Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen,
Chlamydia trachomatis. These include techniques to identify
Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate
Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and deillegalscriptions of genotyping technologies, as well as dual RNA-Seq tranillegalscriptional profiling. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.
1. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA)
Kevin E. Eboigbodin and Mark Hoser
2. Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue
Naraporn Somboonna and Ilada Choopara
3. Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
Kevin E. Eboigbodin
4. Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay
Kevin E. Eboigbodin
5. Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth
Tomislav Meštrovic, Dezso P. Virok, Suncanica Ljubin-Sternak, Tímea Raffai, Katalin Burián, and Jasmina Vraneš
6. Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples
Amanda Claire Brown and Mette T. Christiansen
7. Multi Locus Sequence Typing (MLST) of Chlamydiales
Martina Jelocnik, Adam Polkinghorne, and Yvonne Pannekoek
8. Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method
Deborah C. Holt, Patiyan Andersson, Cameron Buckley, David M. Whiley, and Phillip M. Giffard
9. Dual RNA-Seq of Chlamydia and Host Cells
James W. Marsh, Regan Hayward, Amol Shetty, Anup Mahurkar, Michael S. Humphrys, and Garry S.A. Myers
10. Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection
Oleg V. Podgorny, Nadezhda F. Polina, and Vassili N. Lazarev
11. Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions
Katerina Wolf, Mostafa Rahnama, and Kenneth A. Fields
12. Mutagenesis of Chlamydia trachomatis Using TargeTron
Mary M. Weber and Robert Faris
13. Forward and Reverse Genetic Analysis of Chlamydia
Mateusz Kedzior and Robert J. Bastidas
14. A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions
Rachel Ende and Isabelle Derré
15. Rodent Infections for Chlamydia spp.
Charles W. Armitage, Alison J. Carey, and Kenneth W. Beagley
16. Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy
Steven Liang and James B. Mahony
17. Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane
Macy G. Olson, Lisa M. Jorgenson, Ray E. Widner, and Elizabeth A. Rucks
18. High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis
Amanda Claire Brown and Nicole L. Kushner
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen,
Chlamydia trachomatis. These include techniques to identify
Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate
Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and deillegalscriptions of genotyping technologies, as well as dual RNA-Seq tranillegalscriptional profiling. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.
"Students, postgraduates, scientists and researchers, public health researchers, and microbiologists will find this book of immense interest. ... This is an important book with current information on cutting-edge methods and protocols for Chlamydia trachomatis. Given the rapid advances in this field, this book will appeal to its intended audience.” (Omer Iqbal, Doody´s Book Reviews, January 27, 2020)
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen,
Chlamydia trachomatis. These include techniques to identify
Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate
Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and deillegalscriptions of genotyping technologies, as well as dual RNA-Seq tranillegalscriptional profiling. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative, practical, and relevant,
Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.
Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA).- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue.- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae.- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay.- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth.- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples.- Multi Locus Sequence Typing (MLST) of Chlamydiales.- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method.- Dual RNA-Seq of Chlamydia and Host Cells.- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection.- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions.- Mutagenesis of Chlamydia trachomatis Using TargeTron.- Forward and Reverse Genetic Analysis of Chlamydia.- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions.- Rodent Infections for Chlamydia spp..- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy.- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane.- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis.
"Students, postgraduates, scientists and researchers, public health researchers, and microbiologists will find this book of immense interest. ... This is an important book with current information on cutting-edge methods and protocols for Chlamydia trachomatis. Given the rapid advances in this field, this book will appeal to its intended audience." (Omer Iqbal, Doody's Book Reviews, January 27, 2020)
Inhaltsverzeichnis
Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Multiplex Strand Invasion-Based Amplification (mSIBA).- Point of Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue.- Application of Loop-Mediated Isothermal Amplification Assay for the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae.- Simultaneous Detection of Chlamydia trachomatis and Neisseria gonorrhoeae Using Real-Time Multiplex qPCR Assay.- Antimicrobial Resistance Screening in Chlamydia trachomatis by Optimized McCoy Cell Culture System and Direct qPCR-Based Monitoring of Chlamydial Growth.- Whole Genome Sequencing of Chlamydia trachomatis Directly from Human Samples.- Multi Locus Sequence Typing (MLST) of Chlamydiales.- Identification and Discrimination of Chlamydia trachomatis Ocular and Urogenital Strains and Major Phylogenetic Lineages by CtGEM Typing, a Double Locus Genotyping Method.- Dual RNA-Seq of Chlamydia and Host Cells.- Isolation and Propagation of Single Inclusion-Derived Chlamydia Using Laser Microdissection.- Genetic Manipulation of Chlamydia trachomatis: Chromosomal Deletions.- Mutagenesis of Chlamydia trachomatis Using TargeTron.- Forward and Reverse Genetic Analysis of Chlamydia.- A Co-Infection Model to Evaluate Chlamydia Inc Protein Interactions.- Rodent Infections for Chlamydia spp..- Enumeration of Viable Chlamydia from Infected Animals Using Immunofluorescent Microscopy.- Proximity Labeling of the Chlamydia trachomatis Inclusion Membrane.- High-Throughput Screening for Novel Inhibitors of Intracellular Pathogens, including Chlamydia trachomatis.
Klappentext
This book explores cutting-edge methods to work with the notoriously difficult, but highly prevalent, obligate intracellular pathogen, Chlamydia trachomatis. These include techniques to identify Chlamydia trachomatis in patient samples, ranging from simple point-of-care tests to whole genome sequencing; methods for propagation of strains in both cell culture and animal models; techniques to manipulate Chlamydia trachomatis in molecular genetic methodologies; a high-throughput screening method for testing new potential drugs against intracellular bacteria; a screen for antibiotic resistance; methods for labeling and enumeration; and deillegalscriptions of genotyping technologies, as well as dual RNA-Seq tranillegalscriptional profiling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Authoritative, practical, and relevant, Chlamydia trachomatis: Methods and Protocols serves as an ideal reference for scientists searching for a better understanding of the pathogen, allowing for the development of improved treatment regimens and the discovery of new drugs.
Includes cutting-edge techniques
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
