Parson A Real-Time PCR Protocol to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA Samples Antonio Alonso and Pablo Martín Species Determination: The Role and Use of the Cytochrome b Gene Adrian Linacre and James Chun-I Lee Constructing STR Mulitplex Assays John M. Butler Y Chromosome STR Typing Leonor Gusmão and Cíntia Alves Using Online Databases for Developing SNP Markers of Forensic Interest Christopher Phillips SNP Typing in Forensic Genetics: A Review Beatriz Sobrino and Angel Carracedo Genotyping SNPs With the LightCycler María Victoria Lareu and Clara Ruiz-Ponte Universal Tag Arrays in Forensic SNP Analysis Marie Allen and Anna-Maria Divne SNP Typing on the NanoChip Electronic Microarray Claus Børsting, Juan J. Sanchez, and Niels Morling Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS Peter M. Vallone, Kristina Fahr, and Markus Kostrzewa Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing Marie Allen and Hanna Andréasson SNaPshot Typing of Mitochondrial DNA Coding Region Variants Antonio Salas, Beatriz Quintáns, and Vanesa Álvarez-Iglesias Typing of Y Chromosome SNPs With Multiplex PCR Methods Juan J. Sanchez, Claus Børsting, and Niels Morling Y Chromosome SNP Analysis Using Single-Base Extension: A Hierarchical Multiplex Design María Brión SNaPshot for Pharmacogenetics by Minisequencing Klaus Bender Methods for the Study of Ancient DNA Christine Keyser-Tracqui and BertrandLudes Protocols for Ancient DNATyping Cristian Capelli and Frank Tschentscher Index
In recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science.
A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs. In recent years, the rapid emergence of different markers and technologies for DNA typing has transformed the field of forensic identification with breathtaking speed. In Forensic DNA Typing Protocols, leading forensic geneticists from around the world describe in step-by-step detail the cutting-edge laboratory methods they now use for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. Additional chapters address new applications in the forensic genetics lab, such a species identification, ancient DNA, or typing of CYP polymorphisms for the analysis of adverse to drugs. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Forensic DNA Typing Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science.
Forensic DNA-Typing Technologies.- DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR.- A Real-Time PCR Protocol to Determine the Number of Amelogenin (X-Y) Gene Copies From Forensic DNA Samples.- Species Determination.- Constructing STR Multiplex Assays.- Y Chromosome STR Typing.- Using Online Databases for Developing SNP Markers of Forensic Interest.- SNP Typing in Forensic Genetics.- Genotyping SNPs With the LightCycler.- Universal Tag Arrays in Forensic SNP Analysis.- SNP Typing on the NanoChip Electronic Microarray.- Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS.- Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing.- SNaPshot Typing of Mitochondrial DNA Coding Region Variants.- Typing of Y Chromosome SNPs With Multiplex PCR Methods.- Y Chromosome SNP Analysis Using the Single-Base Extension.- SNaPshot for Pharmacogenetics by Minisequencing.- Methods for the Study of Ancient DNA.- Protocols for Ancient DNA Typing.
Inhaltsverzeichnis
Forensic DNA-Typing Technologies: A Review
Angel Carracedo and Paula Sánchez-Diz
DNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCR
Silvano Köchl, Harald Niederstätter, and Walther Parson
A Real-Time PCR Protocol to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA Samples
Antonio Alonso and Pablo Martín
Species Determination: The Role and Use of the Cytochrome b Gene
Adrian Linacre and James Chun-I Lee
Constructing STR Mulitplex Assays
John M. Butler
Y Chromosome STR Typing
Leonor Gusmão and Cíntia Alves
Using Online Databases for Developing SNP Markers of Forensic Interest
Christopher Phillips
SNP Typing in Forensic Genetics: A Review
Beatriz Sobrino and Angel Carracedo
Genotyping SNPs With the LightCycler
María Victoria Lareu and Clara Ruiz-Ponte
Universal Tag Arrays in Forensic SNP Analysis
Marie Allen and Anna-Maria Divne
SNP Typing on the NanoChip Electronic Microarray
Claus Børsting, Juan J. Sanchez, and Niels Morling
Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS
Peter M. Vallone, Kristina Fahr, and Markus Kostrzewa
Mitochondrial D-Loop and Coding Sequence Analysis Using Pyrosequencing
Marie Allen and Hanna Andréasson
SNaPshot Typing of Mitochondrial DNA Coding Region Variants
Antonio Salas, Beatriz Quintáns, and Vanesa Álvarez-Iglesias
Typing of Y Chromosome SNPs With Multiplex PCR Methods
Juan J. Sanchez, Claus Børsting, and Niels Morling
Y Chromosome SNP Analysis Using Single-Base Extension: A Hierarchical Multiplex Design
María Brión
SNaPshot for Pharmacogenetics by Minisequencing
Klaus Bender
Methods for the Study of Ancient DNA
Christine Keyser-Tracqui and BertrandLudes
Protocols for Ancient DNATyping
Cristian Capelli and Frank Tschentscher
Index
Klappentext
A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.
Includes supplementary material: sn.pub/extras
