Recombinant Gene Expression
(Englisch)
Reviews and Protocols, Third Edition
Lorence, Argelia

32) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity

Lyne Jossé, Mark Smales, and Mick F. Tuite

33) High-throughput Baculovirus Expression in Insect Cells Richard B. Hitchman Robert D. Possee, and Linda A. King

34) Recombinant Protein Expression in Milk of Livestock Species

Zsuzsanna Bosze and Laszló Hiripi

Studies related to recombinant gene expression have brought new advance such as the emergence of the "omics" technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.

Using Folding Promoting Agents in Recombinant Protein Production: A Review.-Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass Spectrometry.-A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene Expression.-Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant Proteins.-Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli.-The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella.-Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble Fractions.-Heterologous Protein Expression by Lactococcus lactis.-An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview.-Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125.-A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125.-A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein Interaction.-Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatus.-Plasmid DNA Production for Therapeutic Applications.-Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A Review.-Expression of Multi-subunit Proteins in Leishmania tarentolae.-Recombinant Protein Production in Yeasts.-Yeasts as Tool for Heterologous Gene Expression.-The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal Endophytes.-Aptamer-regulated Expression of Essential Genes in Yeast.-Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastoris.-A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission.-Heterologous Gene Expression by Chromosomal Integration in Fission Yeast.-Genetic Engineering of Industrial Strains of Saccharomyces cerevisiae.-Recombinant Protein Production in Plants: Challenges and Solutions.-A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant Proteins.-Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy Roots.-Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion Technology.-Quality Assessment of Recombinant Proteins Produced in Plants.-Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins.-The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell Lines.-Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and Authenticity.-High-throughput Baculovirus Expression in Insect Cells.-Recombinant Protein Expression in Milk of Livestock Species.

