Covers numerous state-of-the-art approaches to analyze epidermal differentiation
Provides step by step reproducible protocols for novices and experienced investigators
Comprises a comprehensive collection of methods in one source
Covers most recent developments in epidermal cell methodologies
Covers numerous state-of-the-art approaches to analyze epidermal differentiation
Provides step by step reproducible protocols for novices and experienced investigators
Comprises a comprehensive collection of methods in one source
Covers most recent developments in epidermal cell methodologies
Includes supplementary material: sn.pub/extras
In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology™ series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and notes on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols, Second Edition offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.
1. Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)Corina Lorz, Carmen Segrelles, Marina Garín, and Jesús M. Paramio2. Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal MicroculturesNicolas O. Fortunel, Pierre Vaigot, Emmanuelle Cadio, and Michèle T. Martin3. Growth and Stratification of Epithelial Cells in Minimal Culture ConditionsFederica Riva, Andrea Casasco, Marco Casasco, Alberto Calligaro, and Antonia Icaro Cornaglia4. Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse ModelsAlex Pines and Claude Backendorf5. Establishment of Spontaneously Immortalised Keratinocyte Lines from Wild-Type and Mutant MiceJulia Reichelt and Ingo Haase6. Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine ConditionsFrédéric Minner, Françoise Herphelin, and Yves Poumay7. Directed Differentiation of Human Embryonic Stem Cells to Epidermal ProgenitorsChristian M. Metallo, Lin Ji, Juan J. de Pablo, and Sean P. Palecek8. Expression and Analysis of Exogenous Proteins in Epidermal CellsErnest Ho, Wing Y. Chang, and Lina Dagnino9. Using siRNA Knock-Down in HaCaT Cells to Study Tranillegalscriptional Control of Epidermal Proliferation Potential Julie Wells and Xing Dai10. RNA Interference in Keratinocytes and an Organotypic Model of Human EpidermisCory L. Simpson, Shin-ichiro Kojima, and Spiro Getsios11. Scanning for Tranillegalscription Factor Binding by a Variant EMSAIgor Gurevich, Carmen Zhang, and Brian J. Aneskievich12. Chromatin Immunoprecipitation for Identifying Tranillegalscription Factor Targets in KeratinocytesKori Ortt and Satrajit Sinha13.Gene Expression Profiling of Mouse Epidermal KeratinocytesRamón García-Escudero and Jesús M. Paramio14. Analysis of Tissue-Specific Gene Expression Using Laser Capture MicrodissectionMartin Ruetze, Katrin Lochner, Stefan Gallinat, and Anja Knott15. Comprehensive Tranillegalscriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents and Cultured Keratinocytes, Using DNA Microarray ChipsDing-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, and Miroslav Blumenberg16. Molecular Profiling of the Epidermis: A Proteomics ApproachJianjun Shen and Susan M. Fischer17. Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ HybridizationMaria I. Morasso18. Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole MountsHeena Panchal, Olivia Wansbury, and Beatrice A. Howard19. Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing EpidermisCarolyn Byrne, Ariel A. Avilion, Ryan F. O´Shaughnessy, Jonathan C. Welti, and Matthew J. Hardman20. Tetracycline-Regulated Gene Expression in Transgenic Mouse EpidermisRose-Anne Romano and Satrajit Sinha21. A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal OrganizationEve Kandyba, Malcolm Hodgins, and Patricia Martin22. Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic EpidermisStéphanie Langlois, Jared M. Churko, and Dale W. Laird23. Cytokine Release in Tissue Engineered Epidermal Equivalents after Prolonged Mechanical LoadingLisette H. Cornelissen*, Debbie Bronneberg, Susan Gibbs, Carlijn V.C. Bouten, and Cees W.J. Oomens24. Three-Dimensional Human Tissue Models of Wounded SkinChristophe Egles and Jonathan A.
From the reviews of the second edition:
"This book introduces a wide variety of laboratory protocols for isolating and analyzing the differentiation potential of mammalian epidermal cells. ... The book is written for basic science investigators ... . will also be appreciated by clinicians interested in experimental dermatology and dermatopathology. ... This is an important contribution to the literature in adult stem cell biology. ... the theoretical and practical importance of this research and the diversity of topics will help this volume capture the attention of a wide audience of readers.” (Bruce A. Fenderson, Doody´s Review Service, July, 2010)
This highly practical book describes in step-by-step detail the powerful methods developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols will benefit both novices and experienced investigators.
In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications inregenerative medicine. The protocols follow the successful Methods in Molecular Biology(TM) series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and notes on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols, Second Edition offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.
Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS).- Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures.- Growth and Stratification of Epithelial Cells in Minimal Culture Conditions.- Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models.- Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice.- Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions.- Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors.- Expression and Analysis of Exogenous Proteins in Epidermal Cells.- Using siRNA Knockdown in HaCaT Cells to Study Tranillegalscriptional Control of Epidermal Proliferation Potential.- RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis.- Scanning for Tranillegalscription Factor Binding by a Variant EMSA.- Chromatin Immunoprecipitation for Identifying Tranillegalscription Factor Targets in Keratinocytes.- Gene Expression Profiling of Mouse Epidermal Keratinocytes.- Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection.- Comprehensive Tranillegalscriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and Cultured Keratinocytes Using DNA Microarray Chips.- Molecular Profiling of the Epidermis: A Proteomics Approach.- Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization.- Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts.- Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis.- Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis.- A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization.- Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis.- Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading.- Three-Dimensional Human Tissue Models of Wounded Skin.- In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis.- A Transplant Model for Human Epidermal Skin Regeneration.- Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU.- Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration.- Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay.
From the reviews of the second edition:
"This book introduces a wide variety of laboratory protocols for isolating and analyzing the differentiation potential of mammalian epidermal cells. ... The book is written for basic science investigators ... . will also be appreciated by clinicians interested in experimental dermatology and dermatopathology. ... This is an important contribution to the literature in adult stem cell biology. ... the theoretical and practical importance of this research and the diversity of topics will help this volume capture the attention of a wide audience of readers." (Bruce A. Fenderson, Doody's Review Service, July, 2010)
Inhaltsverzeichnis
1. Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
Corina Lorz, Carmen Segrelles, Marina Garín, and Jesús M. Paramio
2. Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures
Nicolas O. Fortunel, Pierre Vaigot, Emmanuelle Cadio, and Michèle T. Martin
3. Growth and Stratification of Epithelial Cells in Minimal Culture Conditions
Federica Riva, Andrea Casasco, Marco Casasco, Alberto Calligaro, and Antonia Icaro Cornaglia
4. Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models
Alex Pines and Claude Backendorf
5. Establishment of Spontaneously Immortalised Keratinocyte Lines from Wild-Type and Mutant Mice
Julia Reichelt and Ingo Haase
6. Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions
Frédéric Minner, Françoise Herphelin, and Yves Poumay
7. Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors
Christian M. Metallo, Lin Ji, Juan J. de Pablo, and Sean P. Palecek
8. Expression and Analysis of Exogenous Proteins in Epidermal Cells
Ernest Ho, Wing Y. Chang, and Lina Dagnino
9. Using siRNA Knock-Down in HaCaT Cells to Study Tranillegalscriptional Control of Epidermal Proliferation Potential
Julie Wells and Xing Dai
10. RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis
Cory L. Simpson, Shin-ichiro Kojima, and Spiro Getsios
11. Scanning for Tranillegalscription Factor Binding by a Variant EMSA
Igor Gurevich, Carmen Zhang, and Brian J. Aneskievich
12. Chromatin Immunoprecipitation for Identifying Tranillegalscription Factor Targets in Keratinocytes
Kori Ortt and Satrajit Sinha
13.Gene Expression Profiling of Mouse Epidermal Keratinocytes
Ramón García-Escudero and Jesús M. Paramio
14. Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection
Martin Ruetze, Katrin Lochner, Stefan Gallinat, and Anja Knott
15. Comprehensive Tranillegalscriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents and Cultured Keratinocytes, Using DNA Microarray Chips
Ding-Dar Lee, Jiri Zavadil, Marjana Tomic-Canic, and Miroslav Blumenberg
16. Molecular Profiling of the Epidermis: A Proteomics Approach
Jianjun Shen and Susan M. Fischer
17. Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization
Maria I. Morasso
18. Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts
Heena Panchal, Olivia Wansbury, and Beatrice A. Howard
19. Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
Carolyn Byrne, Ariel A. Avilion, Ryan F. O¿Shaughnessy, Jonathan C. Welti, and Matthew J. Hardman
20. Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis
Rose-Anne Romano and Satrajit Sinha
21. A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization
Eve Kandyba, Malcolm Hodgins, and Patricia Martin
22. Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis
Stéphanie Langlois, Jared M. Churko, and Dale W. Laird
23. Cytokine Release in Tissue Engineered Epidermal Equivalents after Prolonged Mechanical Loading
Lisette H. Cornelissen*, Debbie Bronneberg, Susan Gibbs, Carlijn V.C. Bouten, and Cees W.J. Oomens
24. Three-Dimensional Human Tissue Models of Wounded Skin
Christophe Egles and Jonathan A.
Klappentext
In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications inregenerative medicine. The protocols follow the successful Methods in Molecular Biology¿ series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and notes on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols, Second Edition offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications.
Covers numerous state-of-the-art approaches to analyze epidermal differentiation
n
Provides step by step reproducible protocols for novices and experienced investigators
n
Comprises a comprehensive collection of methods in one source
n
Covers most recent developments in epidermal cell methodologies
n
Includes supplementary material: sn.pub/extras
n
