Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes, Fumio Nakamura, Yoshio Goshima, Stephem M. Struttmatter, and Susumu Kawamoto. Expression of NMDA Receptor Channel Subunit Proteins using Baculovirus and Herpesvirus Vectors, Susumu Kawamoto, Satoshi Hattori, Shigeo Uchino Masayoshi Mishina, and Kenji Okuda. Transient Expression of Functional NMDA Receptors in Mammalian Cells, Paul L. Chazot, Miroslav Cik, and F. Anne Stephenson. Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells, Mark A. Varney, Fen-Fen Lin, Christine Jachec, Sara P. Rao, Stephen D. Hess, Edwin C. Johnson, and Gönül Veliçelibi. Expression of Extracellular N-terminal Domain of NMDA Receptor in Mammalian Cells, Erik A. Whitehorn, William J. Dower, and Min Li. Immunocytochemistry of NMDA Receptors, Ronald S. Petralia and Robert J. Wenthold. The Use of Ligand Binding in Assays of NMDA Receptor Function, Ian J. Reynolds and Terre A. Sharma. Calmodulin Modification of NMDA Receptors, Su Zhang and Richard L. Huganir. Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors, Robert J. Wenthold, Jaroslav Blahos II, Kyung-Hye Huh, and Ronald S. Petralia. Redox Sensitivity of NMDA Receptors, Stuart Lipton. In Vitro Selection of Peptides Acting on NMDA Receptors, Steven E. Cwirla, William Dower, and Min Li. NMDA Receptor Pharmacololgy and Analysis of Patch-Clamp Receptors, Jerry M. Wright and Robert W. Peoples. Determination of Membrane Topology of Glutamate Receptors, Hendrika M.A. VanDongen and Antonius M.J. VanDongen. Engineering the NMDA Receptor Channel Lining, Antonio V. Ferrer-Montiel and Mauricio Montal. High-Throughput Functional Detection of NMDA Receptor Activity, Martin Donal Dunningham and Sara Mary Cunningham. Index.
In NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors.
NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
"...In this volume of Methods in Molecular Biology great care has been taken to present the most modern approaches to solving the mysteries of the NMDA receptor...Each chapter includes detailed protocols and highly useful 'notes' that explain possible problems, sources of technical artifacts and how to overcome them....this book is informative and practical and would serve any channel or receptor researcher well." -Molecular Biotechnology
Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes.- Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors.- Transient Expression of Functional NMDA Receptors in Mammalian Cells.- Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells.- Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells.- Immunocytochemistry of NMDA Receptors.- The Use of Ligand Binding in Assays of NMDA Receptor Function.- Calmodulin Modification of NMDA Receptors.- Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors.- Redox Sensitivity of NMDA Receptors.- In Vitro Selection of Peptides Acting on NMDA Receptors.- NMDA Receptor Pharmacology and Analysis of Patch-Clamp Recordings.- Determination of Membrane Topology of Glutamate Receptors.- Engineering the NMDA Receptor Channel Lining.- High-Throughput Functional Detection of NMDA Receptor Activity.
Inhaltsverzeichnis
Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes, Fumio Nakamura, Yoshio Goshima, Stephem M. Struttmatter, and Susumu Kawamoto. Expression of NMDA Receptor Channel Subunit Proteins using Baculovirus and Herpesvirus Vectors, Susumu Kawamoto, Satoshi Hattori, Shigeo Uchino Masayoshi Mishina, and Kenji Okuda. Transient Expression of Functional NMDA Receptors in Mammalian Cells, Paul L. Chazot, Miroslav Cik, and F. Anne Stephenson. Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells, Mark A. Varney, Fen-Fen Lin, Christine Jachec, Sara P. Rao, Stephen D. Hess, Edwin C. Johnson, and Gönül Veliçelibi. Expression of Extracellular N-terminal Domain of NMDA Receptor in Mammalian Cells, Erik A. Whitehorn, William J. Dower, and Min Li. Immunocytochemistry of NMDA Receptors, Ronald S. Petralia and Robert J. Wenthold. The Use of Ligand Binding in Assays of NMDA Receptor Function, Ian J. Reynolds and Terre A. Sharma. Calmodulin Modification of NMDA Receptors, Su Zhang and Richard L. Huganir. Detergent Solubilization and Immunoprecipitation of Native NMDA Receptors, Robert J. Wenthold, Jaroslav Blahos II, Kyung-Hye Huh, and Ronald S. Petralia. Redox Sensitivity of NMDA Receptors, Stuart Lipton. In Vitro Selection of Peptides Acting on NMDA Receptors, Steven E. Cwirla, William Dower, and Min Li. NMDA Receptor Pharmacololgy and Analysis of Patch-Clamp Receptors, Jerry M. Wright and Robert W. Peoples. Determination of Membrane Topology of Glutamate Receptors, Hendrika M.A. VanDongen and Antonius M.J. VanDongen. Engineering the NMDA Receptor Channel Lining, Antonio V. Ferrer-Montiel and Mauricio Montal. High-Throughput Functional Detection of NMDA Receptor Activity, Martin Donal Dunningham and Sara Mary Cunningham. Index.
Klappentext
Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
Includes supplementary material: sn.pub/extras
