Includes key methods and protocols aimed at exploring the imaging and tracking of a wide variety of stem cells
Provides step-by-step detail essential for reproducible results
Contains expert notes and key implementation advice
Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field. As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Imaging and Tracking Stem Cells: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in tracking stem cells and their progeny.
1. Primary Culture and Live-Imaging of Adult Neural Stem Cells and Their Progeny
Felipe Ortega, Benedikt Berninger, and Marcos R. Costa
2. Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology
Marie-Therese Armentero, Patrizia Bossolasco, and Lidia Cova
3. High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes
Gábor Földes and Maxime Mioulane
4. A High Content Imaging-Based Approach for Classifying Cellular Phenotypes
Joseph J. Kim, Sebastián L. Vega, and Prabhas V. Moghe
5. Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions
Go Nagamatsu and Toshio Suda
6. Imaging and Tracking of Bone-Marrow-Derived Immune and Stem Cells
Youbo Zhao, Andrew J. Bower, Benedikt W. Graf, Marni D. Boppart, and Stephen A. Boppart
7. Co-Visualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3-D DNA Methylation Phenotyping of Stem Cells
Jian Tajbakhsh
8. Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention
HuaLei Zhang and Rong Zhou
9. Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice
Takaya Abe, Shinichi Aizawa, and Toshihiko Fujimori
10. Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo
Minjung Kang, Panagiotis Xenopoulos, Silvia Muñoz-Descalzo, Xinghua Lou, and Anna-Katerina Hadjantonakis
11. Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Post-Mortem Multi-Color Histological Analysis
Kristien Reekmans, Nathalie De Vocht, Jelle Praet, Debbie Le Blon, Chloé Hoornaert, Jasmijn Daans, Annemie Van der Linden, Zwi Berneman, and Peter Ponsaerts
12. Micro-CT Technique for Three Dimensional Visualization of Human Stem Cells
Andrea Farini, ChiaraVilla, Marzia Belicchi, Mirella Meregalli, and Yvan Torrente
13. Non-Invasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging
Annette Tennstaedt, Markus Aswendt, Joanna Adamczak, and Mathias Hoehn
14. Magnetic Resonance Imaging and Tracking of Stem Cells
Hossein Nejadnik, Rostislav Castillo, and Heike E. Daldrup-Link
15. Whole Body MRI Followed by Fluorescent Microscopy Analysis for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intracardiac Injection, Skeletal Muscle Transplantation, or Systemic Delivery
Boris Odintsov, Ju Lan Chun, and Suzanne E. Berry
16. Molecular Imaging and Tracking Stem Cells in Neurosciences
Toma Spiriev, Nora Sandu, and Bernhard Schaller
17. Bioluminescence Imaging of Human Embryonic Stem Cells-Derived Endothelial Cells for Treatment of Myocardial Infarction
Weijun Su, Liang Leng, Zhongchao Han, Zuoxiang He, and Zongjin Li
Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro.
This book presents key methods and protocols aimed at exploring the imaging and tracking of a wide variety of stem cells. It provides step-by-step detail essential for reproducible results as well as offers key implementation advice.
Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field. As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Imaging and Tracking Stem Cells: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in tracking stem cells and their progeny.
Primary Culture and Live-Imaging of Adult Neural Stem Cells and Their Progeny.- Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology.- High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes.- A High Content Imaging-Based Approach for Classifying Cellular Phenotypes.- Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions.- Imaging and Tracking of Bone-Marrow-Derived Immune and Stem Cells.- Co-Visualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3-D DNA Methylation Phenotyping of Stem Cells.- Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention.- Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice.- Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo.- Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Post-Mortem Multi-Color Histological Analysis.- Micro-CT Technique for Three Dimensional Visualization of Human Stem Cells.- Non-Invasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging.- Magnetic Resonance Imaging and Tracking of Stem Cells.- Whole Body MRI Followed by Fluorescent Microscopy Analysis for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intracardiac Injection, Skeletal Muscle Transplantation, or Systemic Delivery.- Molecular Imaging and Tracking Stem Cells in Neurosciences.- Bioluminescence Imaging of Human Embryonic Stem Cells-Derived Endothelial Cells for Treatment of Myocardial Infarction.
Inhaltsverzeichnis
Primary Culture and Live-Imaging of Adult Neural Stem Cells and Their Progeny.- Labeling and Tracking of Human Mesenchymal Stem Cells Using Near-Infrared Technology.- High Content Imaging and Analysis of Pluripotent Stem Cell-Derived Cardiomyocytes.- A High Content Imaging-Based Approach for Classifying Cellular Phenotypes.- Conversion of Primordial Germ Cells to Pluripotent Stem Cells: Methods for Cell Tracking and Culture Conditions.- Imaging and Tracking of Bone-Marrow-Derived Immune and Stem Cells.- Co-Visualization of Methylcytosine, Global DNA, and Protein Biomarkers for In Situ 3-D DNA Methylation Phenotyping of Stem Cells.- Noninvasive Imaging of Myocardial Blood Flow Recovery in Response to Stem Cell Intervention.- Live Imaging of Early Mouse Embryos Using Fluorescently Labeled Transgenic Mice.- Live Imaging, Identifying, and Tracking Single Cells in Complex Populations In Vivo and Ex Vivo.- Quantitative Evaluation of Stem Cell Grafting in the Central Nervous System of Mice by In Vivo Bioluminescence Imaging and Post-Mortem Multi-Color Histological Analysis.- Micro-CT Technique for Three Dimensional Visualization of Human Stem Cells.- Non-Invasive Multimodal Imaging of Stem Cell Transplants in the Brain Using Bioluminescence Imaging and Magnetic Resonance Imaging.- Magnetic Resonance Imaging and Tracking of Stem Cells.- Whole Body MRI Followed by Fluorescent Microscopy Analysis for Detection of Stem Cells Labeled with Superparamagnetic Iron Oxide (SPIO) Nanoparticles and DiI Following Intracardiac Injection, Skeletal Muscle Transplantation, or Systemic Delivery.- Molecular Imaging and Tracking Stem Cells in Neurosciences.- Bioluminescence Imaging of Human Embryonic Stem Cells-Derived Endothelial Cells for Treatment of Myocardial Infarction.
Klappentext
Imaging and Tracking Stem Cells: Methods and Protocols gathers representative protocols related to the vital techniques of stem cell imaging and lineage tracing, including that of live cells, both in vivo and in vitro. The detailed chapters presented within have been validated for reproducibility and are described in an easy to follow, step-by-step fashion so as to be valuable for not only experts but also novices in the stem cell field. As with other volumes in the highly successful Methods in Molecular Biology series, chapters conclude with a Notes section, which provides tips on troubleshooting and avoiding known pitfalls.n nAuthoritative and practical, Imaging and Tracking Stem Cells: Methods and Protocols provides both a flavor of the field as it currently is and a source to stimulate new approaches and methodologies by those interested in tracking stem cells and their progeny.
Includes key methods and protocols aimed at exploring the imaging and tracking of a wide variety of stem cells
Provides step-by-step detail essential for reproducible results
Contains expert notes and key implementation advice
Includes supplementary material: sn.pub/extras
