Includes cutting-edge techniques for the study of epigenome editing
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Includes cutting-edge techniques for the study of epigenome editing
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts
Part I: General Reviews
1. Editing the Epigenome: Overview, Open Questions, and Directions of Future Development
Marianne G. Rots and Albert Jeltsch
2. Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing
Charlene Babra Waryah, Colette Moses, Mahira Arooj, and Pilar Blancafort
3. Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities
Dilara Sen and Albert J. Keung
4. Generation of TALE-Based Designer Epigenome Modifiers
Sandra Nitsch and Claudio Mussolino
Part II: Topical Reviews
5. Neuroepigenetic Editing
Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller
6. Allele-Specific Epigenome Editing
Pavel Bashtrykov and Albert Jeltsch
7. Key to Delivery: The (Epi-)Genome Editing Vector Toolbox
Sabrina Just and Hildegard Büning
8. CRISPR/dCas9 Switch Systems for Temporal Tranillegalscriptional Control
Rutger A.F. Gjaltema and Edda G. Schulz
Part III: Delivery Protocols
9. Delivery of Designer Epigenome Modifiers into Primary Human T Cells
Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, and Claudio Mussolino
10. Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques
Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, and Elizabeth A. Heller
11. Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration
Carolin Kroll and Philipp Rathert
12. Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain
Benjamin Pyles, Barbara J. Bailus, Henriette O´Geen, and David J. Segal
13. Non-Viral Methodology for Efficient Co-Transfection
Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, and K. Swaminathan Iyer
Part IV: Histone Editing Readout Protocols
14. Chromatin Immunoprecipitation in Human and Yeast Cells
Jessica B. Lee and Albert J. Keung
15. Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis
Darren K. Patten, Giacomo Corleone, and Luca Magnani
Part V: DNA Methylation Editing Readout Protocols
16. Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis
Dulce B. Vargas-Landin, Jahnvi Pflüger, and Ryan Lister
17. Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data
Tim Stuart, Sam Buckberry, and Ryan Lister
18. Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution
Magali Kernaleguen, Christian Daviaud, Yimin Shen, Eric Bonnet, Victor Renault, Jean-François Deleuze, Florence Mauger, and Jörg Tost
19. Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing
Elsa Leitão, Jasmin Beygo, Michael Zeschnigk, Ludger Klein-Hitpass, Marcel Bargull, Sven Rahmann, and Bernhard Horsthemke
20. DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS)
Pavel Bashtrykov and Albert Jeltsch
Part VI: Gene Expression Readout Protocols
21. Cell-to-Cell Tranillegalscription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching
William Beckman, Ilona M. Vuist, Hermannus Kempe, and Pernette J. Verschure
22. Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing
Désirée Goubert, Mihály Koncz, Antal Kiss, and Marianne G. Rots
Part VII: Special Application Protocols
23. Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions
Sumiyo Morita, Takuro Horii, and Izuho Hatada
24. Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells
Dan Gao and Fu-Sen Liang
25. Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing
Tyler S. Klann, Gregory E. Crawford, Timothy E. Reddy, and Charles A. Gersbach
This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.
This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.
Editing the Epigenome: Overview, Open Questions, and Directions of Future Development.- Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing.- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities.- Generation of TALE-Based Designer Epigenome Modifiers.- Neuroepigenetic Editing.- Allele-Specific Epigenome Editing.- Key to Delivery: The (Epi-)Genome Editing Vector Toolbox.- CRISPR/dCas9 Switch Systems for Temporal Tranillegalscriptional Control.- Delivery of Designer Epigenome Modifiers into Primary Human T Cells.- Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques.- Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration.- Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain.- Non-Viral Methodology for Efficient Co-Transfection.- Chromatin Immunoprecipitation in Human and Yeast Cells.- Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis.- Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis.- Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data.- Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution.- Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing.- DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS).- Cell-to-Cell Tranillegalscription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching.- Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing.- Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions.- Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells.- Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing.
Inhaltsverzeichnis
Editing the Epigenome: Overview, Open Questions, and Directions of Future Development.- Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing.- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities.- Generation of TALE-Based Designer Epigenome Modifiers.- Neuroepigenetic Editing.- Allele-Specific Epigenome Editing.- Key to Delivery: The (Epi-)Genome Editing Vector Toolbox.- CRISPR/dCas9 Switch Systems for Temporal Tranillegalscriptional Control.- Delivery of Designer Epigenome Modifiers into Primary Human T Cells.- Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques.- Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration.- Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain.- Non-Viral Methodology for Efficient Co-Transfection.- Chromatin Immunoprecipitation in Human and Yeast Cells.- Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis.- Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis.- Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data.- Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution.- Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing.- DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS).- Cell-to-Cell Tranillegalscription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching.- Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing.- Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions.- Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells.- Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing.
Klappentext
This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
n
Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.
Includes cutting-edge techniques for the study of epigenome editing
n
Provides step-by-step detail essential for reproducible results
n
Contains key implementation advice from the experts
n
