Sets the stage for future research and laboratory application involving embryo culture
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.
1. Historical Background of Gamete and Embryo Culture
Jacques Cohen and Don Rieger
2. Utility of Animal Models for Human Embryo Culture Development – Rodents
Gary D. Smith
3. Utility of Animal Models for Human Embryo Culture Development—Domestic Species
Rebecca L. Krisher
4. Utility of Animal Models for Human Embryo Culture: Non-Human Primates
Calvin Simerly and Gerald Schatten
5. Media Composition: Salts and Osmolality
Jay M. Baltz
6. Media Composition: Energy Sources and Metabolism
Deirdre Zander-Fox and Michelle Lane
7. Media Composition – Amino Acids and Cellular Homeostasis
Franchesca D. Houghton
8. Media Composition: Macromolecules and Embryo Growth
Marius Meintjes
9. Media Composition- Antioxidants/Chelators and Cellular Function
Catherine M.H. Combelles and Margo L. Hennet
10. Media Composition: pH and Buffers
Jason E. Swain
11. Media Composition, Growth Factors
Aparna Hegde
12. Culture Systems – Single Step
Ronit Machtinger, Catherine Racowsky
13. Culture Systems - Sequential
Patrick Quinn
14. Embryo Coculture
Yves J R Ménézo , Edouard Servy, Anna Veiga, André Hazout , Kay Elder
15. Low Oxygen Culture
Borut Kovacic
16. Culture Methods – Embryo density
Michael L. Reed
17. Culture Medium - Air Quality
Theodore Thomas
18. Mineral Oil Overlay and Embryo Culture
Dean E. Morbeck and Phoebe M. Hughes
19. Physiological and Environmental Factors That Can Affect the Outcome of Human ART
Don Rieger
20. Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)
André Monteiro da Rocha and Gary D. Smith
21. Human Embryo Culture Media Comparisons
Thomas B. Pool, John Schoolfield and David Han
22. Embryo Culture and Monozygotic Twinning
Amy E. Sparks
23. Embryo Culture and Epigenetics
Brenna A. Market Velker, Michelle M. Denomme and Mellissa R.W. Mann
André Monteiro da Rocha and Gary D. Smith
21. Human Embryo Culture Media Comparisons
Thomas B. Pool, John Schoolfield and David Han
22. Embryo Culture and Monozygotic Twinning
Amy E. Sparks
23. Embryo Culture and Epigenetics
Brenna A. Market Velker, Michelle M. Denomme and Mellissa R.W. Mann
André Monteiro da Rocha and Gary D. Smith
21. Human Embryo Culture Media Comparisons
Thomas B. Pool, John Schoolfield and David Han
22. Embryo Culture and Monozygotic Twinning
Amy E. Sparks
23. Embryo Culture and Epigenetics
Brenna A. Market Velker, Michelle M. Denomme and Mellissa R.W. Mann
From the reviews:
"It has been a really pleasure to follow the presentation of all the topics related to the improvements occurring in human embryo culture as they are here presented. ... The editors, and all of the authors, have to be acknowledged for this very well done book that will help researchers, clinicians and reproductive biologists ... .” (CarloAlberto Redi, European Journal of Histochemistry, Vol. 57, 2013)
This Methods in Molecular Biology(TM) book covers the complete range of contemporary methods for the study of human embryo culture. Includes lists of necessary materials and reagents, step-by-step laboratory protocols, and key tips on troubleshooting and pitfalls.
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.
Historical Background of Gamete and Embryo Culture.-Utility of Animal Models for Human Embryo Culture Development - Rodents.-Utility of Animal Models for Human Embryo Culture Development-Domestic Species.-Utility of Animal Models for Human Embryo Culture: Non-Human Primates.-Media Composition: Salts and Osmolality.-Media Composition: Energy Sources and Metabolism.-Media Composition - Amino Acids and Cellular Homeostasis.-Media Composition: Macromolecules and Embryo Growth.-Media Composition- Antioxidants/Chelators and Cellular Function.-Media Composition: pH and Buffers.-Media Composition, Growth Factors.-Culture Systems - Single Step.-Culture Systems - Sequential.-Embryo Coculture.-Low Oxygen Culture.-Culture Methods - Embryo Density.-Culture Medium - Air Quality.-Mineral Oil Overlay and Embryo Culture.-Physiological and Environmental Factors That Can Affect the Outcome of Human ART.-Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics.-Human Embryo Culture Media Comparisons.-Embryo Culture and Monozygotic Twinning.-Embryo Culture and Epigenetics.
