Aids scientists in the further development and commercialization of microfluidic diganostic technologies
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Microfluidic Diagnostics: Methods in Molecular Biology seeks to aid scientists in the further development and commercialization of microfluidic diagnostic technologies
Section 1: Microfluidic Diagnostics: From the Classroom to the Boardroom
1. Present Technology and Future Trends in Point of Care Microfluidic Diagnostics
Lawrence Kulinsky, Zahra Noroozi, and Marc Madou
2. Teaching Microfluidic Diagnostics Using Jell-O Chips
Cheng Wei T. Yang and Eric T. Lagally
3. Fundamentals of Microfluidics for High School Students with No Prior Knowledge of Fluid Mechanics
Vishal Tandon and Walter Peck
4. Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method
Chenren Shao and Don L. DeVoe
5. Overview of the Microfluidic Diagnostics Commercial Landscape
L. Kim
6. Practical Aspects of the Preparation and Filing of Patent Applications
Fiona Bessoth
7. Introduction to In Vitro Diagnostic Device Regulatory Requirements
Jonathan Day
Section 2. Microfluidic Diagnostics: Fabrication and Manipulation Protocols
8. Microfluidic Device Fabrication by Thermoplastic Hot-Embossing
Shuang Yang and Don L. DeVoe
9. Introduction to Glass Microstructuring Techniques
Radoslaw Mazurczyk, Colin Mansfield, Marcin Lygan
10.Glass Microstructure Capping and Bonding Techniques
Radoslaw Mazurczyk, Colin Mansfield, and Marcin Lygan
11. Rapid Prototyping of PDMS Device using SU-8 Lithography
Gareth Jenkins
12. Microfluidic Interface Technology Based on Stereolithography for Glass-based Lab-on-a-Chips
Song-I Han and Ki-Ho Han
13. Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays
Scott T. Phillips and Nicole K. Thom
14. Thread Based Devices for Low-Cost Diagnostics
Meital Reches
15. Droplet Based Microfluidics
Sanjiv Sharma, Monphichar Srisa-Art, Steven Scott, Amit Asthana, and Anthony Cass
16. Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET
Monipchar Srisa-Art and Sanjiv Sharma
17. Surface Treatments for Microfluidic Biocompatibility
N.J. Shirtcliffe and P. Roach
18. Superhydrophobicity for Anti-Fouling Microfluidic Surfaces
N.J. Shirtcliffe, and P. Roach
Section 3. Microfluidic Diagnostics: Application Protocols
19. The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries
Samantha M. Hattersley, John Greenman, and Stephen J. Haswell
20. Applications of Microfluidics for Molecular Diagnostics
Harikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale
21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethysiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers
Peng Li
22. Breast Cancer Diagnostics using Microfluidic Multiplexed Immunohistochemistry
Minseok S. Kim, Seyong Kwon, and Je-Kyun Park
23. Charged-couple Device (CCD) Detectors for Lab-on-a Chip (LOC) Optical Analysis
Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck
24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate Hydrogels
Michael P. Cuchiara and Jennifer L. West
25. Purifications of DNA/RNA in a Microfluidic Device
Andy Fan, Samantha Byrnes, and Catherine Klapperich
26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR
Xuefei Leng and Chaoyong James Yang
27. Intergrated Fluidic Circuits (IFCs) for Digital PCR
Ramesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver
28. microFIND® Approach to Fluorescent in Situ Hybridization (FISH)
Andrea Zanardi, Emanuele Barborini, and Roberta Carbone
29. An ELISA Lab-on-a-CHOP (ElISA-LOC)
Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov
30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis
Eric Ouellet, Louise Lund, and Eric T. Lagally
31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement
Kerstin Länge, Friederike J. Gruhl, and Michael Rapp
32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins
Junjie Ou, Carolyn L. Ren
This Methods in Molecular Biology(TM) book covers microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methods from basic fabrication methods to commercializing research. Includes materials, protocols, tips and more.
Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical,
Microfluidic Diagnostics: Methods in Molecular Biology seeks to aid scientists in the further development and commercialization of microfluidic diagnostic technologies
Present Technology and Future Trends in Point of Care Microfluidic Diagnostics.-Teaching Microfluidic Diagnostics Using Jell-O® Chips.-Fundamentals of Microfluidics for High School Students with no Prior Knowledge of Fluid Mechanics.-Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring Method.-Overview of the Microfluidic Diagnostics Commercial Landscape.-Practical Aspects of the Preparation and Filing of Patent Applications.-Introduction to In Vitro Diagnostic Device Regulatory Requirements.-Microfluidic Device Fabrication by Thermoplastic Hot-Embossing.-Introduction to Glass Microstructuring Techniques.-Glass Microstructure Capping and Bonding Techniques.-Rapid Prototyping of PDMS Devices using SU 8 Lithography.-Microfluidic Interface Technology based on Stereolithography for Glass-based Lab-on-a-Chips.-Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays.-Thread Based Devices for Low-Cost Diagnostics.-Droplet Based Microfluidics.-Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRET.-Surface Treatments for Microfluidic Biocompatibility.-Superhydrophobicity for Anti-Fouling Microfluidic Surfaces.-The Application of Microfluidic Devices for Viral Diagnosis in Developing Countries.-Applications of Microfluidics for Molecular Diagnostics.-Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethylsiloxane Microfluidic Channels for Rapid Detection of Disease Biomarkers.-Breast Cancer Diagnostics using Microfluidic Multiplexed Immunohistochemistry.-Charged-coupled Device (CCD) Detectors for Lab-on-a chip (LOC) Optical Analysis.-Multilayer Microfluidic Poly(ethylene glycol)Diacrylate Hydrogels.-Purification of DNA/RNA in a Microfluidic Device.-Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR.-Integrated Fluidic Circuits (IFCs) for Digital PCR.-microFIND® Approach to Fluorescent in Situ Hybridization (FISH).-An ELISA Lab-on-a-Chip (ELISA-LOC).-Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker Analysis.-Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and Measurement.-Microchip UV Absorbance Detection Applied to Isoelectric Focusing of Proteins.
Inhaltsverzeichnis
Section 1: Microfluidic Diagnostics: From the Classroom to the Boardroom1. Present Technology and Future Trends in Point of Care Microfluidic DiagnosticsLawrence Kulinsky, Zahra Noroozi, and Marc Madou2. Teaching Microfluidic Diagnostics Using Jell-O ChipsCheng Wei T. Yang and Eric T. Lagally3. Fundamentals of Microfluidics for High School Students with No Prior Knowledge of Fluid MechanicsVishal Tandon and Walter Peck4. Measuring Microchannel Electroosmotic Mobility and Zeta Potential by the Current Monitoring MethodChenren Shao and Don L. DeVoe5. Overview of the Microfluidic Diagnostics Commercial LandscapeL. Kim 6. Practical Aspects of the Preparation and Filing of Patent Applications Fiona Bessoth7. Introduction to In Vitro Diagnostic Device Regulatory RequirementsJonathan DaySection 2. Microfluidic Diagnostics: Fabrication and Manipulation Protocols8. Microfluidic Device Fabrication by Thermoplastic Hot-EmbossingShuang Yang and Don L. DeVoe9. Introduction to Glass Microstructuring TechniquesRadoslaw Mazurczyk, Colin Mansfield, Marcin Lygan10.Glass Microstructure Capping and Bonding TechniquesRadoslaw Mazurczyk, Colin Mansfield, and Marcin Lygan11. Rapid Prototyping of PDMS Device using SU-8 LithographyGareth Jenkins12. Microfluidic Interface Technology Based on