Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
Preface...
Table of Contents...
Contributing Authors...
1.Multi-Parametric Screening in Arabidopsis thaliana Seedlings
Long Nguyen, Andrzej Drozdzecki, Vera Goossens, Bert De Rybel, Tom Beeckman, and Dominique Audenaert
2.Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress
Susan Fozard and Brian G. Forde
3.Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen
Elena Marusich, Roman Chuprov-Netochin, Yaroslav Neskorodov, Yana Mishutkina, Polina Volynchuk, Sergey Leonov, Yan Ivanenkov, Alisher Touraev, and Klaus Palme
4.A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana
Yoshiteru Noutoshi and Ken Shirasu
5.High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression
Vivek Halder and Erich Kombrink
6.Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics
Clémence Bonnot, Laurent Nussaume, and Thierry Desnos
7.Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells
Emiko Okubo-Kurihara and Minami Matsui
8.Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes
Mitsuhiro Kimura and Sachiko Isobe
9.High-Throughput In Vitro Screening for Inhibitors of Cereal ?-glucosidase
Michael D. Rugen, Martin Rejzek, Henrik Naested, Birte Svensson, and Robert A. Field
10.Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana
Duncan Holbrook-Smith and Peter McCourt
11.Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress
Masanori Okamoto and Sean R. Cutler
12.Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening
Yajin Ye and Yang Zhao
13.Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman
Yousuke Takaoka and Minoru Ueda
14.Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule
Yoichi Takakusagi, Kaori Takakusagi, Fumio Sugawara, and Kengo Sakaguchi
15.Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH
Satria A. Kautsar, Hernando G. Suarez Duran, and Marnix H. Medema
16.Chemical Genomics Translatability from Unicellular to Multicellular Models
Cecilia Rodriguez-Furlán, Carlos Rubilar-Hernández, and Lorena Norambuena
17.Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii
Brittany Jack and Prachee Avasthi
18.FnCpf1-Mediated Targeted Mutagenesis in Plants
Akira Endo and Seiichi Toki
This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as
Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge,
Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
Multi-Parametric Screening in Arabidopsis thaliana Seedlings.- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress.- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen.- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana.- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression.- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics.- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells.- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes.- High-Throughput In Vitro Screeningfor Inhibitors of Cereal -glucosidase.- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana.- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress.- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening.- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman.- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule.- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH.- Chemical Genomics Translatability from Unicellular to Multicellular Models.- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii.- FnCpf1-Mediated Targeted Mutagenesis in Plants.
Inhaltsverzeichnis
Multi-Parametric Screening in Arabidopsis thaliana Seedlings.- Novel Micro-Phenotyping Approach to Chemical Genetic Screening for Increased Plant Tolerance to Abiotic Stress.- Method for Ultra-Rapid High-Content Screening for Biologically Active Chemicals Using Plant Pollen.- A High-Throughput Chemical Screening Method for Inhibitors and Potentiators of Hypersensitive Cell Death using Suspension Cell Culture of Arabidopsis thaliana.- High-Throughput Screening of Chemical Compound Libraries for Modulators of Salicylic Acid Signaling by In Situ Monitoring of Glucuronidase-Based Reporter Gene Expression.- Identification of Chemical Inducers of the Phosphate-Starvation Signaling Pathway in A. thaliana using Chemical Genetics.- Phenotype-Based Screening of Small Molecules to Modify Plant Cell Walls using BY-2 Cells.- Small Molecule Screening to Increase Agrobacterium-Mediated Transformation Efficiency in Legumes.- High-Throughput In Vitro Screeningfor Inhibitors of Cereal ¿-glucosidase.- Chemical Screening for Strigolactone Receptor Antagonists using Arabidopsis thaliana.- Chemical Control of ABA Receptors to Enable Plant Protection against Water Stress.- Modes of Action Study of Seed Germination Inhibitor Germostatin by Forward Genetics Screening.- Enantiodifferential Approach for the Target Protein Detection of the Jasmonate Glucoside that Controls the Leaf Closure of Samanea saman.- Using the QCM Biosensor-Based T7 Phage Display Combined with Bioinformatics Analysis for Target Identification of Bioactive Small Molecule.- Genomic Identification and Analysis of Specialized Metabolite Biosynthetic Gene Clusters in Plants using PlantiSMASH.- Chemical Genomics Translatability from Unicellular to Multicellular Models.- Chemical Screening for Flagella-Associated Phenotypes in Chlamydomonas reinhardtii.- FnCpf1-Mediated Targeted Mutagenesis in Plants.
Klappentext
This volume discusses easy-to-follow protocols for researchers in the field of plant chemical genomics. The chapters in this book look at technical workflows of high-throughput chemical compound screens in numerous plant tissues, such as seedlings, roots, and pollen. Chapters also focus on single-celled systems such as Chlamydomonas reinhardtii for chemical screens, and techniques used to modify the target of a small molecule. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
rnThorough and cutting-edge, Plant Chemical Genomics: Methods and Protocols is a valuable resource to help researchers work with a broad variety of plants for chemical genomics approaches.
rn
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