From the reviews:
"It has been a really pleasure to follow the presentation of all the topics related to the improvements occurring in human embryo culture as they are here presented. ... The editors, and all of the authors, have to be acknowledged for this very well done book that will help researchers, clinicians and reproductive biologists ... ." (CarloAlberto Redi, European Journal of Histochemistry, Vol. 57, 2013)
Inhaltsverzeichnis
1. Historical Background of Gamete and Embryo CultureJacques Cohen and Don Rieger 2. Utility of Animal Models for Human Embryo Culture Development ¿ RodentsGary D. Smith 3. Utility of Animal Models for Human Embryo Culture Development¿Domestic SpeciesRebecca L. Krisher4. Utility of Animal Models for Human Embryo Culture: Non-Human PrimatesCalvin Simerly and Gerald Schatten5. Media Composition: Salts and OsmolalityJay M. Baltz6. Media Composition: Energy Sources and MetabolismDeirdre Zander-Fox and Michelle Lane7. Media Composition ¿ Amino Acids and Cellular HomeostasisFranchesca D. Houghton 8. Media Composition: Macromolecules and Embryo GrowthMarius Meintjes 9. Media Composition- Antioxidants/Chelators and Cellular FunctionCatherine M.H. Combelles and Margo L. Hennet10. Media Composition: pH and Buffers Jason E. Swain 11. Media Composition, Growth FactorsAparna Hegde 12. Culture Systems ¿ Single StepRonit Machtinger, Catherine Racowsky13. Culture Systems - Sequential Patrick Quinn 14. Embryo CocultureYves J R Ménézo , Edouard Servy, Anna Veiga, André Hazout , Kay Elder 15. Low Oxygen CultureBorut Koväi¿16. Culture Methods ¿ Embryo densityMichael L. Reed 17. Culture Medium - Air Quality Theodore Thomas 18. Mineral Oil Overlay and Embryo CultureDean E. Morbeck and Phoebe M. Hughes 19. Physiological and Environmental Factors That Can Affect the Outcome of Human ARTDon Rieger 20. Culture Systems: Fluid Dynamic Embryo Culture Systems (Microfluidics)André Monteiro da Rocha and Gary D. Smith 21. Human Embryo Culture Media ComparisonsThomas B. Pool, John Schoolfield and David Han 22. Embryo Culture and Monozygotic TwinningAmy E. Sparks 23. Embryo Culture and EpigeneticsBrenna A. Market Velker, Michelle M. Denomme and Mellissa R.W. Mann André Monteiro da Rocha and Gary D. Smith 21. Human Embryo Culture Media ComparisonsThomas B. Pool, John Schoolfield and David Han 22. Embryo Culture and Monozygotic TwinningAmy E. Sparks 23. Embryo Culture and EpigeneticsBrenna A. Market Velker, Michelle M. Denomme and Mellissa R.W. Mann André Monteiro da Rocha and Gary D. Smith 21. Human Embryo Culture Media ComparisonsThomas B. Pool, John Schoolfield and David Han 22. Embryo Culture and Monozygotic TwinningAmy E. Sparks 23. Embryo Culture and EpigeneticsBrenna A. Market Velker, Michelle M. Denomme and Mellissa R.W. Mann
Klappentext
Assisted reproductive technologies have had a profound impact on biomedical research through transgenic animals, food supply and production, as wells as genetic gain of domestic species, and treatment of human infertility. While significant advances in embryo culture have occurred over the last few decades. In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture. These include emerging methods and the impact of embryo culture on epigenetics and offspring health is presented to set the stage for future research and laboratory application involving embryo culture. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.nAuthoritative and practical, Embryo Culture: Methods and Protocols seeks to aid scientists in the further study of this crucially important field of embryology and assisted reproductive technologies.
Sets the stage for future research and laboratory application involving embryo culture
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
