Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.
PART I- Introducing Nucleic Acid Testing In The Veterinary Laboratory
1. Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory
Mónica V. Cunha And João Inácio
2. Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice
Alicia Aranaz
3. Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory
Joseph Kozlovac And Beverly Schmitt
4. Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes
Tina Lombardo, Silvia Dotti, Riccardo Villa, Stefano Cinotti, And Maura Ferrari
5. Biological Specimen Collection And Processing for Molecular Analysis
Susan Sanchez And Holly Sellers
6. Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory
Nick Saunders and Ian Sharp
PART II - Molecular Detection and Identification of Animal Pathogens in Laboratorial Settings
7. Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals
Fredrik Granberg, Oskar Karlsson, Mikael Leijon, Lihong Liu, and Sándor Belák
8. Real Time Reverse Tranillegalscriptase PCR For The Detection Of Bluetongue Virus
Carrie Batten, Lorraine Frost, And Chris Oura
9. Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues
Pedro Costa, Isabel Couto, Miguel Viveiros, and João Inácio
10. A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants
Florian Roeber, Aaron R. Jex, And Robin B. Gasser
11. Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches
Irene R. Grant And Linda D. Stewart
12. Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique
Hatem Soliman, Mona Saleh, And Mansour El-Matbouli
13. Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches
Jacinto Gomes And João Inácio
14. Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection
Ana Hurtado
15. DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp.
Christiane Schnee and Konrad Sachse
16. In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues
Maria Ballester and Fernando Rodriguez
17. Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections
Henrik Elvang Jensen, Louise Kruse Jensen, Kristiane Barington, Susanne Pors, Thomas Bjarnsholt, and Mette Boye
18. Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry
Joachim Frey and Peter Kuhnert
19. Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases
Mona Saleh, Hatem Soliman, and Mansour El-Matbouli
20. Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of Animal Pathogens
Fernando Teles And Luís Pina Fonseca
PART III - Molecular Profiling Of Veterinary Relevant Microbial Pathogens
21. Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms
Konrad Sachse and Petra Moebius
22. Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis
Ana Cláudia Carreira and Mónica V. Cunha
23. Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci
Ilda Santos-Sanches, Lélia Chambel, and Rogério Tenreiro
24. Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species
Giuliano Garofolo
25. Multilocus Sequence Typing (MLST): Markers For The Traceability Of Pathogenic Leptospira Strains
Ahmed Ahmed, Ana S. Ferreira, and Rudy A. Hartskeerl
26. Single Nucleotide Polymorphisms Discrimination Using High-Resolution Melting Analysis For The Genotyping Of Bacillus Anthracis
Sylviane Derzelle
27. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Analysis Of Members Of The Mycobacterium Tuberculosis Complex
Ana Botelho, Ana Canto, Célia Leão, and Mónica V. Cunha
28. Rapid Microarray-Based Genotyping Of Chlamydia Spp. Strains From Clinical Tissue Samples
Konrad Sachse and Anke Ruettger
29. Multiplexed Genotyping Of Bacillus Anthracis By Luminex Xmap Suspension Array
Simon Thierry and Sylviane Derzelle
PART IV - Integrative Omics and High-Throughput Platforms to Unravel the Biology of Pathogens and Their Interaction With the Host
30. Next-Generation Sequencing In Veterinary Medicine: How Can The Massive Amount Of Information Arising From High-Throughput Technologies Improve Diagnosis, Control and Management Of Infectious Diseases?
Steven Van Borm, Sándor Belák, Graham Freimanis, Alice Fusaro, Fredrik Granberg, Dirk Höper, Donald P. King, Isabella Monne, Richard Orton, and Toon Rosseel
31. Impact Of Next-Generation Technologies On Exploring Socio-Economically Important Parasites And Developing New Interventions
Cinzia Cantacessi, Andreas Hofmann, Bronwyn E. Campbell, and Robin B. Gasser
32. Functional Genomics Of Tick Vectors Challenged With The Cattle Parasite Babesia Bigemina
Ana Domingos, Sandra Antunes, Margarita Villar, and José de la Fuente33. Metagenomic Approaches To Disclose Disease-Associated Pathogens: Detection Of Viral Pathogens In Honeybees
Fredrik Granberg, Oskar E. Karlsson, and Sándor Belák
34. Proteomics Characterization Of Tick-Host-Pathogen Interactions
Marina Popara, Margarita Villar, and José de la Fuente
From the book reviews:
"This is an overview of molecular biology techniques for the detection of pathological states in animals. ... The audience includes doctors, laboratory technicians, researchers, and students of veterinary medicine and science. ... This is a nicely written, clinically relevant, cutting-edge book that gives a thorough overview of numerous molecular biology techniques for the detection of pathological diseases.” (Maureen McMichael, Doody's Book Reviews, March, 2015)
This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.
Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory.- Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice.- Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory.- Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes.- Biological Specimen Collection And Processing for Molecular Analysis.- Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory.- Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals.- Real Time Reverse Tranillegalscriptase PCR For The Detection Of Bluetongue Virus.- Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues.- A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants.- Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches.- Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique.- Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches.- Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection.- DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp. .- In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues.- Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections.- Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry .- Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases.- Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of AnimalPathogens.- Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms.- Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis.- Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci .- Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species.- Multilocus Sequence Typing (MLST): Markers For The Traceability Of Pathogenic Leptospira Strains.- Single Nucleotide Polymorphisms Discrimination Using High-Resolution Melting Analysis For The Genotyping Of Bacillus Anthracis.- Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Analysis Of Members Of The Mycobacterium Tuberculosis Complex.- Rapid Microarray-Based Genotyping Of Chlamydia Spp. Strains From Clinical Tissue Samples.- Multiplexed Genotyping Of Bacillus Anthracis By Luminex Xmap Suspension Array.- Next-Generation Sequencing In Veterinary Medicine: How Can The Massive Amount Of Information Arising From High-Throughput Technologies Improve Diagnosis, Control and Management Of Infectious Diseases?.- Impact Of Next-Generation Technologies On Exploring Socio-Economically Important Parasites And Developing New Interventions.- Functional Genomics Of Tick Vectors Challenged With The Cattle Parasite Babesia Bigemina.- Metagenomic Approaches To Disclose Disease-Associated Pathogens: Detection Of Viral Pathogens In Honeybees. - Proteomics Characterization Of Tick-Host-Pathogen Interactions.
