Includes supplementary material: sn.pub/extras
This volume of the Methods in Molecular Biology series is entirely devoted to the study of steroid receptor biology. Steroid hormone receptors represent a powerful system for the study of both the most fundamental molecular mec- nisms of gene regulation and control and the gross physiological responses of organisms to steroid hormones. Research in this field has brought forth advances in the treatment of cancer, endocrine disorders, and reproductive biology, and allowed elucidation of the fundamental biological mechanisms of gene expr- sion. In Steroid Receptor Methods: Protocols and Assays, the reader will find a collection of methods and protocols submitted by many fine steroid receptor researchers from throughout the world. These authors have been instructed to create a highly informative cross-section of the latest research techniques ava- able. The resulting work is timely, useful, and approachable for both the ex- rienced researcher and the novice to the field. Because the steroid receptor family is represented by a wonderfully diverse, yet strongly interrelated set of steroid receptor proteins, Steroid Receptor Methods contains protocols for the prod- tion and purification of a variety of receptor forms, including the progesterone, glucocorticoid, and androgen receptors. These procedures provide the raw ma- rial needed to conduct sophisticated biochemical analysis of receptor properties. Other techniques presented allow the reader to perform biochemical experiments on DNA binding characteristics, hormone binding assays, and protocols using combinatorial chemistry for drug discovery.
Part I. Bioinformatics Bioinformatics of Nuclear Receptors Mark Danielsen Phylogenetic Inference and Parsimony Analysis Llewellyn D. Densmore III Part II. Purification Protocols Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems Vida Senkus Melvin and Dean P. Edwards High-Yield Purification of Functionally Active Glucocorticoid Receptor Terace M. Fletcher, Barbour S. Warren, Christopher T. Baumann, and Gordon L. Hager Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor Mingmin Liao and Elizabeth M. Wilson Large Scale Production of Nuclear Receptor Ligand-Binding Domains Li-Zhi Mi and Fraydoon Rastinejad Physical Structure of Nuclear Receptor-DNA Complexes Scott A. Chasse and Fraydoon Rastinejad Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display Sushma Kumar, Maarit Angervo, Milan K. Bagchi, and Indrani C. Bagchi Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes Bruce Blumberg Part III. Steroid Hormone-Binding Assays Use of [99mTc] Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors Frank Wüst Steroid Hormone Metabolites and Hormone Binding Assays Rosemary Bland and Martin Hewison Vitamin D3 Analog Screening Sami Väisänen, Sanna Ryhänen, and Pekka H. Mäenpää Part IV. Protein Interaction Assays Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors Steven K. Nordeen, Paul R. Housley, Yihong Wan, and Richard N. Day Knockout Mice and Steroid Receptor Research Per Flodby, Stephan Teglund, andJan-Åke Gustafsson Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors Bertrand Le Douarin, David M. Heery, Claudine Gaudon, Elmar vom Baur, and Régine Losson Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex Rabindra N. Bhattacharjee, Caroline Underhill, and Joseph Torchia Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System Simon J. Evans and Frank L. Moore Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay James R. Lambert and Steven K. Nordeen Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Tranillegalscription In Vivo Christy J. Fryer and Trevor K. Archer Cotransfection Assays and Steroid Receptor Biology Shimin Zhang and Mark Danielsen Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System Hironobu Sasano, Sachiko Matsuzaki, and Takashi Suzuki Part V. Cancer Research and Drug Discovery Solid Tumor Cancer Markers and Applications to Steroid Hormone Research Marcia V. Fournier, Katherine J. Martin, and Arthur B. Pardee Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay Kathleen F. Arcaro and John F. Gierthy Combinatorial Chemistry in Steroid Receptor Drug Discovery John A. Flygare, Daniel P. Sutherlin, and S. David Brown Identification of Nuclear Receptor Interacting Proteins Using Yeast Two-Hybrid Technology: Applications to Drug Discovery Sunil Nagpal, Corine R. Ghosn, and Roshantha A. S. Chandraratna
Research into steroid receptor biology holds great promise for the treatment of cancer and endocrine disorders and for elucidating the fundamental biological mechanisms of gene expression. In Steroid Receptor Methods: Protocols and Assays, a distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA-binding characteristics and hormone-binding assays and with the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness. Finally, a discussion by example presents the most current bioinformatics tools and analytical techniques.
Timely and highly accessible, Steroid Receptor Methods: Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery.
