Includes cutting-edge methods and protocols involving dopamine receptor research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols involving dopamine receptor research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-tranillegalscriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful.
Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.
Part I: Genetic, Epigenetic, and Post-Tranillegalscriptional Analysis of Dopamine Receptors
1. Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene
Laura M. Fiori, Mireille Daigle, and Paul R. Albert
2. Characterization of D1 Dopamine Receptor Post-Tranillegalscriptional Regulation
Eldo V. Kuzhikandathil
Part II: Computational and Biochemical Methods in the Investigation of Dopamine Receptor Structure, Binding, and Post-Translational Regulation
3. Computational Approaches in the Structure-Function Studies of Dopamine Receptors
Mayako Michino and Lei Shi
4. Cell-Free Protein Synthesis and Purification of the Dopamine D2 Receptor
Dipannita Basu, Ritesh Daya, Christal D.R. Sookram, and Ram K. Mishra
5. Wnt Ligand Binding to and Regulation of Dopamine D2 Receptors
Sehyoun Yoon, Mi-Hyun Choi, and Ja-Hyun Baik
6. Regulation of Pre- and Postsynaptic Protein Phosphorylation by Dopamine D2 Receptors
Alessandra Bonito-Oliva, Giada Spigolon, and Gilberto Fisone
7. Study of Dopamine D1 Receptor Regulation by G Protein-Coupled Receptor Kinases Using Whole-Cell Phosphorylation and Cross-Linking Methods
Keyvan Sedaghat, Boyang Zhang, Xiaodi Yang, Caroline Lefebvre, and Mario Tiberi
8. Ubiquitination of Dopamine Receptor Studied by Sequential Double Immunoprecipitation
Kamila Skieterska, Pieter Rondou, and Kathleen Van Craenenbroeck
Part III: Visualization and Imaging of Dopamine Receptors and Ligands
9. Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors
Lionel Froux, Diana Suarez-Boomgaard, Jerome Baufreton, Alicia Rivera, Maurice Garret, and Anne Taupignon
10. MALDI Mass Spectrometry Imaging of Dopamine and PET D1 and D2 Receptor Ligands in Rodent Brain Tissues
Richard J.A. Goodwin, Mohammadreza Shariatgorji, and Per E. Andren
11. Positron Emission Tomography Imaging of Dopaminergic Receptors in Rats
Boguslaw Szczupak and Abraham Martín
Part IV: Molecular and Cell Biological Approaches in the Study of Dopamine Receptor Function
12. Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors
Jeff S. Kruk, Azita Kouchmeshky, Nicholas Grimberg, Marina Rezkella, and Michael A. Beazely
13. Dopamine Receptors in Human Embryonic Stem Cell Differentiation
Glenn S. Belinsky, Mandakini B. Singh, Katerina D. Oikonomou, Michele L. McGovern, and Srdjan D. Antic
14. Calcium and PLC Signaling Through Dopamine Receptors
Lani S. Chun, R. Benjamin Free, and David R. Sibley
15. Intracellular Trafficking Assays for Dopamine D2-Like Receptors
Chengchun Min, Mei Zheng, and Kyeong-Man Kim
16. Study of Crosstalk Between Dopamine Receptors and Ion Channels
Ping Su, Albert H.C. Wong, and Fang Liu
Part V: Behavioral Analysis of Dopamine Function
17. Study of Dopamine Receptor and Dopamine Transporter Networks in Mice
Victor Gorgievski, Eleni T. Tzavara, and Bruno Giros
18. Optogenetic Regulation of Dopamine Receptor-Expressing Neurons
T. Chase Francis and Mary Kay Lobo
19. Characterization of D3 Dopamine Receptor Agonist-Dependent Tolerance Property
Samantha R. Cote and Eldo V. Kuzhikandathil
20. Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography
Dariusz Žurawek, Agata Faron-Górecka, Maciej Kusmider, Joanna Solich, Magdalena Kolasa, Kinga Szafran, Katarzyna Kmiotek, Piotr Gruca, Mariusz Papp, and Marta Dziedzicka-Wasylewska
Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene.- Characterization of D1 Dopamine Receptor Post-Tranillegalscriptional Regulation.- Computational Approaches in the Structure-Function Studies of Dopamine Receptors.- Cell-Free Protein Synthesis and Purification of the Dopamine D2 Receptor.- Wnt Ligand Binding to and Regulation of Dopamine D2 Receptors.- Regulation of Pre- and Postsynaptic Protein Phosphorylation by Dopamine D2 Receptors.- Study of Dopamine D1 Receptor Regulation by G Protein-Coupled Receptor Kinases Using Whole-Cell Phosphorylation and Cross-Linking Methods.- Ubiquitination of Dopamine Receptor Studied by Sequential Double Immunoprecipitation.- Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors.- MALDI Mass Spectrometry Imaging of Dopamine and PET D1 and D2 Receptor Ligands in Rodent Brain Tissues.- Positron Emission Tomography Imaging of Dopaminergic Receptors in Rats.- Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors.- Dopamine Receptors in Human Embryonic Stem Cell Differentiation.- Calcium and PLC Signaling Through Dopamine Receptors.- Intracellular Trafficking Assays for Dopamine D2-Like Receptors.- Study of Crosstalk Between Dopamine Receptors and Ion Channels.- Study of Dopamine Receptor and Dopamine Transporter Networks in Mice.- Optogenetic Regulation of Dopamine Receptor-Expressing Neurons.- Characterization of D3 Dopamine Receptor Agonist-Dependent Tolerance Property.- Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography.
