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Advanced Fluorescence Reporters in Chemistry and Biology II
(Englisch)
Molecular Constructions, Polymers and Nanoparticles
Demchenko, Alexander P.

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Advanced Fluorescence Reporters in Chemistry and Biology II

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With contributions by numerous experts|A comprehensive review Written by experts Contains numerous color illustrations
General Aspects.- Nanocrystals and Nanoparticles Versus Molecular Fluorescent Labels as Reporters for Bioanalysis and the Life Sciences: A Critical Comparison.- Optimization of the Coupling of Target Recognition and Signal Generation.- Collective Effects Influencing Fluorescence Emission.- Encapsulated Dyes and Supramolecular Constructions.- Fluorescent J-Aggregates and Their Biological Applications.- Conjugates, Complexes, and Interlocked Systems Based on Squaraines and Cyanines.- Dye Doped Nanoparticles and Dendrimers.- Dye-Doped Polymeric Particles for Sensing and Imaging.- Silica-Based Nanoparticles: Design and Properties.- Luminescent Dendrimers as Ligands and Sensors of Metal Ions.- Prospects for Organic Dye Nanoparticles.- Luminescent Metal Nanoclusters.- Few-Atom Silver Clusters as Fluorescent Reporters.- Luminescent Quantum Clusters of Gold as Bio-Labels.- Conjugated Polymers.- Structure, Emissive Properties, and Reporting Abilities of Conjugated Polymers.- Optical Reporting by Conjugated Polymers via Conformational Changes.- Fluorescence Reporting Based on FRET Between Conjugated Polyelectrolyte and Organic Dye for Biosensor Applications.

From the reviews:

"This book is a comprehensive overview of the different types of nanoscale probes that have been and are currently being developed for sensing of analytes. The timely reference section allows the reader to locate key references to the primary literature for expansion of the material covered. ... a valuable resource that covers the synthetic, photo-physical, and theoretical aspects of this diverse field of research.” (Jeffrey T. Petty, Journal of the American Chemical Society, Vol. 133, 2011)

