Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
The advancements of medical technology, improvements in medical care, and increased patients´ life span make pain research and related drug development high priorities for both the research community and pharmaceutical companies. Rapid development of basic science research tools, such as techniques of flurometric labeling, genomic and proteomic high throughput screening, and genetically modified animals, promotes the swift acceleration of pain research to a stage allowing integrated investigations of pain processing mechanisms at the single cell and/or molecule level, and in a spatially and temporally controlled manner. Using multidisciplinary approaches, we can dissect the complicity of the sensory circuits connecting peripheral stimulation to maladaptive changes in the sensory pathways as well as pain perceptions at the central nervous system. Pain Research: Methods and Protocols, Second Edition provides advanced techniques and animal models that are critical for integrated pain research. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Pain Research: Methods and Protocols, Second Edition serves as an ideal guide to novice pain researchers who may not have extensive experiences in the field, or to experienced pain researchers who would like to expand their research in new directions and/or to new mechanisms in different models.
1. Advancements in Pain Research
Z. David Luo
2. Genomic Methods for Clinical and Translational Pain Research
Dan Wang, Hyungsuk Kim, Xiao-Min Wang, and Raymond Dionne
3. Two-Dimensional Gel Electrophoresis: Discovering Neuropathic Pain-Associated Synaptic Biomarkers in Spinal Cord Dorsal Horn
Om V. Singh and Yuan-Xiang Tao
4. Whole-Cell Patch-Clamp Recordings on Spinal Cord Slices
Ping Deng and Zao C. Xu
5. Whole-Cell Recording in Isolated Primary Sensory Neurons
Michael S. Gold
6. Indwelling Supradural Catheters for Induction of Facial Allodynia: Surgical Procedures, Application of Inflammatory Stimuli, and Behavioral Testing
Julie Wieseler, David Sprunger, Amanda Ellis, Steven F. Maier and Linda R. Watkins
7. An Experimental Model of Headache-Related Pain
Rebecca M. Edelmayer, Michael H. Ossipov and Frank Porreca
8. A Rodent Model of Trigeminal Neuralgia
David C. Yeomans and Mikhail Klukinov
9. New Models of Experimental Parotitis and Parotid Gland Distension in Rats
Akio Okada-Ogawa, Masamichi Shinoda, Kuniya Honda, and Koichi Iwata
10. A Rat Pain Model of Facial Cancer
Kentaro Ono, Nozomu Harano, Kiyotoshi Inenaga, and Osamu Nakanishi
11. Orofacial Pain Models and Behavior Assessment
Timothy K. Y. Kaan, Peter T. Ohara, and Luc Jasmin
12. Unilateral T13 and L1 Dorsal Root Avulsion: Methods for a Novel Model of Central Neuropathic Pain
Julie Wieseler, Amanda Ellis, Steven F. Maier, Linda R. Watkins, and Scott Falci
13. A Lumbosacral Ventral Root Avulsion Injury and Repair Model for Studies of Neuropathic Pain in Rats
Leif A. Havton
14. A Rat Chronic Pain Model of Spinal Cord Contusion Injury
Kelli Sharp, Amin Boroujerdi, Oswald Steward, and Z. David Luo
15. The Spared Nerve Injury Model of Neuropathic Pain
Marie Pertin, Romain-Daniel Gosselin, and Isabelle Decosterd
16. A New Rat Pain Model of Thrombus-Induced Ischemia
Jang-Hern Lee
17. Rat Models of Pancreatitis Pain
Karin Westlund High and Louis Vera-Portocarrero
18. The Monosodium Iodoacetate Model of Osteoarthritis Pain in The Rat
Cheryl L. Marker and James D. Pomonis
19. K/BxN Serum Transfer Arthritis as a Model of Inflammatory Joint Pain
Christina Christianson, Maripat Corr, Tony L. Yaksh, and Camilla I. Svensson
20. A New Rat Model of Bone Cancer Pain
Ruixin Zhang and Lixing Lao
21. Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury
Danielle Perret, Doo-Sik Kim, Kang-Wu Li, and Z. David Luo
From the reviews of the second edition:
"This second edition of Pain Research – Methods and Protocols is an excellent compendium of chapters outlining the most current techniques used to study the neurobiology of pain. ... Pain Research – Methods and Protocols is aimed not only at the novice researcher with little experience in the field but also at bringing new techniques and approaches to the pain research community as a whole. The clear organizational structure of the text makes this a highly achievable goal.” (Bradley Kerr, Canadian Journal of Anesthesia/Journal Canadien d'anesthésie, Vol. 60, January, 2013)
In its revised second edition, this Methods in Molecular Biology(TM) book provides advanced techniques and animal models that are critical for integrated pain research. Includes materials and reagents lists, reproducible lab protocols and troubleshooting tips.
