Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
This volume discusses a variety of animal models of diabetes, as well as describes techniques used to study end-points when using these models. The chapters in this book cover topics such as important considerations when working with mouse models of diabetes, highlighting factors that new investigators may not be aware of and some potential pitfalls in experimental outcomes; main characteristics of some commonly used animal models of diabetes research, ranging from mice to primates; animal models used to study specific aspects of beta-cell biology; and a focus on techniques used to assess blood glucose homeostasis, insulin action, and islet function in vivo and ex vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Animal Models of Diabetes: Methods and Protocols is a valuable resource that will help diabetes researchers design and carry out in vivo studies that will best suit their experimental questions and needs.
Preface...
Table of Contents...
Contributing Authors...
1.Practical Considerations when Using Mouse Models of Diabetes
Aileen J.F. King, Lydia F. Daniels Gatward, and Matilda R. Kennard
2.An Overview of Rodent Models of Obesity and Type 2 Diabetes
Thomas A. Lutz
3.Selectively Bred Diabetes Models: GK Rats, NSY Mice, and ON Mice
Mototsugu Nagao, Jonathan Lou S. Esguerra, Anna Wendt, Akira Asai, Hitoshi Sugihara, Shinichi Oikawa, and Lena Eliasson
4.A Review of Mouse Models of Monogenic Diabetes and ER Stress Signalling
Paraskevi Salpea, Cristina Cosentino, and Mariana Igoillo-Esteve
5.Rat Models of Human Type 1 Diabetes
Sigurd Lenzen, Tanja Arndt, Matthias Elsner, Dirk Wedekind, and Anne Jörns
6.Mouse Models of Autoimmune Diabetes: The Non-Obese Diabetic (NOD) Mouse
Dawei Chen, Terri C. Thayer, Li Wen, and F. Susan Wong
7.Mouse Models of Virus-Induced Type 1 Diabetes
Gustaf Christoffersson and Malin Flodström-Tullberg
8.Rat Models of Virus-Induced Type 1 Diabetes
James C. Needell and Danny Zipris
9.Large Animal Models of Diabetes
Barbara Ludwig, Eckhard Wolf, Uwe Schönmann, and Stefan Ludwig
10.Use of Streptozotocin in Rodent Models of Islet Transplantation
Aileen King, Elisabet Estil-les, and Eduard Montanya
11.Transplantation of Islets of Langerhans into the Anterior Chamber of the Eye for Longitudinal In Vivo Imaging
Christian M. Cohrs, Chunguang Chen, and Stephan Speier
12.Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish
Sumeet Pal Singh and Nikolay Ninoy
13.Generating Beta-Cell Specific Transgenic Mice Using the Cre-Lox System
Lorna I.F. Smith, Thomas G. Hill, and James E. Bowe
14.The Glucose Tolerance Test in Mice
Patricia Fonseca Pedro, Anastasia Tsakmaki, and Gavin A. Bewick
15.Assessment of Insulin Tolerance In Vivo in Mice
Irene Cózar-Castellano and Germán Perdomo
16.Continuous Glucose Monitoring in Conscious Unrestrained Mice
Aileen J.F. King, Matilda R. Kennard, and Manasi Nandi
17.Assessing Mouse Islet Function
Patricio Atanes, Inma Ruz-Maldonado, Oladapo E. Olaniru, and Shanta J. Persaud
18.Assessing Immune Responses in the Nonobese Diabetic Mouse Model of Type 1 Diabetes
Terri C. Thayer, Dimitri Kakabadse, Joanne Boldison, and F. Susan Wong
19.Assessment of Insulin Tolerance Ex Vivo
Irene Cózar-Castellano and Germán Perdomo
20.Using Pancreas Tissue Slices for the Study of Islet Physiology
Julia K. Panzer, Christian M. Cohrs, and Stephan Speier
21.Determining Beta Cell Mass, Apoptosis, Proliferation, and Individual Beta Cell Size in Pancreatic Sections
Noèlia Téllez and Eduard Montanya
Subject Index List...
This volume discusses a variety of animal models of diabetes, as well as describes techniques used to study end-points when using these models. The chapters in this book cover topics such as important considerations when working with mouse models of diabetes, highlighting factors that new investigators may not be aware of and some potential pitfalls in experimental outcomes; main characteristics of some commonly used animal models of diabetes research, ranging from mice to primates; animal models used to study specific aspects of beta-cell biology; and a focus on techniques used to assess blood glucose homeostasis, insulin action, and islet function in vivo and ex vivo. Written in the highly successful
Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive,
Animal Models of Diabetes: Methods and Protocols is a valuable resource that will help diabetes researchers design and carry out in vivo studies that will best suit their experimental questions and needs.
