Fully updated with cutting-edge additions to the popular first edition
Provides step-by-step reproducible laboratory protocols
Contains tips on troubleshooting and avoiding known pitfalls
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.
1. Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues
Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
2. Detecting p53 Isoforms at Protein Level
Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
3. Autophagy, Senescence and Apoptosis
Rachel W. Goehe, Molly L. Bristol, Eden N. Wilson, and David A. Gewirtz
4. p53 and Cell Cycle Effects after DNA Damage
Emir Senturk, James J. Manfredi
5. p53 Ubiquitination and Proteasomal Degradation
Ian Love, Dingding Shi, Steven R. Grossman
6. Identification of p53 in Mitochondria
Angelina V. Vaseva and Ute M. Moll
7. Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis
Sumitra Deb and Paul R. Graves
8. Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization
Qi Zhang, and Hua Lu
9. Determine the Effect of p53 on Chemosensitivity
Emir Senturk, James J. Manfredi
10. Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells
Mahesh Ramamoorthy, Catherine Vaughan, Sumitra Deb, Swati Palit Deb
11. Mutant p53 in Cell Adhesion and Motility
W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb
12. Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication
Rebecca A. Frum, Sumitra Deb and Swati Palit Deb
13. Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts
Evguenia M. Alexandrova and Ute M. Moll
14. p53 Actions on MicroRNA Expression and Maturation Pathway
Hiroshi I. Suzuki and Kohei Miyazono
15. Isolation and Characterization of Murine Multipotent Lung Stem Cells
Venkat S Gadepalli, Catherine A Vaughan, Raj R. Rao
16. Generation of p53 Knock-down Cell Lines
Catherine Vaughan, Swati Palit Deb, Sumitra Deb
17. ChIP for Identification of p53 Responsive DNA Promoters
Jun-Ming Liao and Hua Lu
18. ChIP-on-chip to Identify Mutant p53 Targets
Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino
19. ChIP Sequencing to Identify p53 Targets
Catherine Vaughan, Brad Windle, Sumitra Deb
20. The p53-Mdm2 Loop: A Critical Juncture of Stress Response
Yaara Levav-Cohen, Zehavit Goldberg, Valentina Zuckerman, Sue Haupt, and Ygal Haupt
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition.
In its updated 2nd edition, this Methods in Molecular Biology(TM) book offers tools for identifying target genes, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, generation of pluripotent stem cells and more.
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.
Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues.- Detecting p53 Isoforms at Protein Level.- Autophagy, Senescence and Apoptosis.- p53 and Cell Cycle Effects after DNA Damage.- p53 Ubiquitination and Proteasomal Degradation.-Identification of p53 in Mitochondria.-Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis.-Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization.- Determine the Effect of p53 on Chemosensitivity.-Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells.- Mutant p53 in Cell Adhesion and Motility.- Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication.- Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts.- p53 Actions on MicroRNA Expression and Maturation Pathway.- Isolation and Characterization of Murine Multipotent Lung Stem Cells.- Generation of p53 Knock-down Cell Lines.-ChIP for Identification of p53 Responsive DNA Promoters.- ChIP-on-chip to Identify Mutant p53 Targets.- ChIP Sequencing to Identify p53 Targets.- The p53-Mdm2 Loop: A Critical Juncture of Stress Response.
Inhaltsverzeichnis
1. Detecting and Quantifying p53 Isoforms at mRNA Level in Cell Lines and Tissues
Marie P. Khoury, Virginie Marcel, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
2. Detecting p53 Isoforms at Protein Level
Virginie Marcel, Marie P. Khoury, Kenneth Fernandes, Alexandra Diot, David P. Lane and Jean-Christophe Bourdon
3. Autophagy, Senescence and Apoptosis
Rachel W. Goehe, Molly L. Bristol, Eden N. Wilson, and David A. Gewirtz
4. p53 and Cell Cycle Effects after DNA Damage
Emir Senturk, James J. Manfredi
5. p53 Ubiquitination and Proteasomal Degradation
Ian Love, Dingding Shi, Steven R. Grossman
6. Identification of p53 in Mitochondria
Angelina V. Vaseva and Ute M. Moll
7. Identification of Novel Mutant p53 Interacting Proteins by Proteomic Analysis
Sumitra Deb and Paul R. Graves
8. Identification of Small Molecules Affecting p53-MDM2/MDMX Interaction by Fluorescence Polarization
Qi Zhang, and Hua Lu
9. Determine the Effect of p53 on Chemosensitivity
Emir Senturk, James J. Manfredi
10. Measurement of Chemosensitivity and Growth Rate in p53 Expressing Cells
Mahesh Ramamoorthy, Catherine Vaughan, Sumitra Deb, Swati Palit Deb
11. Mutant p53 in Cell Adhesion and Motility
W. Andrew Yeudall, Katharine H. Wrighton, and Sumitra Deb
12. Use of the DNA Fiber Spreading Technique to Detect the Effects of Mutant p53 on DNA Replication
Rebecca A. Frum, Sumitra Deb and Swati Palit Deb
13. Generation of p53-deficient Induced Pluripotent Stem Cells (iPS) from Mouse Embryo Fibroblasts
Evguenia M. Alexandrova and Ute M. Moll
14. p53 Actions on MicroRNA Expression and Maturation Pathway
Hiroshi I. Suzuki and Kohei Miyazono
15. Isolation and Characterization of Murine Multipotent Lung Stem Cells
Venkat S Gadepalli, Catherine A Vaughan, Raj R. Rao
16. Generation of p53 Knock-down Cell Lines
Catherine Vaughan, Swati Palit Deb, Sumitra Deb
17. ChIP for Identification of p53 Responsive DNA Promoters
Jun-Ming Liao and Hua Lu
18. ChIP-on-chip to Identify Mutant p53 Targets
Frauke Goeman, Giulia Fontemaggi, and Giovanni Blandino
19. ChIP Sequencing to Identify p53 Targets
Catherine Vaughan, Brad Windle, Sumitra Deb
20. The p53-Mdm2 Loop: A Critical Juncture of Stress Response
Yaara Levav-Cohen, Zehavit Goldberg, Valentina Zuckerman, Sue Haupt, and Ygal Haupt
Klappentext
The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition. In p53 Protocols, Second Edition expert researchers in the field detail many of the methods that address the challenging questions of the p53 field. These methods include the identification of the target genes and binding partners of gain of function p53 mutants, methods to determine stress response, autophagy or senescence induced by p53, cell cycle analysis, analysis of different phases of DNA replication modified by p53, and generation of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.n nAuthoritative and practical, p53 Protocols, Second Edition seeks to aid scientists in the further study into p53 and other tumor suppressors.
Fully updated with cutting-edge additions to the popular first edition
Provides step-by-step reproducible laboratory protocols
Contains tips on troubleshooting and avoiding known pitfalls
Includes supplementary material: sn.pub/extras
