Offers cutting-edge methods for cancer gene profiling
Includes methods for gene expression profiling and array CGH, as well as protein analysis
Provides information about collecting clinical samples and their use in microdissection
Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research.
Cancer Gene Profiling: Methods and ProtocolsRobert Grützmann and Christian Pilarsky, eds. Table of ContentsPrefaceContributors1. Organizational Issues in Providing High Quality Human Tissues and Clinical Information for the Support of Biomedical ResearchWalter C. Bell, Katherine C. Sexton and William E. Grizzle2. Manual microdissectionGlen Kristiansen3. Laser MicrodissectionAnja Rabien4. Tissue MicroarraysAna-Maria Dancau, Ronald Simon, Martina Mirlacher and Guido Sauter5. A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular FunctionHenri Sara, Olli Kallioniemi and Matthias Nees6. Mining EST libraries for cancer associated genesArmin Schmitt7. Automated fluorescent differential display (FDD) for cancer gene profilingJonathan D. Meade, Yong-jig Cho, Blake R. Shester, Jamie C. Walden, Zhen Guo and Peng Liang8. Manual microdissection combined with aRNA-longSAGE for the analysis of limited cell numbersJutta Lüttges, Stephan A. Hahn and Anna M. Heidenblut9. Quantitative DNA Methylation Profiling on a High Density Oligonucleotide MicroarrayAnne Fassbender, Jörn Lewin, Thomas König, Tamas Rujan, Cecile Pelet, Ralf Lesche, Jürgen Distler and Matthias Schuster10. SNP analysis to associate cancer riskJulie Earl and William Greenhalf11. Application of proteomics in cancer gene profiling: 2D-DIGEDeepak Hariharan, Mark E Weeks and Tatjana Crnogorac-Jurcevic12. Search for and Identification of Novel Tumor associated AutoantigensKarsten Conrad, Holger Bartsch, Ulrich Canzler, Christian Pilarsky, Robert Grützmann and Michael Bachmann13. Integrative Oncogenomic Analysis OfMicroarray Data In Hematologic MalignanciesJose A Martínez-Climent, Lorena Fontan, Vicente Fresquet, Eloy Robles, María Ortiz and Angel Rubio14. Cancer gene profiling in pancreatic cancerFelip Vilardell Villellas and Christine A. Iacobuzio-Donahue15. Cancer Gene Profiling in Prostate CancerAdam Foye and Phillip G. Febbo16. Cancer Gene Profiling for Response PredictionB. Michael Ghadimi and Marian Grade17. The EGFR pathway as an example for genotype – phenotype correlation in tumor genesUlrike Mogck, Eray Goekkurt and Jan Stoehlmacher18. Quantitation of CD39 gene expression in pancreatic tissue by real-time polymerase chain reaction (qRT-PCR)Martin Loos, Beat Künzli, and Helmut Friess19: Functional profiling methods in cancerJoaquín Dopazo20: Calibration of microarray gene-expression dataHans Binder, Stephan Preibisch and Hilmar Berger21. Meta-analysis of Cancer Gene Profiling DataXinan Yang and Xiao Sun22. Target gene discovery for novel therapeutic agents in cancer treatmentOle Ammerpohl, Sanjay Tiwari and Holger Kalthoff
From the reviews:
"The team of over 60 contributors put together an impressive compilation, covering a whole process of genetic analysis of cancer tissue ... . Usefulness of the book is increased by the fact that most of the methods are optimized for real-life clinical tissue samples not just cell lines. ... All in all, this book clearly demonstrated that the molecular methods used in the diagnosis and research of cancer, and of any other disease for that matter, entered the 21st century.” (J. Markus, Neoplasma, March, 2011)
Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research.
Organizational Issues in Providing High-Quality Human Tissues and Clinical Information for the Support of Biomedical Research.- Manual Microdissection.- Laser Microdissection.- Tissue Microarrays.- A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function.- Mining Expressed Sequence Tag (EST) Libraries for Cancer-Associated Genes.- Automated Fluorescent Differential Display for Cancer Gene Profiling.- Manual Microdissection Combined with Antisense RNA-LongSAGE for the Analysis of Limited Cell Numbers.- Quantitative DNA Methylation Profiling on a High-Density Oligonucleotide Microarray.- Single-Nucleotide Polymorphism (SNP) Analysis to Associate Cancer Risk.- Application of Proteomics in Cancer Gene Profiling: Two-Dimensional Difference in Gel Electrophoresis (2D-DIGE).- Search for and Identification of Novel Tumor-Associated Autoantigens.- Integrative Oncogenomic Analysis of Microarray Data in Hematologic Malignancies.- Cancer Gene Profiling in Pancreatic Cancer.- Cancer Gene Profiling in Prostate Cancer.- Cancer Gene Profiling for Response Prediction.- The EGFR Pathway as an Example for Genotype: Phenotype Correlation in Tumor Genes.- Quantitation of CD39 Gene Expression in Pancreatic Tissue by Real-Time Polymerase Chain Reaction.- Functional Profiling Methods in Cancer.- Calibration of Microarray Gene-Expression Data.- Meta-analysis of Cancer Gene-Profiling Data.- Target Gene Discovery for Novel Therapeutic Agents in Cancer Treatment.