Inhaltsverzeichnis

Table of Contents  ¿Recombinant Gene Expression: Reviews and Protocols, Third Edition¿Argelia Lorence (Editor) Name of corresponding author(s) is underlined General Aspects1)     Using Folding Promoting Agents in Recombinant Protein Production: A Review Beatrix Fahnert 2)    Routine Identity Confirmation of Recombinant Proteins by MALDI-TOF Mass SpectrometryBrett J. Savary and Prasanna Vasu 3)    A Matter of Packaging: Influence of Nucleosome Positioning on Heterologous Gene ExpressionMaría de la Cruz Muñoz-Centeno, Gonzalo Millán-Zambrano and Sebastián Chavez 4)    Tools of the Trade: Developing Antibody-Based Detection Capabilities for Recombinant ProteinsMaureen C. Dolan, Giuliana Medrano, Anthony McMickle, and Carole L. Cramer Prokaryotes 5)    Heat-shock Protein Fusion Vectors for Improved Expression of Soluble Recombinant Proteins in Escherichia coli Christos A. Kyratsous and Christos A. Panagiotidis 6)    The use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in SalmonellaFerenc Vonderviszt, Rachel Sajo, Jozsef Dobo, Peter Zavodoszky 7)    Optimization of Purification Protocols Based on the Step-by-step Monitoring of the Protein Aggregates in the Soluble FractionsArio De Marco  8)            Heterologous Protein Expression by Lactococcus lactisJulio Villatoro-Hernández, Oscar Paul Kuipers, Odila Saucedo-Cárdenas and Roberto Montes de Oca Luna 9)    An Extended Suite of Genetic Tools for Use in Bacteria of the Halomonadaceae: An Overview Monserrat  Argandoña, Carmen Vargas, Javier Rodríguez-Moya, Mercedes Reina-Bueno, Manuel Salvador and Joaquin J. Nieto 10)  Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125Valentina Rippa, Rosanna Papa, Maria Giuliani, Cinzia Pezzella, Ermenegilda Parrilli, Maria Luisa Tutino, Gennaro Marino, and Angela Duilio 11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella,Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino 12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein InteractionJumei Zeng and Zheng-Guo He 13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatusNadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper 14) Plasmid DNA Production for Therapeutic ApplicationsAlvaro Lara and Octavio T. Ramirez  Lower eukaryotes 15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A ReviewTomoaki Niimi  16) Expression of Multi-subunit Proteins in Leishmania tarentolae Marisa Sugino and Tomoaki Niimi Fungi 17) Recombinant Protein Production in YeastsDiethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro 18) Yeasts as Tool for Heterologous Gene ExpressionRaja Mokdad-Gargouri,  Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri 19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal EndophytesSimona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl20)  Aptamer-regulated Expression of Essential Genes in YeastBeatrix Suess, Karl-Dieter Entian, Peter Kötter and Julia E. Weigand 21) Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastorisPrasanna Vasu, Stefan Bauer, and Brett J. Savary  22) A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission Yeast Tiina Tamm 23) Heterologous Gene Expression by Chromosomal Integration in Fission YeastAkihisa Matsuyama and Minoru Yoshida 24) Genetic Engineering of Industrial Strains of Saccharomyces cerevisiaeSylvie Le Borgne Le Gall Plants and Plant Cells 25) Recombinant Protein Production in Plants: Challenges and SolutionsElizabeth E. Hood and Deborah V. Vicuña Requesens 26) A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant ProteinsJianfeng Xu and Marcia J. Kieliszewski27) Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy RootsLuis Ñopo, Bonnie J. Woffenden, Deborah Reed, Scott Buswell, Chenming Zhang,  and Fabricio Medina-Bolivar 28) Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion TechnologyJussi J Joensuu, Andrew J. Conley, Markus B. Linder, Rima Menassa 29) Quality Assessment of Recombinant Proteins Produced in Plants   Giuliana Medrano, Maureen C. Dolan, Jose Condori, David  N. Radin, and Carole L. Cramer 30) Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins Xumeng Ge and Jianfeng XuAnimals and Animal Cells 31) The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell LinesLeander Blaas, Monica Musteanu, Beatrice Grabner, Robert Eferl, Anton Bauer, and Emilio Casanova 32) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and AuthenticityLyne Jossé, Mark Smales, and Mick F. Tuite 33) High-throughput Baculovirus Expression in Insect Cells Richard B. Hitchman Robert D. Possee, and Linda A. King 34) Recombinant Protein Expression in Milk of Livestock SpeciesZsuzsanna B¿sze and Laszló Hiripi  11) A Novel Strategy for the Construction of Genomic Mutants of the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125Maria Giuliani, Ermenegilda Parrilli , Cinzia Pezzella,Valentina Rippa, Angela Duilio, Gennaro Marino and Maria Luisa Tutino 12) A New Bacterial Co-expression System for Over-expressing Soluble Protein and Validating Protein-protein InteractionJumei Zeng and Zheng-Guo He 13) Heterologous High-level Gene Expression in the Photosynthetic Bacterium Rhodobacter capsulatusNadine Katzke, René Bergmann, Karl-Erich Jaeger Thomas Drepper 14) Plasmid DNA Production for Therapeutic ApplicationsAlvaro Lara and Octavio T. Ramirez  Lower eukaryotes 15) Recombinant Protein Production in the Eukaryotic Protozoan Parasite Leishmania tarentolae: A ReviewTomoaki Niimi  16) Expression of Multi-subunit Proteins in Leishmania tarentolae Marisa Sugino and Tomoaki Niimi Fungi 17) Recombinant Protein Production in YeastsDiethard Mattanovich, Paola Branduardi, Laura Dato, Brigitte Gasser, Michael Sauer and Danilo Porro 18) Yeasts as Tool for Heterologous Gene ExpressionRaja Mokdad-Gargouri,  Salma Abdelmoula-Soussi, Nadia Hadiji-Abbès, Ines Yacoubi-Hadj Amor, Istabrak Borchani-Chabchoub and Ali Gargouri 19) The Cre/lox system: A Practical Tool to Efficiently Eliminate Selectable Markers in Fungal EndophytesSimona Florea, Caroline Machado, Kalina Andreeva, and Christopher L. Schardl20)  Aptamer-regulated Expression of Essential Genes in YeastBeatrix Suess, Karl-Dieter Entian, Peter Kötter and Julia E. Weigand 21) Cloning and Expression of Hemicellulases from Aspergillus nidulans in Pichia pastorisPrasanna Vasu, Stefan Bauer, and Brett J. Savary  22) A Thiamine-regulatable Epitope-tagged Protein Expression System in Fission Yeast Tiina Tamm 23) Heterologous Gene Expression by Chromosomal Integration in Fission YeastAkihisa Matsuyama and Minoru Yoshida 24) Genetic Engineering of Industrial Strains of Saccharomyces cerevisiaeSylvie Le Borgne Le Gall Plants and Plant Cells 25) Recombinant Protein Production in Plants: Challenges and SolutionsElizabeth E. Hood and Deborah V. Vicuña Requesens 26) A Novel Plant Cell Bioproduction Platform for High-yield Secretion of Recombinant ProteinsJianfeng Xu and Marcia J. Kieliszewski27) Super-promoter:TEV, a Powerful Gene Expression System for Tobacco Hairy RootsLuis Ñopo, Bonnie J. Woffenden, Deborah Reed, Scott Buswell, Chenming Zhang,  and Fabricio Medina-Bolivar 28) Bioseparation of Recombinant Proteins from Plant Extract with Hydrophobin Fusion TechnologyJussi J Joensuu, Andrew J. Conley, Markus B. Linder, Rima Menassa 29) Quality Assessment of Recombinant Proteins Produced in Plants   Giuliana Medrano, Maureen C. Dolan, Jose Condori, David  N. Radin, and Carole L. Cramer 30) Cell-free Protein Synthesis as a Promising Expression System for Recombinant Proteins Xumeng Ge and Jianfeng XuAnimals and Animal Cells 31) The use of Bacterial Artificial Chromosomes for Recombinant Protein Production in Mammalian Cell LinesLeander Blaas, Monica Musteanu, Beatrice Grabner, Robert Eferl, Anton Bauer, and Emilio Casanova 32) Engineering the Chaperone Network of CHO Cells for Optimal Recombinant Protein Production and AuthenticityLyne Jossé, Mark Smales, and Mick F. Tuite 33) High-throughput Baculovirus Expression in Insect Cells Richard B. Hitchman Robert D. Possee, and Linda A. King 34) Recombinant Protein Expression in Milk of Livestock SpeciesZsuzsanna B¿sze and Laszló Hiripi 

Klappentext

Studies related to recombinant gene expression have brought new advance such as  the emergence of the ¿omics¿ technologies. While Escherichia coli, Sacharomyces cerevisiae and insect cells continue to be the dominant production platforms of recombinant proteins. In Recombinant Gene Expression: Review and Protocols, Third Edition, expert researchers in the field detail many of the methods now commonly used to study recombinant gene expression. These include methods and techniques for bacteria, lower eukaryotes, fungi, plants and plant cells, and animals and animal cells. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.n nAuthoritative and practical, Recombinant Gene Expression: Review and Protocols, Third Edition seeks to aid scientists in the further study of this crucially important research into recombinant gene expression.

Contains new protocols and topics not yet covered

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras