Introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models
Provides step-by-step detail essential for reproducible results
Contains key notes and expert implementation advice
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge,
Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Part I: Drosophila Protocols
1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain
Danielle C. Diaper and Frank Hirth
2. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila
David Jussen and Rolf Urbach
3. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling
Karoline F. Kraft and Rolf Urbach
4. Flybow to Dissect Circuit Assembly in the Drosophila Brain
Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker
5. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe
Benjamin Perruchoud and Boris Egger
6. Using MARCM to Study Drosophila Brain Development
Gudrun Viktorin
Part II: Other Arthropods
7. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria
George Boyan and Yu Liu
Part III: Molluscs
8. Methods in Brain Development of Molluscs
Andreas Wanninger and Tim Wollesen
Part IV: Xenopus Protocols
9. In Situ Hybridization and Immunostaining of Xenopus Brain
Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu
10. Microinjection Manipulations in the Elucidation of Xenopus Brain Development
Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford
11. Morpholino Studies in Xenopus Brain Development
Jennifer E. Bestman and Hollis T. Cline
Part V: Zebrafish Protocols
12. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification
Gilbert Lauter, Iris Söll, and Giselbert Hauptmann
13. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish
Adèle Faucherre and Hernán López-Schier
14. Anatomical Dissection of Zebrafish Brain Development
Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins
Part VI: Chicken Protocols
15. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain
Richard P. Tucker and Qizhi Gong
16. Transplantation of Neural Tissue: Quail-Chick Chimeras
Andrea Streit and Claudio D. Stern
17. RNAi-Based Gene Silencing in Chicken Brain Development
Irwin Andermatt and Esther T. Stoeckli
Part VII: Mouse Protocols
18. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development
Jinling Liu and Aimin Liu
19. In Utero Electroporation to Study Mouse Brain Development
Emilie Pacary and François Guillemot
20. The Cre/Lox System to Assess the Development of the Mouse Brain
Claudius F. Kratochwil and Filippo M. Rijli
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge,
Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Immunostaining of the Developing Embryonic and Larval Drosophila Brain.- Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila.- Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling.- Flybow to Dissect Circuit Assembly in the Drosophila Brain.- Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe.- Using MARCM to Study Drosophila Brain Development.- Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria.- Methods in Brain Development of Molluscs.- In Situ Hybridization and Immunostaining of Xenopus Brain.- Microinjection Manipulations in the Elucidation of Xenopus Brain Development.- Morpholino Studies in Xenopus Brain Development.- Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification.- Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish.- Anatomical Dissection of Zebrafish Brain Development.- Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain.- Transplantation of Neural Tissue: Quail-Chick Chimeras.- RNAi-Based Gene Silencing in Chicken Brain Development.- Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development.- In Utero Electroporation to Study Mouse Brain Development.- The Cre/Lox System to Assess the Development of the Mouse Brain.
Inhaltsverzeichnis
Part I: Drosophila Protocols
1. Immunostaining of the Developing Embryonic and Larval Drosophila Brain
Danielle C. Diaper and Frank Hirth
2. Non-Fluorescent RNA In Situ-Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila
David Jussen and Rolf Urbach
3. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI-Labeling
Karoline F. Kraft and Rolf Urbach
4. Flybow to Dissect Circuit Assembly in the Drosophila Brain
Nana Shimosako, Dafni Hadjieconomou, and Iris Salecker
5. Immunofluorescent Labeling of Neural Stem Cells in the Drosophila Optic Lobe
Benjamin Perruchoud and Boris Egger
6. Using MARCM to Study Drosophila Brain Development
Gudrun Viktorin
Part II: Other Arthropods
7. Dye Coupling and Immunostaining of Astrocyte-Like Glia Following Intracellular Injection of Fluorochromes in Brain Slices of the Grasshopper, Schistocerca gregaria
George Boyan and Yu Liu
Part III: Molluscs
8. Methods in Brain Development of Molluscs
Andreas Wanninger and Tim Wollesen
Part IV: Xenopus Protocols
9. In Situ Hybridization and Immunostaining of Xenopus Brain
Kai-li Liu, Xiu-mei Wang, Zi-long Li, and Ying Liu
10. Microinjection Manipulations in the Elucidation of Xenopus Brain Development
Cristine Smoczer, Lara Hooker, Saqib S. Sachani, and Michael J. Crawford
11. Morpholino Studies in Xenopus Brain Development
Jennifer E. Bestman and Hollis T. Cline
Part V: Zebrafish Protocols
12. Sensitive Whole-Mount Fluorescent In Situ Hybridization in Zebrafish Using Enhanced Tyramide Signal Amplification
Gilbert Lauter, Iris Söll, and Giselbert Hauptmann
13. Dynamic Neuroanatomy at Subcellular Resolution in the Zebrafish
Adèle Faucherre and Hernán López-Schier
14. Anatomical Dissection of Zebrafish Brain Development
Katherine J. Turner, Thomas G. Bracewell, and Thomas A. Hawkins
Part VI: Chicken Protocols
15. Immunohistochemistry and In Situ Hybridization in the Developing Chicken Brain
Richard P. Tucker and Qizhi Gong
16. Transplantation of Neural Tissue: Quail-Chick Chimeras
Andrea Streit and Claudio D. Stern
17. RNAi-Based Gene Silencing in Chicken Brain Development
Irwin Andermatt and Esther T. Stoeckli
Part VII: Mouse Protocols
18. Immunohistochemistry and RNA In Situ Hybridization in Mouse Brain Development
Jinling Liu and Aimin Liu
19. In Utero Electroporation to Study Mouse Brain Development
Emilie Pacary and François Guillemot
20. The Cre/Lox System to Assess the Development of the Mouse Brain
Claudius F. Kratochwil and Filippo M. Rijli
Klappentext
Providing widely used techniques in genetic model systems and many complementing animal models, Brain Development: Methods and Protocols focuses its expert contributions on two key technical aspects of developmental neurobiology: detection of gene expression and functional characterization of developmental control genes. Covering animal models such as the fruit fly, zebra fish, chicken, and mouse, this detailed book views in situ hybridization, reporter gene expression, and immunohistochemical staining methods, as well as RNA interference, Morpholino, or transgenic techniques through the prism of these models. Written in the highly successful Methods in Molecular Biology series format, chapter include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.n Practical and cutting-edge, Brain Development: Methods and Protocols aims to provide precise technical protocols but also allows for comparing a wide range of protocols in different tissues and species.
Introduces cutting-edge techniques in developmental neurobiology through genetic model systems as well as some complimentary animal models
Provides step-by-step detail essential for reproducible results
Contains key notes and expert implementation advice
