Features cutting-edge methods and protocols examining genes and gene interactions in legumes
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, tranillegalscriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and useful,
Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
1. The Model Legume Genomes
Steven B. Cannon
2. Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization
René Geurts and Hans de Jong
3. Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes
Shaun J. Curtin, Justin E. Anderson, Colby G. Starker, Nicholas J. Baltes, Dhananjay Mani, Daniel F. Voytas, and Robert M. Stupar
4. RNA-Seq for Tranillegalscriptome Analysis in Non-Model Plants
Rohini Garg and Mukesh Jain
5. Functional Analysis of Legume Genome Arrays
Nicolas Goffard and Georg Weiller
6. Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing
Tian-Zuo Wang and Wen-Hao Zhang
7. Determining Abundance of MicroRNAs and Other Small RNAs in Legumes
Cecilia Contreras-Cubas, Alejandra A. Covarrubias, and José Luis Reyes
8. Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines
Juan Carlos Serrani Yarce, Hee-Kyung Lee, Million Tadege, Pascal Ratet, and Kirankumar S. Mysore
9. Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection
Maria Carelli, Ornella Calderini, Francesco Panara, Andrea Porceddu , Ilaria Losini, Pietro Piffanelli, Sergio Arcioni, and Carla Scotti
10. High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants
Dorian Fabian Urbanski, Anna Malolepszy, Jens Stougaard, and Stig Uggerhøj Andersen
11. Isolation and Forward Genetic Analysis of Developmental Genes in Pea
James L. Weller, Valérie F.G. Hecht, and Frances C. Sussmilch
12. Gene Silencing in Medicago truncatula Roots Using RNAi
Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, and Maria J. Harrison
13. Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)
Islam Shahidul, Huaan Yang, and Guijun Yan
14. Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens
Youhong Song, Kim E. Nolan, and Ray J. Rose
15. Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration
Kelsey Picard, Robyn Lee, Roger Hellens, and Richard C. Macknight
16. Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins
David A. Collings
17. Proteomics and the Analysis of Nodulation
Nijat Imin
18. Phosphoproteomic Analysis of Peptides
Mark A. Baker
19. Plant Metabolomics: From Experimental Design to Knowledge Extraction
Amit Rai, Shivshankar Umashankar, and Sanjay Swarup
This book features cutting-edge methods and protocols used to examine genes and gene interactions in legumes. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, tranillegalscriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and useful,
Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
The Model Legume Genomes.- Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization.- Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes.- RNA-Seq for Tranillegalscriptome Analysis in Non-Model Plants.- Functional Analysis of Legume Genome Arrays.- Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing.- Determining Abundance of MicroRNAs and Other Small RNAs in Legumes.- Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines.- Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection.- High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants.- Isolation and Forward Genetic Analysis of Developmental Genes in Pea.- Gene Silencing in Medicago truncatula Roots Using RNAi.- Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius).- Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens.- Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration.- Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins.- Proteomics and the Analysis of Nodulation.- Phosphoproteomic Analysis of Peptides.- Plant Metabolomics: From Experimental Design to Knowledge Extraction.
Inhaltsverzeichnis
1. The Model Legume Genomes
Steven B. Cannon
2. Fluorescent In Situ Hybridization (FISH) on Pachytene Chromosomes as a Tool for Genome Characterization
René Geurts and Hans de Jong
3. Targeted Mutagenesis for Functional Analysis of Gene Duplication in Legumes
Shaun J. Curtin, Justin E. Anderson, Colby G. Starker, Nicholas J. Baltes, Dhananjay Mani, Daniel F. Voytas, and Robert M. Stupar
4. RNA-Seq for Tranillegalscriptome Analysis in Non-Model Plants
Rohini Garg and Mukesh Jain
5. Functional Analysis of Legume Genome Arrays
Nicolas Goffard and Georg Weiller
6. Genome-Wide Identification of MicroRNAs in Medicago truncatula by High-Throughput Sequencing
Tian-Zuo Wang and Wen-Hao Zhang
7. Determining Abundance of MicroRNAs and Other Small RNAs in Legumes
Cecilia Contreras-Cubas, Alejandra A. Covarrubias, and José Luis Reyes
8. Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines
Juan Carlos Serrani Yarce, Hee-Kyung Lee, Million Tadege, Pascal Ratet, and Kirankumar S. Mysore
9. Reverse Genetics in Medicago truncatula Using a TILLING Mutant Collection
Maria Carelli, Ornella Calderini, Francesco Panara, Andrea Porceddu , Ilaria Losini, Pietro Piffanelli, Sergio Arcioni, and Carla Scotti
10. High-Throughput and Targeted Genotyping of Lotus japonicus LORE1 Insertion Mutants
Dorian Fabian Urbanski, Anna Malolepszy, Jens Stougaard, and Stig Uggerhøj Andersen
11. Isolation and Forward Genetic Analysis of Developmental Genes in Pea
James L. Weller, Valérie F.G. Hecht, and Frances C. Sussmilch
12. Gene Silencing in Medicago truncatula Roots Using RNAi
Daniela S. Floss, Alexa M. Schmitz, Colby G. Starker, J. Stephen Gantt, and Maria J. Harrison
13. Molecular Markers for Genetics and Plant Breeding: The MFLP Marker System and Its Application in Narrow-Leafed Lupin (Lupinus angustifolius)
Islam Shahidul, Huaan Yang, and Guijun Yan
14. Stable Transformation of Medicago truncatula cv. Jemalong for Gene Analysis Using Agrobacterium tumefaciens
Youhong Song, Kim E. Nolan, and Ray J. Rose
15. Transient Gene Expression in Medicago truncatula Leaves Via Agroinfiltration
Kelsey Picard, Robyn Lee, Roger Hellens, and Richard C. Macknight
16. Subcellular Localization of Transiently-Expressed Fluorescent Fusion Proteins
David A. Collings
17. Proteomics and the Analysis of Nodulation
Nijat Imin
18. Phosphoproteomic Analysis of Peptides
Mark A. Baker
19. Plant Metabolomics: From Experimental Design to Knowledge Extraction
Amit Rai, Shivshankar Umashankar, and Sanjay Swarup
Klappentext
Featuring current resources used to discover new legume family genes and to understand genes and their interactions, Legume Genomics: Methods and Protocols provides techniques from expert researchers to study these plants that are so vitally important for food, feed, human nutrition, bioenergy, and industrial purposes. This detailed volume covers genome characterization and analysis, tranillegalscriptome analysis and miRNA identification/analysis, forward and reverse genetics, molecular markers, as well as transformation strategies used to investigate gene function and many other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.n Authoritative and useful, Legume Genomics: Methods and Protocols aims to serve plant molecular biologists, molecular breeders, plant physiologists and biochemists, developmental biologists, and those interested in plant-microbe interactions.
Features cutting-edge methods and protocols examining genes and gene interactions in legumes
rn
Provides step-by-step detail essential for reproducible results
rn
Contains key notes and implementation advice from the experts
rn