Stereolithography for Glass-based Lab-on-a-ChipsSong-I Han and Ki-Ho Han13. Three-Dimensional, Paper-Based Microfluidic Devices Containing Internal Timers for Running Time-Based Diagnostic Assays Scott T. Phillips and Nicole K. Thom14. Thread Based Devices for Low-Cost DiagnosticsMeital Reches15. Droplet Based MicrofluidicsSanjiv Sharma, Monphichar Srisa-Art, Steven Scott, Amit Asthana, and Anthony Cass16. Droplet-Based Microfluidics for Binding Assays and Kinetics Based on FRETMonipchar Srisa-Art and Sanjiv Sharma 17. Surface Treatments for Microfluidic BiocompatibilityN.J. Shirtcliffe and P. Roach18. Superhydrophobicity for Anti-Fouling Microfluidic SurfacesN.J. Shirtcliffe, and P. RoachSection 3. Microfluidic Diagnostics: Application Protocols 19. The Application of Microfluidic Devices for Viral Diagnosis in Developing CountriesSamantha M. Hattersley, John Greenman, and Stephen J. Haswell20. Applications of Microfluidics for Molecular DiagnosticsHarikrishnan Jayamohan, Himanshu J. Sant, and Bruce K. Gale21. Quantitative Heterogeneous Immunoassays in Protein Modified Polydimethysiloxane Microfluidic Channels for Rapid Detection of Disease BiomarkersPeng Li22. Breast Cancer Diagnostics using Microfluidic Multiplexed ImmunohistochemistryMinseok S. Kim, Seyong Kwon, and Je-Kyun Park23. Charged-couple Device (CCD) Detectors for Lab-on-a Chip (LOC) Optical Analysis Avraham Rasooly, Yordan Kostov, and Hugh A. Bruck24. Multilayer Microfluidic Poly(ethylene glycol) Diacrylate HydrogelsMichael P. Cuchiara and Jennifer L. West25. Purifications of DNA/RNA in a Microfluidic DeviceAndy Fan, Samantha Byrnes, and Catherine Klapperich26. Agarose Droplet Microfluidics for Highly Parallel and Efficient Single Molecule Emulsion PCR Xuefei Leng and Chaoyong James Yang27. Intergrated Fluidic Circuits (IFCs) for Digital PCRRamesh Ramakrishnan, Jian Qin, Robert C. Jones, and Lesley S. Weaver28. microFIND® Approach to Fluorescent in Situ Hybridization (FISH) Andrea Zanardi, Emanuele Barborini, and Roberta Carbone29. An ELISA Lab-on-a-CHOP (ElISA-LOC)Avraham Rasooly, Hugh A. Bruck, and Yordan Kostov30. Multiplexed Surface Plasmon Resonance Imaging for Protein Biomarker AnalysisEric Ouellet, Louise Lund, and Eric T. Lagally31. Surface Acoustic Wave (SAW) Biosensors: Coupling of Sensing Layers and MeasurementKerstin Länge, Friederike J. Gruhl, and Michael Rapp32. Microchip UV Absorbance Detection Applied to Isoelectric Focusing of ProteinsJunjie Ou, Carolyn L. Ren
Klappentext
Microfluidic techniques are becoming widely incorporated into medical diagnostic systems due to the inherent advantages of miniaturization. In Microfluidic Diagnostics: Methods in Molecular Biology, researchers in the field detail methods and protocols covering subjects such as microfluidic device fabrication, on-chip sample preparation, diagnostic applications and detection methodologies. The protocols described range from cutting-edge developments to established techniques and basic demonstrations suitable for education and training; from basic fabrication methods to commercializing research. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.n Authoritative and practical, Microfluidic Diagnostics: Methods in Molecular Biology seeks to aid scientists in the further development and commercialization of microfluidic diagnostic technologies
Aids scientists in the further development and commercialization of microfluidic diganostic technologies
n
Provides step-by-step detail essential for reproducible results
n
Contains key notes and implementation advice from the experts
n
Includes supplementary material: sn.pub/extras
n
