Presents current up-to-date methodologies
Features tips on trouble shooting and avoiding known pitfalls
Contains key notes and implementation advice from the experts
The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.
1. Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins
Andrey S. Tsvetkov, D. Michael Ando & Steven Finkbeiner
2. Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces
Kathleen A. Burke and Justin Legleiter
3. Morphometric Analysis of Huntington´s Disease Neurodegeneration in Drosophila
Wan Song, Marianne R. Smith, Adeela Syed, Tamas Lukacsovich, Brett A. Barbaro, Judith Purcell, Doug J. Bornemann, John Burke and J. Lawrence Marsh
4. Size Analysis of Polyglutamine Protein Aggregates using Fluorescence Detection in an Analytical UltracentrifugeSaskia Polling, Danny M. Hatters, and Yee-Foong Mok
5. A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins
Amy L. Robertson and Stephen P. Bottomley
6. Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation
Yasmin M. Ramdzan, Rebecca Wood, Danny M. Hatters
7. Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders
Emma L. Burrows and Anthony J. Hannan
8. PCR Amplification and Sequence Analysis of GC-rich Sequences: Aristaless-Related Homeobox Example
Tan M.H., Gécz J., Shoubridge C.
9. Challenges of "sticky” Co-Immunoprecipitation; Polyalanine Tract Protein-Protein Interactions
Mattiske T.R., Tan M.H., Gécz J. and Shoubridge C.
10. Molecular Pathology of Polyalanine Expansion Disorders-new
Perspectives from Mouse Models
James N Hughes and Paul Q Thomas
11. Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation
Martin L. Duennwald
12. Immunobased Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples
Gregor P. Lotz and Andreas Weiss
13. Modeling and Analysis of Repeat RNA Toxicity in Drosophila
Samaraweera, S.E. , O´Keefe, L.V. , van Eyk, C.L. , Lawlor, K.T., Humphreys D.T., Suter, C.M. and Richards, R.I.
14. Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis
Mats Holmberg, Ellen A.A. Nollen
15. Kinetic Analysis of Aggregation Data
Regina M. Murphy
16. A Bioinformatics Method for Identifying Q/N-rich Prion-like Domains in Proteins
Eric D. Ross, Kyle S. MacLea, Charles Anderson, and Asa Ben-Hur
17. Detecting Soluble PolyQ Oligomers and Investigating their Impact on Living Cells using Split-GFP
Patrick Lajoie and Erik Lee Snapp
18. Cell Biological Approaches To Investigate Polyglutamine-Expanded AR Metabolism
Lori J. Cooper and Diane E. Merry
This book details methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. It features step-by-step, readily reproducible laboratory protocols as well as lists of the necessary materials and reagents.
The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.
Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins.- Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces.- Morphometric Analysis of Huntington's Disease Neurodegeneration in Drosophila.- Size Analysis of Polyglutamine Protein Aggregates using Fluorescence Detection in an Analytical Ultracentrifuge.- A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins.- Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation.- Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders.- PCR Amplification and Sequence Analysis of GC-rich Sequences: Aristaless-Related Homeobox Example.- Challenges of "sticky" Co-Immunoprecipitation; Polyalanine Tract Protein-Protein Interactions.- Molecular Pathology of Polyalanine Expansion Disorders-new Perspectives from Mouse Models.- Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation.- Immunobased Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples.- Modeling and Analysis of Repeat RNA Toxicity in Drosophila.- Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis.- Kinetic Analysis of Aggregation Data.- A Bioinformatics Method for Identifying Q/N-rich Prion-like Domains in Proteins.- Detecting Soluble PolyQ Oligomers and Investigating their Impact on Living Cells using Split-GFP.- Cell Biological Approaches To Investigate Polyglutamine-Expanded AR Metabolism.
