Chloroplast Research in Arabidopsis
(Englisch)
Methods and Protocols, Volume I
Jarvis, R. Paul

Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis.- Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy.- Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins.- Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts.- Visualisation of Stromules on Arabidopsis Plastids.- Analysis of Chloroplast Movement and Relocation in Arabidopsis.- Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots.- Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy.- Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method.- Isolation, Quantification, and Analysis of Chloroplast DNA.- Measurement of Tranillegalscription Rates in Arabidopsis Chloroplasts.- Studying the Structure and Processing of Chloroplast Tranillegalscripts.- In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts.- Studying Translation in Arabidopsis Chloroplasts.- Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts.- In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins.- Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays.- Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions.- Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays.- Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction.- Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases.

Inhaltsverzeichnis

Part I: Genetics, Cytology, and In Vivo Analysis 1. Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis            Enrique López-Juez and Alison Hills 2. Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy            Kevin Pyke 3. Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins            Stanislav Vitha and Katherine W. Osteryoung 4. Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts            Dong Wook Lee and Inhwan Hwang 5. Visualisation of Stromules on Arabidopsis Plastids            John C. Gray, James A. Sullivan, and Christine A. Newell 6. Analysis of Chloroplast Movement and Relocation in Arabidopsis            Masamitsu Wada and Sam-Geun Kong 7. Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots            John Stanga, Allison Strohm, and Patrick H. Masson 8. Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy            Stefan Hyman and R. Paul Jarvis 9. Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method            Kerry Ann Lutz, Arun Azhagiri, and Pal Maliga Part II: Gene Expression and Protein Accumulation 10. Isolation, Quantification, and Analysis of Chloroplast DNA            Beth A. Rowan and Arnold J. Bendich 11. Measurement of Tranillegalscription Rates in Arabidopsis Chloroplasts            Yan O. Zubo, Thomas Börner, and Karsten Liere 12. Studying the Structure and Processing of Chloroplast Tranillegalscripts            Alice Barkan 13. In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts            Toshiharu Shikanai and Kenji Okuda 14. Studying Translation in Arabidopsis Chloroplasts            Paolo Pesaresi 15. Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts            Lars L.E. Sjögren and Adrian K. Clarke Part III: Protein Transport, Localization, and Topology 16. In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins            Sandra Tanz and Ian Small 17. Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays            Henrik Aronsson and R. Paul Jarvis 18. Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions            Hitoshi Inoue, Fei Wang, Takehito Inaba, and Danny J. Schnell 19. Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays            Thomas Bals and Danja Schünemann 20. Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction            Chiung-Chih Chu and Hsou-min Li 21. Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases            John Froehlich

Klappentext

Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume I explores topics such as genetics, cytology, in vivo analysis, gene expression, and protein accumulation, as well as protein transport, localization, and topology.  Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.n Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Brings together the most important and current techniques in chloroplast research through the unifying lens of the Arabidopsis model system

Features subjects like genetics, cytology, and protein studies

Includes expert tips and implementation advice to guarantee ideal experimental results