Avarietyof?uorescentandluminescentmaterialsintheformofmolecules,their complexes,andnanoparticlesareavailableforimplementationasreportingunits intosensingtechnologies. Increasingdemandsfromtheseapplicationareasrequire developmentofnew?uorescencereportersbasedonassociationandaggregationof ?uorescencedyesandontheirincorporationintodifferentnanostructures. Inter- tionsbetweenthesedyesandtheirincorporatingmatricesleadtonewspectroscopic effectsthatcanbeactivelyusedforoptimizingthesensordesign. Oneofthese effects is a spectacular formation of J-aggregates with distinct and very sharp excitationandemissionbands. Byincorporationintonanoparticles,organicdyes offer dramatically increased brightness together with improvement of chemical stabilityandphotostability. Moreover,certaindyescanformnanoparticlesth- selvessothattheirspectroscopicpropertiesareimproved. Semiconductorquantum dotsaretheothertypeofnanoparticles thatpossessuniqueandveryattractive photophysicalandspectroscopicproperties. Manyinterestingandnotfullyund- stoodphenomenaareobservedinclusterscomposedofonlyseveralatomsofnoble metals. Inconjugatedpolymers,strongelectronicconjugationbetweenelementary chromophoricunitsresultsindramaticeffectsinquenchingandinconformati- dependentspectroscopicbehavior. Possessingsuchpowerfulanddiversearsenaloftools,wehavetoexplorethem innovelsensingandimagingtechnologiesthatcombineincreasedbrightnessand sensitivityinanalytedetectionwithsimplicityandlowcostofproduction. The present book overviews the pathways for achieving this goal. In line with the discussion on monomeric ?uorescence reporters in the accompanying book (Vol. 8ofthisseries),aninsightfulanalysisofphotophysicalmechanismsbehind the ?uorescence response of composed and nanostructured materials is made. Based on the progress in understanding these mechanisms, their realization in differentchemicalstructuresisoverviewed. vii viii Preface Demonstratingtheprogressinaninterdisciplinary?eldofresearchanddev- opment,thisbookisprimarilyaddressedtospecialistswithdifferentbackground physicists, organic and analytical chemists, and photochemists to those who developandapplynew?uorescencereporters. Itwillalsobeusefultospecialists inbioanalysisandbiomedicaldiagnostics. Kyiv,Ukraine AlexanderP. Demchenko June2010 Contents PartI GeneralAspects NanocrystalsandNanoparticlesVersusMolecularFluorescent LabelsasReportersforBioanalysisandtheLifeSciences: ACriticalComparison . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 UteResch-Genger,MarkusGrabolle,RolandNitschke, andThomasNann OptimizationoftheCouplingofTargetRecognition andSignalGeneration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 AnaB. Descalzo,ShengchaoZhu,TobiasFischer,andKnutRurack CollectiveEffectsIn?uencingFluorescenceEmission . . . . . . . . . . . . . . . . . . . . . 107 AlexanderP. Demchenko PartII EncapsulatedDyesandSupramolecularConstructions FluorescentJ-AggregatesandTheirBiologicalApplications . . . . . . . . . . . . . 135 MykhayloYu. LosytskyyandValeriyM. Yashchuk Conjugates,Complexes,andInterlockedSystems BasedonSquarainesandCyanines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 LeonidD. Patsenker,AnatoliyL. Tatarets,OleksiiP. Klochko, andEwaldA. Terpetschnig PartIII Dye-DopedNanoparticlesandDendrimers Dye-DopedPolymericParticlesforSensingandImaging . . . . . . . . . . . . . . . . 193 SergeyM. Borisov,TorstenMayr,Gu nterMistlberger,andIngoKlimant ix x Contents Silica-BasedNanoparticles:DesignandProperties . . . . . . . . . . . . . . . . . . . . . . . 229 SongLiang,CarrieL. John,ShupingXu,JiaoChen,YuhuiJin, QuanYuan,WeihongTan,andJuliaX. Zhao LuminescentDendrimersasLigandsandSensors ofMetalIons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253 GiacomoBergamini,EnricoMarchi,and
General aspects.- Fluorescence reporters in sensing technologies.- Cell imaging with organic dyes.- Cell imaging with nanoscale devices.- Cell imaging with visible fluorescent proteins.- Tissue and whole body imaging.

From the reviews:

"This book is a comprehensive overview of the different types of nanoscale probes that have been and are currently being developed for sensing of analytes. The timely reference section allows the reader to locate key references to the primary literature for expansion of the material covered. ... a valuable resource that covers the synthetic, photo-physical, and theoretical aspects of this diverse field of research." (Jeffrey T. Petty, Journal of the American Chemical Society, Vol. 133, 2011)

Inhaltsverzeichnis



General Aspects.- Nanocrystals and Nanoparticles Versus Molecular Fluorescent Labels as Reporters for Bioanalysis and the Life Sciences: A Critical Comparison.- Optimization of the Coupling of Target Recognition and Signal Generation.- Collective Effects Influencing Fluorescence Emission.- Encapsulated Dyes and Supramolecular Constructions.- Fluorescent J-Aggregates and Their Biological Applications.- Conjugates, Complexes, and Interlocked Systems Based on Squaraines and Cyanines.- Dye Doped Nanoparticles and Dendrimers.- Dye-Doped Polymeric Particles for Sensing and Imaging.- Silica-Based Nanoparticles: Design and Properties.- Luminescent Dendrimers as Ligands and Sensors of Metal Ions.- Prospects for Organic Dye Nanoparticles.- Luminescent Metal Nanoclusters.- Few-Atom Silver Clusters as Fluorescent Reporters.- Luminescent Quantum Clusters of Gold as Bio-Labels.- Conjugated Polymers.- Structure, Emissive Properties, and Reporting Abilities of Conjugated Polymers.- Optical Reporting by Conjugated Polymers via Conformational Changes.- Fluorescence Reporting Based on FRET Between Conjugated Polyelectrolyte and Organic Dye for Biosensor Applications.


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With contributions by numerous experts


A comprehensive review Written by experts Contains numerous color illustrations

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Includes supplementary material: sn.pub/extras

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