From the book reviews:
"This is an overview of molecular biology techniques for the detection of pathological states in animals. ... The audience includes doctors, laboratory technicians, researchers, and students of veterinary medicine and science. ... This is a nicely written, clinically relevant, cutting-edge book that gives a thorough overview of numerous molecular biology techniques for the detection of pathological diseases." (Maureen McMichael, Doody's Book Reviews, March, 2015)
Inhaltsverzeichnis
Overview And Challenges Of Molecular Technologies In The Veterinary Microbiology Laboratory.- Significance And Integration Of Molecular Diagnostics in the Framework of Veterinary Practice.- Biosafety Principles And Practices for the Veterinary Diagnostic Laboratory.- Veterinary Biobank Facility: Development And Management For Diagnostic and Research Purposes.- Biological Specimen Collection And Processing for Molecular Analysis.- Validation Of Molecular Diagnostic Assays And Quality Assurance and Control in the Veterinary Laboratory.- Molecular Approaches To Recognize Relevant And Emerging Infectious Diseases In Animals.- Real Time Reverse Tranillegalscriptase PCR For The Detection Of Bluetongue Virus.- Nested And Multiplex Real Time PCR Using Dual-Labeled Probes: Detecting And Discriminating Mycobacterium Tuberculosis Complex Members In Cultures And Animal Tissues.- A Real-Time PCR Assay For The Diagnosis Of Gastrointestinal Nematode Infections of Small Ruminants.- Improved Detection Of Mycobacterium Bovis In Bovine Tissues Using Immunomagnetic Separation Approaches.- Detection Of Fish Pathogens By Loop-Mediated Isothermal Amplification (LAMP) Technique.- Direct Detection Of Theileria Annulata In Bovine Blood Samples Using Standard And Isothermal DNA Amplification Approaches.- Reverse Line Blot Hybridization With Species-Specific Oligonucleotide Probes: Application To Piroplasms Detection.- DNA Microarray-Based Detection Of Multiple Pathogens: Mycoplasma Spp. and Chlamydia Spp. .- In Situ Hybridization With Labeled Probes: Assessment of African Swine Fever Virus In Formalin-Fixed Paraffin-Embedded Tissues.- Fluorescence In Situ Hybridization For The Tissue Detection of Bacterial Pathogens Associated With Porcine Infections.- Identification Of Animal Pasteurellaceae By MALDI-TOF Mass Spectrometry .- Gold Nanoparticles as a Potential Tool For Diagnosis of Fish Diseases.- Nucleic Acid Testing, New Platforms And Nanotechnology For Point-of-Decision Diagnosis Of AnimalPathogens.- Molecular Typing Tools: From Pattern Recognition To Genome-Based Algorithms.- Analysis Of Campylobacter Jejuni And Campylobacter Coli Genotypes In Poultry Flocks By Restriction Fragment Length Polymorphism (RFLP) Analysis.- Pulsed-Field Gel Electrophoresis (PFGE): Application In Population Structure Studies Of Bovine Mastitis-Causing Streptococci .- Multiple-Locus Variable Number Tandem Repeat (VNTR) Analysis (MLVA) Using Multiplex PCR And Multicolour Capillary Electrophoresis: Application To The Genotyping Of Brucella Species.- Multilocus Sequence Typing (MLST): Markers For The Traceability Of Pathogenic Leptospira Strains.- Single Nucleotide Polymorphisms Discrimination Using High-Resolution Melting Analysis For The Genotyping Of Bacillus Anthracis.- Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Analysis Of Members Of The Mycobacterium Tuberculosis Complex.- Rapid Microarray-Based Genotyping Of Chlamydia Spp. Strains From Clinical Tissue Samples.- Multiplexed Genotyping Of Bacillus Anthracis By Luminex Xmap Suspension Array.- Next-Generation Sequencing In Veterinary Medicine: How Can The Massive Amount Of Information Arising From High-Throughput Technologies Improve Diagnosis, Control and Management Of Infectious Diseases?.- Impact Of Next-Generation Technologies On Exploring Socio-Economically Important Parasites And Developing New Interventions.- Functional Genomics Of Tick Vectors Challenged With The Cattle Parasite Babesia Bigemina.- Metagenomic Approaches To Disclose Disease-Associated Pathogens: Detection Of Viral Pathogens In Honeybees. - Proteomics Characterization Of Tick-Host-Pathogen Interactions.
Klappentext
This volume provides an overview of molecular biology tools for pathogen detection and characterization. Focusing on biobanking, biosafety, good laboratory practices, biological specimen collection and processing, quality assurance and control, validation of molecular diagnostic assays for veterinary use; molecular detection and identification of animal pathogens using a wide range of established techniques and covering, also, emerging diagnosis approaches; genotyping tools for assessing the genetic landscape and epidemiology of pathogens; and integrative omics and high-throughput technologies as powerful research tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.n nAuthoritative and practical, Veterinary Infection Biology: Molecular Diagnostics and High-Throughput Strategies aids scientists in continuing to study a broad range of molecular approaches, from simple and affordable to emerging and sophisticate.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
