Includes cutting-edge methods and protocols for meiosis research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols for meiosis research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and practical, Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.
1. Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae
Yona Kassir and David T. Stuart
2. Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe
Randy W. Hyppa, Kyle R. Fowler, and Gerald R. Smith
3. Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast
Isabel Lam, Neeman Mohibullah, and Scott Keeney
4. Ribosome Profiling for the Analysis of Translation During Yeast Meiosis
Caia Duncan and Juan Mata
5. Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation
David T. Stuart
6. Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora
Frederick J. Bowring, P. Jane Yeadon, and David E.A. Catcheside
7. High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae
Yona Kassir
8. Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast
Amy J. MacQueen and Beth Rockmill
9. In Vivo Imaging of Budding Yeast Meiosis
Michael Pollard and Jennifer C. Fung
10. Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I
Lorinda K. Anderson
11. Flow Cytometry for the Isolation and Characterization of Rodent Meiocytes
Adriana Geisinger and Rosana Rodríguez-Casuriaga
12. Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH
Harry Scherthan
13. Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy
Simon I.R. Lane, Stephen Crouch, and Keith T. Jones
14. Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes
Stacie E. Hughes and R. Scott Hawley
15. Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes
Matthew S. Savoian
16. Drosophila Male Meiosis
Silvia Bonaccorsi and Maurizio Gatti
17. Analysis of Chromatin Dynamics During Drosophila Spermatogenesis
Tim Hundertmark, Ina Theofel, Zeynep Eren-Ghiani, David Miller, and Christina Rathke
18. Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model< Martin A. White, Shunxin Wang, Liangran Zhang, and Nancy Kleckner
19. A Computational Approach to Study Gene Expression Networks
Amir Rubinstein and Yona Kassir
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailed and practical,
Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailedand practical,
Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.
Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe.- Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.- Ribosome Profiling for the Analysis of Translation During Yeast Meiosis.- Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation.- Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora.- High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.- In Vivo Imaging of Budding Yeast Meiosis.- Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I.- Flow Cytometry for theIsolation and Characterization of Rodent Meiocytes.- Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH.- Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy.- Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes.- Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes.- Drosophila Male Meiosis.- Analysis of Chromatin Dynamics During Drosophila Spermatogenesis.- Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model.- A Computational Approach to Study Gene Expression Networks.
Inhaltsverzeichnis
Genetic Approaches to Study Meiosis and Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Quantitative Genome-Wide Measurements of Meiotic DNA Double-Strand Breaks and Protein-Binding in S. pombe.- Sequencing Spo11 Oligonucleotides for Mapping Meiotic DNA Double-Strand Breaks in Yeast.- Ribosome Profiling for the Analysis of Translation During Yeast Meiosis.- Selection of G1 Phase Yeast Cells for Synchronous Meiosis and Sporulation.- Fluorescent Protein as a Tool for Investigating Meiotic Recombination in Neurospora.- High Throughput Screening to Identify Regulators of Meiosis-Specific Gene Expression in Saccharomyces cerevisiae.- Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast.- In Vivo Imaging of Budding Yeast Meiosis.- Sequential Immunofluorescent Light Microscopy and Electron Microscopy of Recombination Nodules During Meiotic Prophase I.- Flow Cytometry for theIsolation and Characterization of Rodent Meiocytes.- Imaging of Chromosome Dynamics in Mouse Testis Tissue by Immuno-FISH.- Imaging Chromosome Separation in Mouse Oocytes by Responsive 3D Confocal Timelapse Microscopy.- Live Imaging of Meiosis I in Late Stage Drosophila melanogaster Oocytes.- Microscopy Methods for Analysis of Spindle Dynamics in Meiotic Drosophila Spermatocytes.- Drosophila Male Meiosis.- Analysis of Chromatin Dynamics During Drosophila Spermatogenesis.- Modeling of Meiotic Crossover Interference as Inspired by the Beam-Film Model.- A Computational Approach to Study Gene Expression Networks.
Klappentext
This updated book includes meiosis methods ranging from classical genetic approaches with budding yeast to high resolution microscopy and computational methods for the analysis of recombination and modeling gene expression networks. Cutting-edge procedures for the analysis of double strand breaks at single nucleotide resolution, analysis of translation by ribosome profiling, the use of fluorescent markers to analyze recombination, and strategies for the use of conditional expression to study chromatin protein dynamics are detailed. Advanced cytology methods for live and fixed cell microscopy and image analysis for yeast, drosophila, and mouse are also included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Detailedand practical, Meiosis, Second Edition will prove to be invaluable to biologists, geneticists, biochemists, and anyone investigating meiosis, recombination, and cellular differentiation.
Includes cutting-edge methods and protocols for meiosis research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
