Includes cutting-edge methods and protocols in mouse genetic research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols in mouse genetic research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.
Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.
Part I: Mouse Genetics Techniques
1. Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination
Masato Ohtsuka
2. Generation of Conditional Knockout Mice
Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy and Kay-Uwe Wagner
3. Generating Mouse Lines for Lineage Tracing and Knockout Studies
Petra Kraus, V. Sivakamasundari, Xing Xing, and Thomas Lufkin
4. Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)
Anton J.M. Roebroek and Bart Van Gool
5. Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection
Kun-Hsiung Lee
6. Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases
Susanne Feil, Jana Krauss, Martin Thunemann, and Robert Feil
7. Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice
Pedro N. Moreira and Lluís Montoliu
8. Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice
Jane Beil and Thorsten Buch
9. Generation of Genetically Engineered Mice by the piggyBac Transposon System
Sheng Ding, Tian Xu, and Xiaohui Wu
10. Generation and Applications of MADM-Based Mouse Genetic Mosaic System
Hui Zong
11. Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System
Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, and Vincenzo Coppola
12. In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis
Olga Simmons, Esther M. Bolanis, Jian Wang, and Simon J. Conway
Part II: Mouse Genetics in Stem Cells
13. Isolation and Handling of Mouse Embryonic Fibroblasts
Kanika Jain, Paul J. Verma, and Jun Liu
14. Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue
Pollyanna Agnes Goh and Paul J. Verma
15. Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells
Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, and Mugdha V. Joglekar
16. Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development
Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves, and Margaret H. Baron
17. Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System
Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han, and Yang Du
18. Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo
Lalit Sehgal, Abul Usmani, Sorab N. Dalal, and Subeer S. Majumdar
Part III: Mouse Genetics in Modeling Human Disease
19. Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts
Spencer W. Luebben, Naoko Shima, and Tsuyoshi Kawabata
20. Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment
Robert M. Hoffman
21. Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty
Viive M. Howell and Emily K. Colvin
22. Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans
Vishalakshi Chavali, Shyam Sundar Nandi, Shree Ram Singh, and Paras Kumar Mishra
23. Experimental Osteoarthritis Models in Mice
Julia Lorenz and Susanne Grässel
24. Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI)
Ganesan Ramesh and Punithavathi Ranganathan
25. Mouse Models of Acute and Chronic Colitis
Santhakumar Manicassamy and Indumathi Manoharan
26. Manipulation and Assessment of Gut Microbiome for Metabolic Studies
Sarang N. Satoor, Deepak P. Patil, Holly Kristensen, Mugdha V. Joglekar, Yogesh Shouche, and Anandwardhan A. Hardikar
Mouse Genetics: Methods and Protocols provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods, and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.
Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students, and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.
Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre-loxP Site-Specific Recombination.- Generation of Conditional Knockout Mice.- Generating Mouse Lines for Lineage Tracing and Knockout Studies.- Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE).- Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection.- Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases.- Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice.- Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice.- Generation of Genetically Engineered Mice by the piggyBac Transposon System.- Generation and Applications of MADM-Based Mouse Genetic Mosaic System.- Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System.- In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis.- Isolation and Handling of Mouse Embryonic Fibroblasts.- Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue.- Pdx1 (GFP/w) Mice for Isolation, Characterization and Differentiation of Pancreatic Progenitor Cells.- Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development.- Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System.- Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo.- Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts.- Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment.- Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty.- Generating Double Knockout Mice to Model Genetic Intervention for DiabeticCardiomyopathy in Humans.- Experimental Osteoarthritis Models in Mice.- Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI).- Mouse Models of Acute and Chronic Colitis.- Manipulation and Assessment of Gut Microbiome for Metabolic Studies.
Inhaltsverzeichnis
Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre-
loxP
Site-Specific Recombination.- Generation of Conditional Knockout Mice.- Generating Mouse Lines for Lineage Tracing and Knockout Studies.- Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE).- Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection.- Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases.- Intra cytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice.- Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice.- Generation of Genetically Engineered Mice by the
piggyBac
Transposon System.- Generation and Applications of MADM-Based Mouse Genetic Mosaic System.- Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System.- In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis.- Isolation and Handling of Mouse Embryonic Fibroblasts.- Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue.- Pdx1 (GFP/w) Mice for Isolation, Characterization and Differentiation of Pancreatic Progenitor Cells.- Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development.- Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System.- Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo.- Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts.- Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment.- Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty.- Generating Double Knockout Mice to Model Genetic Intervention for DiabeticCardiomyopathy in Humans.- Experimental Osteoarthritis Models in Mice.- Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI).- Mouse Models of Acute and Chronic Colitis.- Manipulation and Assessment of Gut Microbiome for Metabolic Studies.
Klappentext
Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.nComprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.
Includes cutting-edge methods and protocols in mouse genetic research
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
