Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
1. The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications
Natalie S. Poulter, William T.E. Pitkeathly, Philip J. Smith, and Joshua Z. Rappoport
2. Two-Photon Excitation Microscopy And Its Applications In Neuroscience
Ricardo Mostany, Amaya Miquelajauregui, Matthew Shtrahman, and Carlos Portera-Cailliau
3. Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy
Francesco Pampaloni, Roli Richa, Nariman Ansari, and Ernst H.K. Stelzer
4. Fluorescence Microscopy Based RNA Interference Screening
Manuel Gunkel, Nina Beil, Jürgen Beneke, Jürgen Reymann, and Holger Erfle
5. Fluorescence Resonance Energy Transfer Microscopy (FRET)
Kasia M. Kedziora and Kees Jalink
6. Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy
Yuansheng Sun, Vinod Jyothikumar, and Ammasi Periasamy
7. Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation
Bart Geverts, Martin E. van Royen, and Adriaan B. Houtsmuller
8. Fluorescence Correlation Spectroscopy
Mark A. Hink
9. Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules
Suvrajit Saha, Riya Raghupathy, and Satyajit Mayor
10. Practical Structured-Illumination Microscopy E. Hesper Rego and Lin Shao
11. 4Pi Microscopy of the Nuclear Pore Complex
Martin Kahms, Jana Hüve, and Reiner Peters
12. Application Of STED Microscopy To Cell Biology Questions
Natalia H. Revelo and Silvio O. Rizzoli
13. Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes
Kelsey Temprine, Andrew G. York, and Hari Shroff
14. Direct Stochastic Optical Reconstruction Microscopy (dSTORM)
Ulrike Endesfelder and Mike Heilemann
15. Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells
Taofei Yin and Yi I. Wu
This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications.- Two-Photon Excitation Microscopy And Its Applications In Neuroscience.- Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy.- Fluorescence Microscopy Based RNA Interference Screening.- Fluorescence Resonance Energy Transfer Microscopy (FRET).- Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy.- Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation.- Fluorescence Correlation Spectroscopy.- Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules.- Practical Structured-Illumination Microscopy.- 4Pi Microscopy of the Nuclear Pore Complex.- Application Of STED Microscopy To Cell Biology Questions.- Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes.- Direct Stochastic Optical Reconstruction Microscopy (dSTORM).- Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells.
Inhaltsverzeichnis
The Physical Basis Of Total Internal Reflection Fluorescence (TIRF) Microscopy and Its Cellular Applications.- Two-Photon Excitation Microscopy And Its Applications In Neuroscience.- Live Spheroid Formation Recorded with Light Sheet-based Fluorescence Microscopy
.-
Fluorescence Microscopy Based RNA Interference Screening.- Fluorescence Resonance Energy Transfer Microscopy (FRET).- Localizing Protein-Protein Interactions In Living Cells Using Fluorescence Lifetime Imaging Microscopy.- Analysis Of Biomolecular Dynamics By FRAP And Computer Simulation.- Fluorescence Correlation Spectroscopy.- Homo-FRET Imaging Highlights The Nanoscale Organization Of Cell Surface Molecules.- Practical Structured-Illumination Microscopy
.-
4Pi Microscopy of the Nuclear Pore Complex.- Application Of STED Microscopy To Cell Biology Questions.- Three-Dimensional Photoactivated Localization Microscopy With Genetically-Expressed Probes.-
Direct
Stochastic Optical Reconstruction Microscopy (
d
STORM).- Optogenetics: Optical Control Of A Photoactivatable Rac In Living Cells.
Klappentext
This volume provides an overview of advanced fluorescence microscopy, covering a broad range of methods. Each chapter focuses on a different method and provides a practical guide for application in biological systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.nAuthoritative and cutting-edge, Advanced Fluorescence Microscopy: Methods and Protocols seeks to provide scientists with methods for biological systems that are of interest.
Includes cutting-edge methods and protocols
n
Provides step-by-step detail essential for reproducible results
n
Contains key notes and implementation advice from the experts
n
Includes supplementary material: sn.pub/extras
n