From the reviews:
"The team of over 60 contributors put together an impressive compilation, covering a whole process of genetic analysis of cancer tissue ... . Usefulness of the book is increased by the fact that most of the methods are optimized for real-life clinical tissue samples not just cell lines. ... All in all, this book clearly demonstrated that the molecular methods used in the diagnosis and research of cancer, and of any other disease for that matter, entered the 21st century." (J. Markus, Neoplasma, March, 2011)
Inhaltsverzeichnis
Cancer Gene Profiling: Methods and Protocols
Robert Grützmann and Christian Pilarsky, eds.
Table of Contents
Preface
Contributors
1. Organizational Issues in Providing High Quality Human Tissues and Clinical Information for the Support of Biomedical Research
Walter C. Bell, Katherine C. Sexton and William E. Grizzle
2. Manual microdissection
Glen Kristiansen
3. Laser Microdissection
Anja Rabien
4. Tissue Microarrays
Ana-Maria Dancau, Ronald Simon, Martina Mirlacher and Guido Sauter
5. A Decade of Cancer Gene Profiling: From Molecular Portraits to Molecular Function
Henri Sara, Olli Kallioniemi and Matthias Nees
6. Mining EST libraries for cancer associated genes
Armin Schmitt
7. Automated fluorescent differential display (FDD) for cancer gene profiling
Jonathan D. Meade, Yong-jig Cho, Blake R. Shester, Jamie C. Walden, Zhen Guo and Peng Liang
8. Manual microdissection combined with aRNA-longSAGE for the analysis of limited cell numbers
Jutta Lüttges, Stephan A. Hahn and Anna M. Heidenblut
9. Quantitative DNA Methylation Profiling on a High Density Oligonucleotide Microarray
Anne Fassbender, Jörn Lewin, Thomas König, Tamas Rujan, Cecile Pelet, Ralf Lesche, Jürgen Distler and Matthias Schuster
10. SNP analysis to associate cancer risk
Julie Earl and William Greenhalf
11. Application of proteomics in cancer gene profiling: 2D-DIGE
Deepak Hariharan, Mark E Weeks and Tatjana Crnogorac-Jurcevic
12. Search for and Identification of Novel Tumor associated Autoantigens
Karsten Conrad, Holger Bartsch, Ulrich Canzler, Christian Pilarsky, Robert Grützmann and Michael Bachmann
13. Integrative Oncogenomic Analysis OfMicroarray Data In Hematologic Malignancies
Jose A Martínez-Climent, Lorena Fontan, Vicente Fresquet, Eloy Robles, María Ortiz and Angel Rubio
14. Cancer gene profiling in pancreatic cancer
Felip Vilardell Villellas and Christine A. Iacobuzio-Donahue
15. Cancer Gene Profiling in Prostate Cancer
Adam Foye and Phillip G. Febbo
16. Cancer Gene Profiling for Response Prediction
B. Michael Ghadimi and Marian Grade
17. The EGFR pathway as an example for genotype - phenotype correlation in tumor genes
Ulrike Mogck, Eray Goekkurt and Jan Stoehlmacher
18. Quantitation of CD39 gene expression in pancreatic tissue by real-time polymerase chain reaction (qRT-PCR)
Martin Loos, Beat Künzli, and Helmut Friess
19: Functional profiling methods in cancer
Joaquín Dopazo
20: Calibration of microarray gene-expression data
Hans Binder, Stephan Preibisch and Hilmar Berger
21. Meta-analysis of Cancer Gene Profiling Data
Xinan Yang and Xiao Sun
22. Target gene discovery for novel therapeutic agents in cancer treatment
Ole Ammerpohl, Sanjay Tiwari and Holger Kalthoff
Klappentext
Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.nnComprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research.
Offers cutting-edge methods for cancer gene profiling
Includes methods for gene expression profiling and array CGH, as well as protein analysis
Provides information about collecting clinical samples and their use in microdissection