The advancements of medical technology, improvements in medical care, and increased patients' life span make pain research and related drug development high priorities for both the research community and pharmaceutical companies. Rapid development of basic science research tools, such as techniques of flurometric labeling, genomic and proteomic high throughput screening, and genetically modified animals, promotes the swift acceleration of pain research to a stage allowing integrated investigations of pain processing mechanisms at the single cell and/or molecule level, and in a spatially and temporally controlled manner. Using multidisciplinary approaches, we can dissect the complicity of the sensory circuits connecting peripheral stimulation to maladaptive changes in the sensory pathways as well as pain perceptions at the central nervous system. Pain Research: Methods and Protocols, Second Edition provides advanced techniques and animal models that are critical for integrated pain research. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and accessible, Pain Research: Methods and Protocols, Second Edition serves as an ideal guide to novice pain researchers who may not have extensive experiences in the field, or to experienced pain researchers who would like to expand their research in new directions and/or to new mechanisms in different models.
Advancements in Pain Research.- Genomic Methods for Clinical and Translational Pain Research.- Two-Dimensional Gel Electrophoresis: Discovering Neuropathic Pain-Associated Synaptic Biomarkers in Spinal Cord Dorsal Horn.- Whole-Cell Patch-Clamp Recordings on Spinal Cord Slices.- Whole-Cell Recording in Isolated Primary Sensory Neurons.- Indwelling Supradural Catheters for Induction of Facial Allodynia: Surgical Procedures, Application of Inflammatory Stimuli, and Behavioral Testing.- An Experimental Model of Headache-Related Pain.- A Rodent Model of Trigeminal Neuralgia.- New Models of Experimental Parotitis and Parotid Gland Distension in Rats.- A Rat Pain Model of Facial Cancer.- Orofacial Pain Models and Behavior Assessment.- Unilateral T13 and L1 Dorsal Root Avulsion: Methods for a Novel Model of Central Neuropathic Pain.- A Lumbosacral Ventral Root Avulsion Injury and Repair Model for Studies of Neuropathic Pain in Rats.- A Rat Chronic Pain Model of Spinal Cord Contusion Injury.- The Spared Nerve Injury Model of Neuropathic Pain.- A New Rat Pain Model of Thrombus-Induced Ischemia.- Rat Models of Pancreatitis Pain.- The Monosodium Iodoacetate Model of Osteoarthritis Pain in The Rat.- K/BxN Serum Transfer Arthritis as a Model of Inflammatory Joint Pain.- A New Rat Model of Bone Cancer Pain.- Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury.
