Elizabeth Rakcozy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the down-regulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of most powerful tools with which to ask--and answer--all the important questions emerging from the dramatically advancing work in vision research today.
Basic Molecular Biology Techniques, Chooi-May Lai. Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes, Debora B. Farber, Leonid E. Lerner, and Andrea S. Viczian. In Situ Hybridization, Xian-Jie Yang. Immunocytochemical Studies of the Retina, Ann H. Milam. Antisense DNA Technology, Piroska Elizabeth Rakoczy. Inhibition of Gene Expression by Ribozymes, William W. Hauswirth, Lynn C. Shaw, Patrick O. Whalen, Jason J. Fritz, D. Alan White, and Alfred S. Lewin. Intraocular Delivery of Recombinant Virus, Fong-Qi Liang, Vibha Anand, Albert M. Maguire, and Jean Bennett. Preparation of Recombinant Adenoviruses, Karl G. Csaky. Generation of Recombinant Adeno-Associated Virus, Fabienne Rolling. Preparation of Recombinant Retroviruses, Xian-Jie Yang. Generation of Transgenic Mice for Studies of Ocular Development and Disease, Abha R. Gupta, Nadine S. Dejneka, Albert M. Maguire, and Jean Bennett. Generation of Knockout Animal Models, T. Michael Redmond. Mutation Analysis of Ocular Genes, Michael Danciger. Ophthalmologists' Perspective, Susan M. Downes, N. H. Victor Chong, and Alan C. Bird. Index.
Much fascinating and valuable research has been devoted to the molecular biology of the eye, its various structures, and its innervation this past decade. In Vision Research Protocols, Elizabeth Rakoczy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the downregulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. The book details the three most popular viral delivery vehicles--recombinant adeno, adeno-associated, and retroviruses-as well as the major delivery methods for the most widely used animal models.
Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of the most powerful tools with which to ask--and answer--all the important questions emerging from the dramatically advancing work on vision research today.
"...a highly detailed book on experimental techniques in vision research...highly recommended book for everyone with an interest in molecular biology and eye research." -Acta Ophthalmologica Scandinavica
Basic Molecular Biology Techniques.- Transient Transfection of Human Retinoblastoma Cells.- In Situ Hybridization.- Immunocytochemical Studies of the Retina.- Antisense DNA Technology.- Inhibition of Gene Expression by Ribozymes.- Intraocular Delivery of Recombinant Virus.- Preparation of Recombinant Adenoviruses.- Generation of Recombinant Adeno-Associated Virus.- Preparation of Recombinant Retroviruses.- Generation of Transgenic Mice for Studies of Ocular Development and Disease.- Generation of Knockout Animal Models.- Mutation Analysis of Ocular Genes.- Ophthalmologists' Perspective.
Inhaltsverzeichnis
Basic Molecular Biology Techniques, Chooi-May Lai. Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes, Debora B. Farber, Leonid E. Lerner, and Andrea S. Viczian. In Situ Hybridization, Xian-Jie Yang. Immunocytochemical Studies of the Retina, Ann H. Milam. Antisense DNA Technology, Piroska Elizabeth Rakoczy. Inhibition of Gene Expression by Ribozymes, William W. Hauswirth, Lynn C. Shaw, Patrick O. Whalen, Jason J. Fritz, D. Alan White, and Alfred S. Lewin. Intraocular Delivery of Recombinant Virus, Fong-Qi Liang, Vibha Anand, Albert M. Maguire, and Jean Bennett. Preparation of Recombinant Adenoviruses, Karl G. Csaky. Generation of Recombinant Adeno-Associated Virus, Fabienne Rolling. Preparation of Recombinant Retroviruses, Xian-Jie Yang. Generation of Transgenic Mice for Studies of Ocular Development and Disease, Abha R. Gupta, Nadine S. Dejneka, Albert M. Maguire, and Jean Bennett. Generation of Knockout Animal Models, T. Michael Redmond. Mutation Analysis of Ocular Genes, Michael Danciger. Ophthalmologists' Perspective, Susan M. Downes, N. H. Victor Chong, and Alan C. Bird. Index.
Klappentext
Much fascinating and valuable research has been devoted to the molecular biology of the eye, its various structures, and its innervation this past decade. In Vision Research Protocols, Elizabeth Rakoczy and a team of leading clinical and experimental scientists describe in step-by-step detail the key techniques essential to effective molecular biological research in ophthalmology and optometry. These readily reproducible methods are adapted to the special requirements of vision research, with coverage that ranges from the most basic to the most sophisticated technologies. Included are methods for the downregulation of gene expression, new gene therapy techniques, and for the development of transgenic and knockout animal models for testing novel therapies. The book details the three most popular viral delivery vehicles--recombinant adeno, adeno-associated, and retroviruses-as well as the major delivery methods for the most widely used animal models.
Eminently accessible and clinically relevant, Vision Research Protocols provides experimental and biomedical investigators in ophthalmology and optometry with a rich panoply of the most powerful tools with which to ask--and answer--all the important questions emerging from the dramatically advancing work on vision research today.
Includes supplementary material: sn.pub/extras