"This extremely well written and useful collection of laboratory methods for the study of steroid biology is one of a series in Methods in Molecular Biology devoted to steroid hormones and their receptors. The book provides an up-to-date compilation of protocols and assays that are important for the study of steroids and their receptors... it will be invaluable to researchers already in the field to compare protocols and improve methods...The book is divided into sections of bioinformatics, purification protocols, steroid hormone binding assays, protein interaction assays, and cancer research and drug discovery. The bioinformatics section leads off the book with a wealth of information on web sites that are relevant for steroid hormones and their receptors. While there are probably over 40 different proteins involved in steroid hormone receptor interaction, the section on protein interactions is superb. It actually clarifies a good part of the literature as so many of the proteins have different names for the same molecule. Understanding the mechanisms of isolation of these proteins puts the literature into perspective. Furthermore, the protocols are in sufficient detail that our lab was able to adapt several of the techniques immediately." -Doody's Health Sciences Book Review Journal
"This volume, part of the series 'Methods in Molecular Biology', describes in detail the protocols and assays used in the study of steroid hormone receptors." - Journal of Pediatric Endocrinology and Metabolism
"Timely and highly accessible, Steroid Receptor Methods. Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery." - Biomedicine & Pharmacotherapy
"Anyone working techniques, or who is tempted to enter the field, would be well advised to obtain this book and read the relevant chapters first. PhD student and established scientist alike will find Steroid Receptor Methods a valuable addition the the library." - Molecular Biotechnology
"Overall, the book is well written by an impressive list of experts and represents a valuable resource for both inexperienced and seasoned researchers of steroid receptors." -Today's Life Science
Bioinformatics.- Bioinformatics of Nuclear Receptors.- Phylogenetic Inference and Parsimony Analysis.- Purification Protocols.- Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems.- High-Yield Purification of Functionally Active Glucocorticoid Receptor.- Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor.- Large Scale Production of Nuclear Receptor Ligand-Binding Domains.- Physical Structure of Nuclear Receptor-DNA Complexes.- Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display.- Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes.- Steroid Hormone-Binding Assays.- Use of [99mTc]Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors.- Steroid Hormone Metabolites and Hormone Binding Assays.- Vitamin D3 Analog Screening.- Protein Interaction Assays.- Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors.- Knockout Mice and Steroid Receptor Research.- Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors.- Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex.- Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System.- Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay.- Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Tranillegalscription In Vivo.- Cotransfection Assays and Steroid Receptor Biology.- Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System.- Cancer Research and Drug Discovery.- Solid Tumor Cancer Markers and Applications to Steroid Hormone Research.- Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay.- Combinatorial Chemistry in Steroid Receptor Drug Discovery.- Identification of Nuclear Receptor Interacting Proteins Using Yeast Two- Hybrid Technology.
"This extremely well written and useful collection of laboratory methods for the study of steroid biology is one of a series in Methods in Molecular Biology devoted to steroid hormones and their receptors. The book provides an up-to-date compilation of protocols and assays that are important for the study of steroids and their receptors... it will be invaluable to researchers already in the field to compare protocols and improve methods...The book is divided into sections of bioinformatics, purification protocols, steroid hormone binding assays, protein interaction assays, and cancer research and drug discovery. The bioinformatics section leads off the book with a wealth of information on web sites that are relevant for steroid hormones and their receptors. While there are probably over 40 different proteins involved in steroid hormone receptor interaction, the section on protein interactions is superb. It actually clarifies a good part of the literature as so many of the proteins have different names for the same molecule. Understanding the mechanisms of isolation of these proteins puts the literature into perspective. Furthermore, the protocols are in sufficient detail that our lab was able to adapt several of the techniques immediately." -Doody's Health Sciences Book Review Journal