Inhaltsverzeichnis
1. Longitudinal Imaging and Analysis of Neurons Expressing Polyglutamine-Expanded Proteins
Andrey S. Tsvetkov, D. Michael Ando & Steven Finkbeiner
2. Atomic Force Microscopy Assays for Evaluating Polyglutamine Aggregation in Solution and on Surfaces
Kathleen A. Burke and Justin Legleiter
3. Morphometric Analysis of Huntington's Disease Neurodegeneration in Drosophila
Wan Song, Marianne R. Smith, Adeela Syed, Tamas Lukacsovich, Brett A. Barbaro, Judith Purcell, Doug J. Bornemann, John Burke and J. Lawrence Marsh
4. Size Analysis of Polyglutamine Protein Aggregates using Fluorescence Detection in an Analytical UltracentrifugeSaskia Polling, Danny M. Hatters, and Yee-Foong Mok
5. A Method for the Incremental Expansion of Polyglutamine Repeats in Recombinant Proteins
Amy L. Robertson and Stephen P. Bottomley
6. Pulse Shape Analysis (PulSA) to Track Protein Translocalization in Cells by Flow Cytometry: Applications for Polyglutamine Aggregation
Yasmin M. Ramdzan, Rebecca Wood, Danny M. Hatters
7. Characterizing Social Behavior in Genetically Targeted Mouse Models of Brain Disorders
Emma L. Burrows and Anthony J. Hannan
8. PCR Amplification and Sequence Analysis of GC-rich Sequences: Aristaless-Related Homeobox Example
Tan M.H., Gécz J., Shoubridge C.
9. Challenges of "sticky" Co-Immunoprecipitation; Polyalanine Tract Protein-Protein Interactions
Mattiske T.R., Tan M.H., Gécz J. and Shoubridge C.
10. Molecular Pathology of Polyalanine Expansion Disorders-new
Perspectives from Mouse Models
James N Hughes and Paul Q Thomas
11. Yeast as a Platform to Explore Polyglutamine Toxicity and Aggregation
Martin L. Duennwald
12. Immunobased Detection Assays to Quantify Distinct Mutant Huntingtin Conformations in Biological Samples
Gregor P. Lotz and Andreas Weiss
13. Modeling and Analysis of Repeat RNA Toxicity in Drosophila
Samaraweera, S.E. , O'Keefe, L.V. , van Eyk, C.L. , Lawlor, K.T., Humphreys D.T., Suter, C.M. and Richards, R.I.
14. Analyzing Modifiers of Protein Aggregation in C. elegans by Native Agarose Gel Electrophoresis
Mats Holmberg, Ellen A.A. Nollen
15. Kinetic Analysis of Aggregation Data
Regina M. Murphy
16. A Bioinformatics Method for Identifying Q/N-rich Prion-like Domains in Proteins
Eric D. Ross, Kyle S. MacLea, Charles Anderson, and Asa Ben-Hur
17. Detecting Soluble PolyQ Oligomers and Investigating their Impact on Living Cells using Split-GFP
Patrick Lajoie and Erik Lee Snapp
18. Cell Biological Approaches To Investigate Polyglutamine-Expanded AR Metabolism
Lori J. Cooper and Diane E. Merry
Klappentext
The genomes of humans, as well as many other species, are interspersed with hundreds of thousands of tandem repeats of DNA sequences. Those tandem repeats located as codons within open reading frames encode amino acid runs, such as polyglutamine and polyalanine. Tandem repeats have not only been implicated in biological evolution, development and function but also in a large collection of human disorders. In Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols, expert researchers in the field detail many methods covering the analysis of tandem repeats in DNA, RNA and protein, in healthy and diseased states. This will include molecular genetics, molecular biology, biochemistry, proteomics, biophysics, cell biology, and molecular and cellular approaches to animal models of tandem repeat disorders. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.n nAuthoratative and Practical, Tandem Repeats in Genes, Proteins, and Disease: Methods and Protocols aids scientists in continuing to study the unique methodological challenges that come from repetitive DNA and poly-amino acid sequences.
Presents current up-to-date methodologies
n
Features tips on trouble shooting and avoiding known pitfalls
n
Contains key notes and implementation advice from the experts
n