Practical Considerations when Using Mouse Models of Diabetes.- An Overview of Rodent Models of Obesity and Type 2 Diabetes.- Selectively Bred Diabetes Models: GK Rats, NSY Mice, and ON Mice.- A Review of Mouse Models of Monogenic Diabetes and ER Stress Signalling.- Rat Models of Human Type 1 Diabetes.- Mouse Models of Autoimmune Diabetes: The Non-Obese Diabetic (NOD) Mouse.- Mouse Models of Virus-Induced Type 1 Diabetes.- Rat Models of Virus-Induced Type 1 Diabetes.- Large Animal Models of Diabetes.- Use of Streptozotocin in Rodent Models of Islet Transplantation.- Transplantation of Islets of Langerhans into the Anterior Chamber of the Eye for Longitudinal In Vivo Imaging.- Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish.- Generating Beta-Cell Specific Transgenic Mice Using the Cre-Lox System.- The Glucose Tolerance Test in Mice.- Assessment of Insulin Tolerance In Vivo in Mice.- Continuous Glucose Monitoring in Conscious Unrestrained Mice.- Assessing Mouse Islet Function.- Assessing Immune Responses in the Nonobese Diabetic Mouse Model of Type 1 Diabetes.- Assessment of Insulin Tolerance Ex Vivo.- Using Pancreas Tissue Slices for the Study of Islet Physiology.- Determining Beta Cell Mass, Apoptosis, Proliferation, and Individual Beta Cell Size in Pancreatic Sections.
Inhaltsverzeichnis
Preface...
Table of Contents...
Contributing Authors...
1.Practical Considerations when Using Mouse Models of Diabetes
Aileen J.F. King, Lydia F. Daniels Gatward, and Matilda R. Kennard
2.An Overview of Rodent Models of Obesity and Type 2 Diabetes
Thomas A. Lutz
3.Selectively Bred Diabetes Models: GK Rats, NSY Mice, and ON Mice
Mototsugu Nagao, Jonathan Lou S. Esguerra, Anna Wendt, Akira Asai, Hitoshi Sugihara, Shinichi Oikawa, and Lena Eliasson
4.A Review of Mouse Models of Monogenic Diabetes and ER Stress Signalling
Paraskevi Salpea, Cristina Cosentino, and Mariana Igoillo-Esteve
5.Rat Models of Human Type 1 Diabetes
Sigurd Lenzen, Tanja Arndt, Matthias Elsner, Dirk Wedekind, and Anne Jörns
6.Mouse Models of Autoimmune Diabetes: The Non-Obese Diabetic (NOD) Mouse
Dawei Chen, Terri C. Thayer, Li Wen, and F. Susan Wong
7.Mouse Models of Virus-Induced Type 1 Diabetes
Gustaf Christoffersson and Malin Flodström-Tullberg
8.Rat Models of Virus-Induced Type 1 Diabetes
James C. Needell and Danny Zipris
9.Large Animal Models of Diabetes
Barbara Ludwig, Eckhard Wolf, Uwe Schönmann, and Stefan Ludwig
10.Use of Streptozotocin in Rodent Models of Islet Transplantation
Aileen King, Elisabet Estil-les, and Eduard Montanya
11.Transplantation of Islets of Langerhans into the Anterior Chamber of the Eye for Longitudinal In Vivo Imaging
Christian M. Cohrs, Chunguang Chen, and Stephan Speier
12.Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish
Sumeet Pal Singh and Nikolay Ninoy
13.Generating Beta-Cell Specific Transgenic Mice Using the Cre-Lox System
Lorna I.F. Smith, Thomas G. Hill, and James E. Bowe
14.The Glucose Tolerance Test in Mice
Patricia Fonseca Pedro, Anastasia Tsakmaki, and Gavin A. Bewick
15.Assessment of Insulin Tolerance In Vivo in Mice
Irene Cózar-Castellano and Germán Perdomo
16.Continuous Glucose Monitoring in Conscious Unrestrained Mice
Aileen J.F. King, Matilda R. Kennard, and Manasi Nandi
17.Assessing Mouse Islet Function
Patricio Atanes, Inma Ruz-Maldonado, Oladapo E. Olaniru, and Shanta J. Persaud
18.Assessing Immune Responses in the Nonobese Diabetic Mouse Model of Type 1 Diabetes
Terri C. Thayer, Dimitri Kakabadse, Joanne Boldison, and F. Susan Wong
19.Assessment of Insulin Tolerance Ex Vivo
Irene Cózar-Castellano and Germán Perdomo
20.Using Pancreas Tissue Slices for the Study of Islet Physiology
Julia K. Panzer, Christian M. Cohrs, and Stephan Speier
21.Determining Beta Cell Mass, Apoptosis, Proliferation, and Individual Beta Cell Size in Pancreatic Sections
Noèlia Téllez and Eduard Montanya
Subject Index List...
Klappentext
This volume discusses a variety of animal models of diabetes, as well as describes techniques used to study end-points when using these models. The chapters in this book cover topics such as important considerations when working with mouse models of diabetes, highlighting factors that new investigators may not be aware of and some potential pitfalls in experimental outcomes; main characteristics of some commonly used animal models of diabetes research, ranging from mice to primates; animal models used to study specific aspects of beta-cell biology; and a focus on techniques used to assess blood glucose homeostasis, insulin action, and islet function in vivo and ex vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
n
Cutting-edge and comprehensive, Animal Models of Diabetes: Methods and Protocols is a valuable resource that will help diabetes researchers design and carry out in vivo studies that will best suit their experimental questions and needs.
n
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