"This volume, part of the series 'Methods in Molecular Biology', describes in detail the protocols and assays used in the study of steroid hormone receptors." - Journal of Pediatric Endocrinology and Metabolism
"Timely and highly accessible, Steroid Receptor Methods. Protocols and Assays provides biochemists and molecular biologists studying endocrine systems with a full range of the powerful tools needed for elucidating protein interactions with receptors and DNA, as well as for drug discovery." - Biomedicine & Pharmacotherapy
"Anyone working techniques, or who is tempted to enter the field, would be well advised to obtain this book and read the relevant chapters first. PhD student and established scientist alike will find Steroid Receptor Methods a valuable addition the the library." - Molecular Biotechnology
"Overall, the book is well written by an impressive list of experts and represents a valuable resource for both inexperienced and seasoned researchers of steroid receptors." -Today's Life Science
Inhaltsverzeichnis
Part I. Bioinformatics
Bioinformatics of Nuclear Receptors
Mark Danielsen
Phylogenetic Inference and Parsimony Analysis
Llewellyn D. Densmore III
Part II. Purification Protocols
Expression and Purification of Recombinant Human Progesterone Receptor in Baculovirus and Bacterial Systems
Vida Senkus Melvin and Dean P. Edwards
High-Yield Purification of Functionally Active Glucocorticoid Receptor
Terace M. Fletcher, Barbour S. Warren, Christopher T. Baumann, and Gordon L. Hager
Production and Purification of Histidine-Tagged Dihydrotestosterone-Bound Full-Length Human Androgen Receptor
Mingmin Liao and Elizabeth M. Wilson
Large Scale Production of Nuclear Receptor Ligand-Binding Domains
Li-Zhi Mi and Fraydoon Rastinejad
Physical Structure of Nuclear Receptor-DNA Complexes
Scott A. Chasse and Fraydoon Rastinejad
Isolation of Steroid-Regulated Genes from the Uterus by mRNA Differential Display
Sushma Kumar, Maarit Angervo, Milan K. Bagchi, and Indrani C. Bagchi
Identification of Nuclear Hormone Receptor Homologs by Screening Libraries with Highly Degenerate Oligonucleotide Probes
Bruce Blumberg
Part III. Steroid Hormone-Binding Assays
Use of [99mTc] Technetium-Labeled Steroids as Probes for Steroid Hormone Receptors
Frank Wüst
Steroid Hormone Metabolites and Hormone Binding Assays
Rosemary Bland and Martin Hewison
Vitamin D3 Analog Screening
Sami Väisänen, Sanna Ryhänen, and Pekka H. Mäenpää
Part IV. Protein Interaction Assays
Application of Green Fluorescent Protein to the Study of Dynamic Protein-Protein Interactions and Subcellular Trafficking of Steroid Receptors
Steven K. Nordeen, Paul R. Housley, Yihong Wan, and Richard N. Day
Knockout Mice and Steroid Receptor Research
Per Flodby, Stephan Teglund, andJan-Åke Gustafsson
Yeast Two-Hybrid Screening for Proteins that Interact with Nuclear Hormone Receptors
Bertrand Le Douarin, David M. Heery, Claudine Gaudon, Elmar vom Baur, and Régine Losson
Isolation of a p300/CBP Cointegrator-Associated Protein Coactivator Complex
Rabindra N. Bhattacharjee, Caroline Underhill, and Joseph Torchia
Nonradioactive Photoaffinity Labeling of Steroid Receptors Using Western Blot Detection System
Simon J. Evans and Frank L. Moore
Analysis of Steroid Hormone-Induced Histone Acetylation by Chromatin Immunoprecipitation Assay
James R. Lambert and Steven K. Nordeen
Analyzing the Contributions of Chromatin Structure in Nuclear Hormone Receptor Activated Tranillegalscription In Vivo
Christy J. Fryer and Trevor K. Archer
Cotransfection Assays and Steroid Receptor Biology
Shimin Zhang and Mark Danielsen
Estrogen Receptor mRNA In Situ Hybridization Using Microprobe System
Hironobu Sasano, Sachiko Matsuzaki, and Takashi Suzuki
Part V. Cancer Research and Drug Discovery
Solid Tumor Cancer Markers and Applications to Steroid Hormone Research
Marcia V. Fournier, Katherine J. Martin, and Arthur B. Pardee
Assessing Modulation of Estrogenic Activity of Environmental and Pharmaceutical Compounds Using MCF-7 Focus Assay
Kathleen F. Arcaro and John F. Gierthy
Combinatorial Chemistry in Steroid Receptor Drug Discovery
John A. Flygare, Daniel P. Sutherlin, and S. David Brown
Identification of Nuclear Receptor Interacting Proteins Using Yeast Two-Hybrid Technology: Applications to Drug Discovery
Sunil Nagpal, Corine R. Ghosn, and Roshantha A. S. Chandraratna
Klappentext
A distinguished team of principal investigators and their associates describe in step-by-step detail a cross-section of the latest research techniques available for studying the endocrine system. As a basis for sophisticated biochemical analysis of receptor properties, the contributors provide methods for the production and purification of a variety of receptors, including progesterone, glucocorticoid, and androgen. Other protocols allow the reader to experiment with DNA binding characteristics, hormone binding assays, and the use of combinatorial chemistry for drug discovery. A series of novel methods utilizing the latest advances in immunochemistry, yeast two-hybrid screening, and fluorescence are included for the detection and analysis of a variety of cellular proteins that influence steroid receptor effectiveness.
Includes supplementary material: sn.pub/extras
