Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale `omics´ methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.
1. The Eukaryotic Pathogen Databases: A Functional Genomic Resource Integrating Data from of Human and Veterinary Parasites
Omar S. Harb and David S. Roos
2. From Sequence Mapping to Genome Assemblies
Thomas D. Otto
3. Sequencing and Annotation of Mitochondrial Genomes from Individual Parasitic Helminths
Aaron R. Jex, D. Timothy Littlewood, and Robin B. Gasser
4. A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-coding Genes in a Genome
Daniel C. Jeffares, Bartlomiej Tomiczek,Victor Sojo, and Mario dos Reis
5. Exploiting Genetic Variation to Discover Genes Involved in Important Disease Phenotypes
Paul Capewell, Anneli Cooper, Caroline Clucas, Willie Weir, Heli Vaikkinen, Liam Morrison, Andy Tait, and Annette MacLeod
6. Identification and Analysis of Ingi-Related Retroposons in the Trypanosomatid Genomes
Frédéric Bringaud, Matthew Rogers, and Elodie Ghedin
7. Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in Leishmania
Barbara Papadopoulou, Michaela Müller-McNicoll, and Prasad K. Padmanabhan
8. Gene Suppression in Schistosomes using RNAi
Akram A. Da´dara and Patrick J. Skelly
9. Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Tranillegalscript Ends
Nikolay G. Kolev, Elisabetta Ullu and Christian Tschudi
10. Techniques to Study Epigenetic Control and the Epigenome in Parasites
Sheila C. Nardelli, Li-Min Ting, and Kami Kim
11. The Genome-wide Identification of Promoter Regions in Toxoplasma gondii
Junya Yamagish and Yutaka Suzuki
12. RNA-seq Approaches for Determining mRNA Abundance in Leishmania
Andrew Haydock, Monica Terrao, Aarthi Sekar, Gowthaman Ramasamy, Loren Baugh, and Peter J. Myler
13. Protein Microarrays for Parasite Antigen Discovery
Patrick Driguez, Denise L Doolan, Douglas M Molina, Alex Loukas, Angela Trieu, Phil L Felgner, and Donald P McManus
14. A Transposon-based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa
Jeziel D. Damasceno, Stephen M. Beverley, and Luiz R. O. Tosi
15. Separation of Basic Proteins from Leishmania using a combination of Free Flow Electrophoresis (FFE) and 2D Electrophoresis (2-DE) under Basic Conditions
Marie-Christine Brotherton, Gina Racine, and Marc Ouellette
16. Proteomic Analysis of Post Translational Modifications using iTRAQ in Leishmania
Dan Zilberstein
17. Large Scale Differential Proteome Analysis in Plasmodium falciparum under Drug Treatment
Judith Helena Prieto, Elisabeth Fisher, Sasa Koncarevic, John Yates, and Katja Becker
18. Use of 13C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in Leishmania Parasites
Eleanor C. Saunders, David P. de Souza, Jennifer M. Chambers, Milica Ng, James Pyke, and Malcolm J. McConville
19. Molecular Genotyping of Trypanosoma cruzi for Lineage Assignment and Population Genetics
Louisa A. Messenger, Matthew Yeo, Michael D. Lewis, Martin S. Llewellyn, and Michael A. Miles
20. Screening Leishmania donovani Complex-specific Genes Required for Visceral Disease
Wen-Wei Zhang and Greg Matlashewski
Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale `omics´ methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.
Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale 'omics' methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.
The Eukaryotic Pathogen Databases: A Functional Genomic Resource Integrating Data from of Human and Veterinary Parasites.- From Sequence Mapping to Genome Assemblies.- Sequencing and Annotation of Mitochondrial Genomes from Individual Parasitic Helminths.- A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-coding Genes in a Genome.- Exploiting Genetic Variation to Discover Genes Involved in Important Disease Phenotypes.- Identification and Analysis of Ingi-Related Retroposons in the Trypanosomatid Genomes.- Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in Leishmania.- Gene Suppression in Schistosomes using RNAi.- Construction of Trypanosoma brucei Illumina RNA-Seq Libraries Enriched for Tranillegalscript Ends.- Techniques to Study Epigenetic Control and the Epigenome in Parasites.- The Genome-wide Identification of Promoter Regions in Toxoplasma gondii.- RNA-seq Approaches for Determining mRNA Abundance in Leishmania.- Protein Microarrays for Parasite Antigen Discovery.- A Transposon-based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa.- Separation of Basic Proteins from Leishmania using a combination of Free Flow Electrophoresis (FFE) and 2D Electrophoresis (2-DE) under Basic Conditions.- Proteomic Analysis of Post Translational Modifications using iTRAQ in Leishmania.- Large Scale Differential Proteome Analysis in Plasmodium falciparum under Drug Treatment.- Use of 13C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in Leishmania Parasites.- Molecular Genotyping of Trypanosoma cruzi for Lineage Assignment and Population Genetics.- Screening Leishmania donovani Complex-specific Genes Required for Visceral Disease.
Inhaltsverzeichnis
The Eukaryotic Pathogen Databases: A Functional Genomic Resource Integrating Data from of Human and Veterinary Parasites.- From Sequence Mapping to Genome Assemblies.- Sequencing and Annotation of Mitochondrial Genomes from Individual Parasitic Helminths.- A Beginners Guide to Estimating the Non-synonymous to Synonymous Rate Ratio of all Protein-coding Genes in a Genome.- Exploiting Genetic Variation to Discover Genes Involved in Important Disease Phenotypes.- Identification and Analysis of Ingi-Related Retroposons in the Trypanosomatid Genomes.- Approaches for Studying mRNA Decay Mediated by SIDER2 Retroposons in
Leishmania
.- Gene Suppression in Schistosomes using RNAi.- Construction of
Trypanosoma brucei
Illumina RNA-Seq Libraries Enriched for Tranillegalscript Ends.- Techniques to Study Epigenetic Control and the Epigenome in Parasites.- The Genome-wide Identification of Promoter Regions in
Toxoplasma gondii
.- RNA-seq Approaches for Determining mRNA Abundance in
Leishmania.-
Protein Microarrays for Parasite Antigen Discovery.- A Transposon-based Tool for Transformation and Mutagenesis in Trypanosomatid Protozoa.- Separation of Basic Proteins from
Leishmania
using a combination of Free Flow Electrophoresis (FFE) and 2D Electrophoresis (2-DE) under Basic Conditions.- Proteomic Analysis of Post Translational Modifications using iTRAQ in
Leishmania
.- Large Scale Differential Proteome Analysis in
Plasmodium falciparum
under Drug Treatment.- Use of
13
C Stable Isotope Labelling for Pathway and Metabolic Flux Analysis in
Leishmania
Parasites.- Molecular Genotyping of
Trypanosoma cruzi
for Lineage Assignment and Population Genetics.- Screening
Leishmania donovani
Complex-specific Genes Required for Visceral Disease.
Klappentext
Parasite Genomics Protocols, Second Edition expands upon the previous edition with current, detailed methodologies that have been adapted to research parasites in the post genomic era. With new chapters on parasite studies, helminth pathogens, and protocols for undertaking other large-scale ¿omics¿ methodologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nAuthoritative and practical, Parasite Genomics Protocols, Second Edition offers a wide-ranging collection of diverse methods and protocols for further study into these unique organisms.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
