Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
JIAN CAORoom 004, Life Sciences BuildingDivision of Cancer PreventionDepartment of MedicineSchool of Medicine,Stony Brook UniversityStony Brook, NY 11794-5200 631-632-1815email: Jian.Cao@stonybrookmedicine.edu
This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer). Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.
Part I Detection of Molecular Markers of Breast Cancer
1. Basic Histopathological Methods and Breast Lesion Types for Research
Nengtai Ouyang and Lin Wang
2. Clinical Applications for Immunohistochemistry of Breast Lesions
Kester Haye, Rajarsi Gupta, Christopher Metter, and Jingxuan Liu
3. Immunohistochemistry for Triple-Negative Breast Cancer
Kalnisha Naidoo and Sarah E.
Pinder
4.
In Situ Hybridization of Breast Cancer Markers
Li Min and Chengchao Shou
5. Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin Embedded (FFPE) Tissue Specimens by Fluorescence
In Situ Hybridization (FISH)
Harry C. Hwang and Allen M. Gown
Part II Genetic Detection for Breast Cancer
6.
Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR)
Yiyi Li, Kai Wang, Longhua Chen, Xiaoxia Zhu, and Jie Zhou
7. Detection of MiRNA in Cultured Cells or Xenograft Tissues of Breast Cancer
Martin Brown and Meiyun Fan
8. Pyrosequencing Analysis for Breast Cancer DNA Methylome
Cem Kuscu and Canan Kuscu
Part III Isolation of Breast Cancer Cells
9. Vita-Assay ™ Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs)
Shaun Tulley, Qiang Zhao, Huan Dong, Michael L. Pearl, and Wen-Tien Chen
10. Breast Cancer Stem Cell Isolation
Xuanmao Jiao, Albert A. Rizvanov, Massimo Cristofanilli, Regina R. Miftakhova, and Richard G. Pestell
Part IV In Vitro Experimental Models for Breast Cancer
11. Cellular Apoptosis Assay of Breast Cancer
Yu Sun and Wei-Xing Zong
12. Assessment of Matrix Metalloproteinases by Gel Zymography
Jillian Cathcart
13. Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay
Ty J. Lively, Dale B. Bosco, Zahraa I. Khamis, and Qing-Xiang Amy Sang
14. Determination of Breast Cancer Cell Migratory Ability
David Schmitt, Joel Andrews, and Ming Tan
15. A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration
Vincent M. Alford, Eric Roth, Qian Zhang, and Jian Cao
16. Three-Dimensional Assay for Studying Breast Cancer Cell Invasion
Nikki A. Evensen
17. A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation
Ashleigh Pulkoski-Gross
18. Analysis of Invadopodia Formation in Breast Cancer Cells
Ziqing Wang, Xiao Liang, Ming Cai, and Guangwei Du
19. Patient-Derived Tumor Xenograft Models of Breast Cancer
Christopher D. Suarez and Laurie E. Littlepage
20. Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors
Maryia Lu Li, Wei Rachel Tay, Jingquan He, and Guangwei Du
21. 3D
In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method
Fransisca Leonard and Biana Godin
V In Vivo Experimental Models for Breast Cancer
22. Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model
Jacquelyn J. Ames, Terry Henderson, Lucy Liaw, and Peter C. Brooks
23. Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models
Wei Wang, Subhasree Nag, and Ruiwen Zhang
24. Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells
Derek Rammelkamp, Weiyi Li, and Yizhi Meng
25. Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression
Gregg B. Fields and Maciej J. Stawikowski
This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer).
Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough,
Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.
Basic Histopathological Methods and Breast Lesion Types for Research.- Clinical Applications for Immunohistochemistry of Breast Lesions.- Immunohistochemistry for Triple-Negative Breast Cancer.- In Situ Hybridization of Breast Cancer Markers.- Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH).- Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR).- Detection of MiRNA in Cultured Cells or Xenograft Tissues of Breast Cancer.- Pyrosequencin
g Analysis for Breast Cancer DNA Methylome.- Vita-Assay (TM) Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).- Breast Cancer Stem Cell Isolation.- Cellular Apoptosis Assay of Breast Cancer.- Assessment of Matrix Metalloproteinases by Gel Zymography.- Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay.- Determination of Breast Cancer Cell Migratory Ability.- A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration.- Three-Dimensional Assay for Studying Breast Cancer Cell Invasion.- A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation.- Analysis of Invadopodia Formation in Breast Cancer Cells.- Patient-Derived Tumor Xenograft Models of Breast Cancer.- Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors.- 3D
In Vitro Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method.- Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model.- Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models.- Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells.- Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.
Über den Autor
Inhaltsverzeichnis
Basic Histopathological Methods and Breast Lesion Types for Research.- Clinical Applications for Immunohistochemistry of Breast Lesions.- Immunohistochemistry for Triple-Negative Breast Cancer.-
In Situ
Hybridization of Breast Cancer Markers.- Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin Embedded (FFPE) Tissue Specimens by Fluorescence
In Situ
Hybridization (FISH).- Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR).- Detection of MiRNA in Cultured Cells or Xenograft Tissues of Breast Cancer.- Pyrosequencin
g Analysis for Breast Cancer DNA Methylome.- Vita-Assay (TM) Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs).- Breast Cancer Stem Cell Isolation.- Cellular Apoptosis Assay of Breast Cancer.- Assessment of Matrix Metalloproteinases by Gel Zymography.- Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay.- Determination of Breast Cancer Cell Migratory Ability.- A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration.- Three-Dimensional Assay for Studying Breast Cancer Cell Invasion.- A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation.- Analysis of Invadopodia Formation in Breast Cancer Cells.- Patient-Derived Tumor Xenograft Models of Breast Cancer.- Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors.- 3D
In Vitro
Model for Breast Cancer Research Using Magnetic Levitation and Bioprinting Method.- Methods for Analyzing Tumor Angiogenesis in the Chick Chorioallantoic Membrane Model.- Pharmacokinetics and Pharmacodynamics in Breast Cancer Animal Models.- Intracellular Delivery of Fluorescently Labeled Polysaccharide Nanoparticles to Cultured Breast Cancer Cells.- Imaging Matrix Metalloproteinase Activity Implicated in Breast Cancer Progression.
Klappentext
This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer). Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nnPractical and thorough, Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
