Includes supplementary material: sn.pub/extras
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Establishment and Maintenance of Normal Human Keratinocyte Cultures Claire Linge Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters Mei-Yu Hsu, Ling Li, and Meenhard Herlyn Isolation and Culture of Human Osteoblasts Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, and James A. Gallagher Human Osteoclast Culture from Peripheral Blood Monocytes: Phenotypic Characterization and Quantitation of Resorption Katherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, and James A. Gallager Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation Mary B. Goldring Human Myoblasts and Muscle-Derived SP Cells Grace K. Pavlath and Emanuela Gussoni Cell Cultures of Autopsy-Derived Fibroblasts Volker Meske, Frank Albert, and Thomas G. Ohm Primary Culture and Differentiation of Human Adipocyte Precursor Cells Vanessa van Harmelen, Thomas Skurk, and Hans Hauner Human Mononuclear Phagocytes in Tissue Culture Yona Keisari Purification of Peripheral Blood Natural Killer Cells Bice Perussia and Matthew J. Loza Human Fetal Brain Cell Culture Mark P. Mattson Culturing Human Schwann Cells Victor J. Turnbull Well-Differentiated Human Airway Epithelial Cell Cultures M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. Yankaskas, and Scott H. Randell Isolation and Culture of Human Alveolar Epithelial Cells Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr A New Approach to Primary Culture of Human Gastric Epithelium Pierre Chailler and Daniel Ménard Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach Hamid A. Mohammadpour Isolation and Culture of HumanHepatocytes Martin Bayliss and Graham Somers Glomerular Epithelial and Mesangial Cell Culture and Characterization Heather M. Wilson and Keith N. Stewart Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules Gabrielle M. Hawksworth Culture of Parathyroid Cells Per Hellman Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media Daniel W. Fraga, A. Osama Gaber, and Malak Kotb Primary Culture of Human Antral Endocrine and Epithelial Cells Susan B. Curtis and Alison M. J. Buchan Conjunctiva Organ and Cell Culture Monica Berry and Marcus Radburn-Smith Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman, Monte A. DelMonte, and Douglas A. Greene Index
In this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.
"This book offers a welcome text which is value to anybody undertaking human cell culture." - Cell Biochemistry and Function
"...a very useful general text for researchers involved in human cell cultures. -Immunology News
From Reviews of the First Edition...
"...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information." -Trends in Cell Biology
"...an invaluable and highly recommended resource for cell culturists setting up new systems." -Molecular Biotechnology
"...will be of significance to a very wide range of scientists." -ISSX Newsletter
"...has a wealth of down-to-earth, ready-to-use information." -Modern Pathology
"..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered." -BTS Newsletter
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Establishment and Maintenance of Normal Human Keratinocyte Cultures.- Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters.- Isolation and Culture of Human Osteoblasts.- Human Osteoclast Culture From Peripheral Blood Monocytes.- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation.- Human Myoblasts and Muscle-Derived SP Cells.- Cell Cultures of Autopsy-Derived Fibroblasts.- Primary Culture and Differentiation of Human Adipocyte Precursor Cells.- Human Mononuclear Phagocytes in Tissue Culture.- Purification of Peripheral Blood Natural Killer Cells.- Human Fetal Brain Cell Culture.- Culturing Human Schwann Cells.- Well-Differentiated Human Airway Epithelial Cell Cultures.- Isolation and Culture of Human Alveolar Epithelial Cells.- A New Approach to Primary Culture of Human Gastric Epithelium.- Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach.- Isolation and Culture of Human Hepatocytes.- Glomerular Epithelial and Mesangial Cell Culture and Characterization.- Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules.- Culture of Parathyroid Cells.- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media.- Primary Culture of Human Antral Endocrine and Epithelial Cells.- Conjunctiva Organ and Cell Culture.- Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium.
"This book offers a welcome text which is value to anybody undertaking human cell culture." - Cell Biochemistry and Function
"...a very useful general text for researchers involved in human cell cultures. -Immunology News
From Reviews of the First Edition...
"...packed full of methods for the culture of cells from human tissues...an excellent book full of very useful information." -Trends in Cell Biology
"...an invaluable and highly recommended resource for cell culturists setting up new systems." -Molecular Biotechnology
"...will be of significance to a very wide range of scientists." -ISSX Newsletter
"...has a wealth of down-to-earth, ready-to-use information." -Modern Pathology
"..a valuable reference work for workers looking for detailed methodology for culturing the specific cell types covered." -BTS Newsletter
Inhaltsverzeichnis
Establishment and Maintenance of Normal Human Keratinocyte Cultures
Claire Linge
Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters
Mei-Yu Hsu, Ling Li, and Meenhard Herlyn
Isolation and Culture of Human Osteoblasts
Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, and James A. Gallagher
Human Osteoclast Culture from Peripheral Blood Monocytes: Phenotypic Characterization and Quantitation of Resorption
Katherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, and James A. Gallager
Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Mary B. Goldring
Human Myoblasts and Muscle-Derived SP Cells
Grace K. Pavlath and Emanuela Gussoni
Cell Cultures of Autopsy-Derived Fibroblasts
Volker Meske, Frank Albert, and Thomas G. Ohm
Primary Culture and Differentiation of Human Adipocyte Precursor Cells
Vanessa van Harmelen, Thomas Skurk, and Hans Hauner
Human Mononuclear Phagocytes in Tissue Culture
Yona Keisari
Purification of Peripheral Blood Natural Killer Cells
Bice Perussia and Matthew J. Loza
Human Fetal Brain Cell Culture
Mark P. Mattson
Culturing Human Schwann Cells
Victor J. Turnbull
Well-Differentiated Human Airway Epithelial Cell Cultures
M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. Yankaskas, and Scott H. Randell
Isolation and Culture of Human Alveolar Epithelial Cells
Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr
A New Approach to Primary Culture of Human Gastric Epithelium
Pierre Chailler and Daniel Ménard
Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach
Hamid A. Mohammadpour
Isolation and Culture of HumanHepatocytes
Martin Bayliss and Graham Somers
Glomerular Epithelial and Mesangial Cell Culture and Characterization
Heather M. Wilson and Keith N. Stewart
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
Gabrielle M. Hawksworth
Culture of Parathyroid Cells
Per Hellman
Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media
Daniel W. Fraga, A. Osama Gaber, and Malak Kotb
Primary Culture of Human Antral Endocrine and Epithelial Cells
Susan B. Curtis and Alison M. J. Buchan
Conjunctiva Organ and Cell Culture
Monica Berry and Marcus Radburn-Smith
Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium
Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman, Monte A. DelMonte, and Douglas A. Greene
Index
Klappentext
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Includes supplementary material: sn.pub/extras
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