Inhaltsverzeichnis
Part I: Genetic, Epigenetic, and Post-Tranillegalscriptional Analysis of Dopamine Receptors
1. Genetic and Epigenetic Methods for Analysis of the Dopamine D2 Receptor Gene
Laura M. Fiori, Mireille Daigle, and Paul R. Albert
2. Characterization of D1 Dopamine Receptor Post-Tranillegalscriptional Regulation
Eldo V. Kuzhikandathil
Part II: Computational and Biochemical Methods in the Investigation of Dopamine Receptor Structure, Binding, and Post-Translational Regulation
3. Computational Approaches in the Structure-Function Studies of Dopamine Receptors
Mayako Michino and Lei Shi
4. Cell-Free Protein Synthesis and Purification of the Dopamine D2 Receptor
Dipannita Basu, Ritesh Daya, Christal D.R. Sookram, and Ram K. Mishra
5. Wnt Ligand Binding to and Regulation of Dopamine D2 Receptors
Sehyoun Yoon, Mi-Hyun Choi, and Ja-Hyun Baik
6. Regulation of Pre- and Postsynaptic Protein Phosphorylation by Dopamine D2 Receptors
Alessandra Bonito-Oliva, Giada Spigolon, and Gilberto Fisone
7. Study of Dopamine D1 Receptor Regulation by G Protein-Coupled Receptor Kinases Using Whole-Cell Phosphorylation and Cross-Linking Methods
Keyvan Sedaghat, Boyang Zhang, Xiaodi Yang, Caroline Lefebvre, and Mario Tiberi
8. Ubiquitination of Dopamine Receptor Studied by Sequential Double Immunoprecipitation
Kamila Skieterska, Pieter Rondou, and Kathleen Van Craenenbroeck
Part III: Visualization and Imaging of Dopamine Receptors and Ligands
9. Dopamine Receptors in the Subthalamic Nucleus: Identification and Localization of D5 Receptors
Lionel Froux, Diana Suarez-Boomgaard, Jerome Baufreton, Alicia Rivera, Maurice Garret, and Anne Taupignon
10. MALDI Mass Spectrometry Imaging of Dopamine and PET D1 and D2 Receptor Ligands in Rodent Brain Tissues
Richard J.A. Goodwin, Mohammadreza Shariatgorji, and Per E. Andren
11. Positron Emission Tomography Imaging of Dopaminergic Receptors in Rats
Boguslaw Szczupak and Abraham Martín
Part IV: Molecular and Cell Biological Approaches in the Study of Dopamine Receptor Function
12. Transactivation of Receptor Tyrosine Kinases by Dopamine Receptors
Jeff S. Kruk, Azita Kouchmeshky, Nicholas Grimberg, Marina Rezkella, and Michael A. Beazely
13. Dopamine Receptors in Human Embryonic Stem Cell Differentiation
Glenn S. Belinsky, Mandakini B. Singh, Katerina D. Oikonomou, Michele L. McGovern, and Srdjan D. Antic
14. Calcium and PLC Signaling Through Dopamine Receptors
Lani S. Chun, R. Benjamin Free, and David R. Sibley
15. Intracellular Trafficking Assays for Dopamine D2-Like Receptors
Chengchun Min, Mei Zheng, and Kyeong-Man Kim
16. Study of Crosstalk Between Dopamine Receptors and Ion Channels
Ping Su, Albert H.C. Wong, and Fang Liu
Part V: Behavioral Analysis of Dopamine Function
17. Study of Dopamine Receptor and Dopamine Transporter Networks in Mice
Victor Gorgievski, Eleni T. Tzavara, and Bruno Giros
18. Optogenetic Regulation of Dopamine Receptor-Expressing Neurons
T. Chase Francis and Mary Kay Lobo
19. Characterization of D3 Dopamine Receptor Agonist-Dependent Tolerance Property
Samantha R. Cote and Eldo V. Kuzhikandathil
20. Dopamine D1 and D2 Receptors in Chronic Mild Stress: Analysis of Dynamic Receptor Changes in an Animal Model of Depression Using In Situ Hybridization and Autoradiography
Dariusz Zurawek, Agata Faron-Górecka, Maciej Kusmider, Joanna Solich, Magdalena Kolasa, Kinga Szafran, Katarzyna Kmiotek, Piotr Gruca, Mariusz Papp, and Marta Dziedzicka-Wasylewska
Klappentext
To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers. Divided in five sections dedicated to experimental approaches investigating different facets of dopaminergic signal transduction, Dopamine Receptor Technologies covers epigenetic and post-tranillegalscriptional analysis, computational and biochemical techniques, visualization and imaging methods, molecular and cell biological tools, as well as behavioral assessment. The book, as a part of the popular Neuromethods series, provides insightful step-by-step protocols and methodological reviews that readers will find useful.
Practical and versatile, Dopamine Receptor Technologies seeks to aid researchers in developing new pharmacological tools to improve our knowledge of in vivo roles played by each receptor subtype and the synthesis of prospective lead compounds for drug discovery.
Includes cutting-edge methods and protocols involving dopamine receptor research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
