Includes cutting-edge methods and protocols for the study of epithelial cell culture
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Mario Baratta is Full professor in Veterinary Physiology at the University of Turin (IT). He got the master degree in Veterinary Medicine at the University of Parma (IT) and the PhD in Neuroendocrinology of Animal Farm at the University of Bologna (IT). Today he is the Coordinator of the Graduate School in Veterinary Science for Animal Health and Food Safety, University of Turin. His main interests are the biology of adult stem cells in different tissues and he has developed specific knowledge in functional assessment systems of epithelial cells and cell differentiation in mammary gland and in muscle in animal science.
This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
1. Murine and human cancer mammary cell lines: functional tests
2. Bovine mammary organoids: a model to study epithelial mammary cells
3. Equine primary granulosa cell culture: analyses of tranillegalscriptome
4. Amniotic epithelial cells culture
5. Models of the small and large Intestinal epithelium: epithelial cell coculture
6. Differentiation of lung alveolar epithelial cells In Vitro
7. GnRH receptor analyses in pituitary ?T3-1 cell line
8. Normal human thyrocytes in culture
9. Pig blastocyst culture: evaluation of maturation and steroid production
10. Primary pituitary cell culture in goldfish: hormonal analyses
11. Gastric Organoids: An Emerging Model System
12. Olfactory epithelium culture
13. Characterization of a coculture system of equine endometrial epithelial and stromal cells
14. Bovine endometrial epithelial cells analysis in protein expression
15. 3D Culture of pancreatic cells on decellularized Pancreatic bio-Scaffold
16. (3D) in vitro model replicating the intestinal functioning to dissect the stromal-epithelial interactions and evaluate the permeation of a model drug
17. Gastrospheres of human gastric mucosa cells: an in vitro model of stromal and epithelial stem cell niche reconstruction
18. Bovine lactotroph culture for the study of prolactin synthesis function
19. Granulosa cell coculture system to cultivate pig embryos
20. 3D primary epithelial cells culture system in mammary cell line
21. Mammospheres from murine mammary stem cell-enriched system
22. Three-dimensional cell culture model utilization in cancer stem cell research
23. Follicle-like model by granulosa cell encapsulation
24. Rat Pancreatic Islets in a Dynamic Culture
This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written forthe highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
Normal Human Thyrocytes in Culture.- Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia.- Reassembly of Functional Human Salivary Stem/Progenitor Cells in 3D Culture.- Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro.- Differentiation of gastrointestinal cell lines by culture in semi-wet interface.- Three-dimensional cell culture model utilization in renal carcinoma cancer stem cell research.- Amniotic epithelial cell culture. Corresponding author.- Bovine granulosa cell culture.- Bioencapsulation of oocytes and granulosa cells.- Ovine granulosa cells isolation and culture to improve oocyte quality.- 3D model replicating the intestinal function to evaluate drug permeability.- Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture.- Long-term culture of intestinal organoids.- Bovine mammary organoids: a model to study epithelial mammary cells.- Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from organoids.- Mouse-Derived Gastric Organoid and Immune Cell Co-Culture for the Study of the Tumor Microenvironment.- Murine and human mammary cancer cell lines: functional tests.- In vitro porcine colon culture.- Primary cultures of olfactory neurons from the avian olfactory epithelium.
Mario Baratta is Full professor in Veterinary Physiology at the University of Turin (IT). He got the master degree in Veterinary Medicine at the University of Parma (IT) and the PhD in Neuroendocrinology of Animal Farm at the University of Bologna (IT). Today he is the Coordinator of the Graduate School in Veterinary Science for Animal Health and Food Safety, University of Turin. His main interests are the biology of adult stem cells in different tissues and he has developed specific knowledge in functional assessment systems of epithelial cells and cell differentiation in mammary gland and in muscle in animal science.
Über den Autor
rn Mario Baratta is Full professor in Veterinary Physiology at the University of Turin (IT). He got the master degree in Veterinary Medicine at the University of Parma (IT) and the PhD in Neuroendocrinology of Animal Farm at the University of Bologna (IT). Today he is the Coordinator of the Graduate School in Veterinary Science for Animal Health and Food Safety, University of Turin. His main interests are the biology of adult stem cells in different tissues and he has developed specific knowledge in functional assessment systems of epithelial cells and cell differentiation in mammary gland and in muscle in animal science.
rn
Inhaltsverzeichnis
Normal Human Thyrocytes in Culture.- Isolation and Culture of Juvenile Pig Thyroid Follicular Epithelia.- Reassembly of Functional Human Salivary Stem/Progenitor Cells in 3D Culture.- Culture and Differentiation of Lung Bronchiolar Epithelial Cells In Vitro.- Differentiation of gastrointestinal cell lines by culture in semi-wet interface.- Three-dimensional cell culture model utilization in renal carcinoma cancer stem cell research.- Amniotic epithelial cell culture. Corresponding author.- Bovine granulosa cell culture.- Bioencapsulation of oocytes and granulosa cells.- Ovine granulosa cells isolation and culture to improve oocyte quality.- 3D model replicating the intestinal function to evaluate drug permeability.- Isolation of Human Gastric Epithelial Cells from Gastric Surgical Tissue and Gastric Biopsies for Primary Culture.- Long-term culture of intestinal organoids.- Bovine mammary organoids: a model to study epithelial mammary cells.- Establishment of Human- and Mouse-Derived Gastric Primary Epithelial Cell Monolayers from organoids.- Mouse-Derived Gastric Organoid and Immune Cell Co-Culture for the Study of the Tumor Microenvironment.- Murine and human mammary cancer cell lines: functional tests.- In vitro porcine colon culture.- Primary cultures of olfactory neurons from the avian olfactory epithelium.
Klappentext
This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written forthe highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
Includes cutting-edge methods and protocols for the study of epithelial cell culture
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
