Features methods involving a wide variety of tissue types in one convenient volume
Written by leading experts in an easy-to-use step-by-step format with notes providing practical, straight-from-the-lab knowledge and experiences
Covers protocols with fetal tissues and stem cells as well as cultures of isolated cell types for the study of developmental processes and homogeneous cell populations, respectively
1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid BodiesMelany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) CulturesAlastair S. Garfield3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem CellsJanet Smith and Deborah Merrick4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA InterferenceJamie A. Davies5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular DifferentiationPierre-Marie del Moral and David Warburton6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and IntestineJonathan M. Quinlan, Wei-Yuan Yu, and David Tosh7. Isolation and Culture of Embryonic Pancreas and LiverZoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh8. Isolation and Culture of Mouse Satellite CellsAntonio Musarò and Laura Barberi9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte CulturesNigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine KeratinocytesReto Caldelari and Eliane J. Müller11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial CellsMatthew J. Smalley12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent ProbesMagalie A. Ravier and Guy A. Rutter13. Isolation and Culture of Adult Mouse HepatocytesWan-ChunLi, Kate L. Ralphs, and David Tosh14. Isolation and Culture of Mouse Intestinal CellsF. Charles Campbell15. Derivation of Primary Choroid Plexus Epithelial Cells from the MouseTrevelyan R. Menheniott, Marika Charalambous, and Andrew Ward16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent CytochemistryCarla Sciarretta and Liliana Minichiello17. Cell Culture of Primary Cerebellar Granule CellsDana Krämer and Liliana Minichiello18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse BrainHenrik Ahlenius and Zaal Kokaia
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
From the reviews:
"This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. ... This book is recommended for researchers and students who are interested in studying the culture of cells from animal models.” (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)
In this book, expert researchers provide a number of methods for the culture of a range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Notes provide practical, straight-from-the-lab knowledge and experiences.
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies.- Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures.- Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells.- The Embryonic Kidney: Isolation, Organ Culture, Immunostaining and RNA Interference.- Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium, or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation.- Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine.- Isolation and Culture of Embryonic Pancreas and Liver.- Isolation and Culture of Mouse Satellite Cells.- Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures.- Short- and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes.- Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells.- Isolation and Culture of Mouse Pancreatic Islets for Ex Vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes.- Isolation and Culture of Adult Mouse Hepatocytes.- Isolation and Culture of Mouse Intestinal Cells.- Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse.- The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry.- Cell Culture of Primary Cerebellar Granule Cells.- Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain.
From the reviews:
"This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. ... This book is recommended for researchers and students who are interested in studying the culture of cells from animal models." (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)
Inhaltsverzeichnis
1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies
Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester
2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures
Alastair S. Garfield
3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem Cells
Janet Smith and Deborah Merrick
4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA Interference
Jamie A. Davies
5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation
Pierre-Marie del Moral and David Warburton
6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine
Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh
7. Isolation and Culture of Embryonic Pancreas and Liver
Zoë D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh
8. Isolation and Culture of Mouse Satellite Cells
Antonio Musarò and Laura Barberi
9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures
Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton
10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes
Reto Caldelari and Eliane J. Müller
11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial Cells
Matthew J. Smalley
12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes
Magalie A. Ravier and Guy A. Rutter
13. Isolation and Culture of Adult Mouse Hepatocytes
Wan-ChunLi, Kate L. Ralphs, and David Tosh
14. Isolation and Culture of Mouse Intestinal Cells
F. Charles Campbell
15. Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse
Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward
16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry
Carla Sciarretta and Liliana Minichiello
17. Cell Culture of Primary Cerebellar Granule Cells
Dana Krämer and Liliana Minichiello
18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Henrik Ahlenius and Zaal Kokaia
Klappentext
Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.nnPractical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.
Features methods involving a wide variety of tissue types in one convenient volume
Written by leading experts in an easy-to-use step-by-step format with notes providing practical, straight-from-the-lab knowledge and experiences
Covers protocols with fetal tissues and stem cells as well as cultures of isolated cell types for the study of developmental processes and homogeneous cell populations, respectively
Includes supplementary material: sn.pub/extras