From the reviews of the second edition:
"This second edition of Pain Research - Methods and Protocols is an excellent compendium of chapters outlining the most current techniques used to study the neurobiology of pain. ... Pain Research - Methods and Protocols is aimed not only at the novice researcher with little experience in the field but also at bringing new techniques and approaches to the pain research community as a whole. The clear organizational structure of the text makes this a highly achievable goal." (Bradley Kerr, Canadian Journal of Anesthesia/Journal Canadien d'anesthésie, Vol. 60, January, 2013)
Inhaltsverzeichnis
1. Advancements in Pain Research Z. David Luo 2. Genomic Methods for Clinical and Translational Pain Research Dan Wang, Hyungsuk Kim, Xiao-Min Wang, and Raymond Dionne 3. Two-Dimensional Gel Electrophoresis: Discovering Neuropathic Pain-Associated Synaptic Biomarkers in Spinal Cord Dorsal Horn Om V. Singh and Yuan-Xiang Tao 4. Whole-Cell Patch-Clamp Recordings on Spinal Cord Slices Ping Deng and Zao C. Xu 5. Whole-Cell Recording in Isolated Primary Sensory Neurons Michael S. Gold 6. Indwelling Supradural Catheters for Induction of Facial Allodynia: Surgical Procedures, Application of Inflammatory Stimuli, and Behavioral Testing Julie Wieseler, David Sprunger, Amanda Ellis, Steven F. Maier and Linda R. Watkins 7. An Experimental Model of Headache-Related Pain Rebecca M. Edelmayer, Michael H. Ossipov and Frank Porreca 8. A Rodent Model of Trigeminal Neuralgia David C. Yeomans and Mikhail Klukinov 9. New Models of Experimental Parotitis and Parotid Gland Distension in Rats Akio Okada-Ogawa, Masamichi Shinoda, Kuniya Honda, and Koichi Iwata 10. A Rat Pain Model of Facial Cancer Kentaro Ono, Nozomu Harano, Kiyotoshi Inenaga, and Osamu Nakanishi 11. Orofacial Pain Models and Behavior Assessment Timothy K. Y. Kaan, Peter T. Ohara, and Luc Jasmin 12. Unilateral T13 and L1 Dorsal Root Avulsion: Methods for a Novel Model of Central Neuropathic Pain Julie Wieseler, Amanda Ellis, Steven F. Maier, Linda R. Watkins, and Scott Falci 13. A Lumbosacral Ventral Root Avulsion Injury and Repair Model for Studies of Neuropathic Pain in Rats Leif A. Havton 14. A Rat Chronic Pain Model of Spinal Cord Contusion Injury Kelli Sharp, Amin Boroujerdi, Oswald Steward, and Z. David Luo 15. The Spared Nerve Injury Model of Neuropathic Pain Marie Pertin, Romain-Daniel Gosselin, and Isabelle Decosterd 16. A New Rat Pain Model of Thrombus-Induced Ischemia Jang-Hern Lee 17. Rat Models of Pancreatitis Pain Karin Westlund High and Louis Vera-Portocarrero 18. The Monosodium Iodoacetate Model of Osteoarthritis Pain in The Rat Cheryl L. Marker and James D. Pomonis 19. K/BxN Serum Transfer Arthritis as a Model of Inflammatory Joint Pain Christina Christianson, Maripat Corr, Tony L. Yaksh, and Camilla I. Svensson 20. A New Rat Model of Bone Cancer Pain Ruixin Zhang and Lixing Lao 21. Exposure of the Dorsal Root Ganglion to Pulsed Radiofrequency Current in a Neuropathic Pain Model of Peripheral Nerve Injury Danielle Perret, Doo-Sik Kim, Kang-Wu Li, and Z. David Luo
Klappentext
The advancements of medical technology, improvements in medical care, and increased patients¿ life span make pain research and related drug development high priorities for both the research community and pharmaceutical companies. Rapid development of basic science research tools, such as techniques of flurometric labeling, genomic and proteomic high throughput screening, and genetically modified animals, promotes the swift acceleration of pain research to a stage allowing integrated investigations of pain processing mechanisms at the single cell and/or molecule level, and in a spatially and temporally controlled manner. Using multidisciplinary approaches, we can dissect the complicity of the sensory circuits connecting peripheral stimulation to maladaptive changes in the sensory pathways as well as pain perceptions at the central nervous system. Pain Research: Methods and Protocols, Second Edition provides advanced techniques and animal models that are critical for integrated pain research. Written in the highly successful Methods in Molecular Biology¿ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.n nAuthoritative and accessible, Pain Research: Methods and Protocols, Second Edition serves as an ideal guide to novice pain researchers who may not have extensive experiences in the field, or to experienced pain researchers who would like to expand their research in new directions and/or to new mechanisms in different models.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
