-Provides an up-to-date variety of protocols covering all aspects in thorough, clear detail -Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand upon them -Includes tips from the experts highlighting potential methodological pitfalls in order to ensure successful implementation
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
1. Cell-Based Microarrays: OverviewElla Palmer2. Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated MicroplateMeenal Soni and Fang Lai3. Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic AssaysElla Palmer and Tom C. Freeman4. A Novel Fluorescent Tranillegalscriptional Reporter for Cell-Based Microarray AssaysTanya M. Redmond and Michael D. Uhler5. High-Throughput Subcellular Protein Localization Using Transfected-Cell ArraysYuhui Hu and Michal Janitz6. Cell Arrays for the Measurement of Organelle Dynamics in Living CellsHolger Erfle, Tautvydas Lisauskas, Christoph Claas, Jürgen Reymann, and Vytaute Starkuviene7. High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based MicroarraysWei Niu, G. Traver Hart, and Edward M. Marcotte8. Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA)Hansjürgen Volkmer and Frank Weise9. Reverse Transfected Cell Microarrays in Infectious Disease ResearchAndreas Konrad, Ramona Jochmann, Elisabeth Kuhn, Elisabeth Naschberger, Priya Chudasama, and Michael Stürzl10. Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain AntibodiesBaochuan Lin and James B. Delehanty11. Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance ImagingYoann Roupioz, Sarah Milgram, André Roget, and Thierry Livache12. Immobilized Culture and Transfection Microarray of Nonadherent CellsSatoshi Yamaguchi, Erika Matsunuma, and Teruyuki Nagamune13. Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell MicroarraysAndrew L. Hook, Nicolas H. Voelcker, and Helmut Thissen14. Polymer Microarrays for Cellular High-Content ScreeningSalvatore Pernagallo and Juan J. Diaz-Mochon15. High-Throughput Analyses of Gene Functions on a Cell Chip by ElectroporationKoichi Kato and Hiroo Iwata16. Microfluidic Image CytometryKen-ichiro Kamei, Jing Sun, Hsian-Rong Tseng, and Robert Damoiseaux
As a high-throughput method of analyzing gene function, cell-based microarrays have proven of vital importance. Here, experts provide an up-to-date assessment of these microarrays and meticulous coverage of all their aspects, including emerging technology.
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
Cell-Based Microarrays: Overview.- Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate.- Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays.- A Novel Fluorescent Tranillegalscriptional Reporter for Cell-Based Microarray Assays.- High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays.- Cell Arrays for the Measurement of Organelle Dynamics in Living Cells.- High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays.- Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA).- Reverse Transfected Cell Microarrays in Infectious Disease Research.- Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies.- Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging.- Immobilized Culture and Transfection Microarray of Nonadherent Cells.- Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays.- Polymer Microarrays for Cellular High-Content Screening.- High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation.- Microfluidic Image Cytometry.
Inhaltsverzeichnis
1. Cell-Based Microarrays: Overview
Ella Palmer
2. Cell-Based Co-Transfection Microarrays for Use with HEK293T Cells on a Poly D-Lysine Polystyrene Coated Microplate
Meenal Soni and Fang Lai
3. Large-Scale Cell-Based Microarrays and Their Use with HEK293T Cells and Downstream Apoptotic Assays
Ella Palmer and Tom C. Freeman
4. A Novel Fluorescent Tranillegalscriptional Reporter for Cell-Based Microarray Assays
Tanya M. Redmond and Michael D. Uhler
5. High-Throughput Subcellular Protein Localization Using Transfected-Cell Arrays
Yuhui Hu and Michal Janitz
6. Cell Arrays for the Measurement of Organelle Dynamics in Living Cells
Holger Erfle, Tautvydas Lisauskas, Christoph Claas, Jürgen Reymann, and Vytaute Starkuviene
7. High-Throughput Immunofluorescence Microscopy Using Yeast Spheroplast Cell-Based Microarrays
Wei Niu, G. Traver Hart, and Edward M. Marcotte
8. Cell-Based Microarrays of Infectious Adenovirus Encoding Short Hairpin RNA (shRNA)
Hansjürgen Volkmer and Frank Weise
9. Reverse Transfected Cell Microarrays in Infectious Disease Research
Andreas Konrad, Ramona Jochmann, Elisabeth Kuhn, Elisabeth Naschberger, Priya Chudasama, and Michael Stürzl
10. Transfected Cell Microarrays for the Expression of Membrane-Displayed Single-Chain Antibodies
Baochuan Lin and James B. Delehanty
11. Blood Cell Capture on Antibody Micro-Arrays and Monitoring of the Cell Capture Using Surface Plasmon Resonance Imaging
Yoann Roupioz, Sarah Milgram, André Roget, and Thierry Livache
12. Immobilized Culture and Transfection Microarray of Nonadherent Cells
Satoshi Yamaguchi, Erika Matsunuma, and Teruyuki Nagamune
13. Plasma Polymer and PEG-Based Coatings for DNA, Protein, and Cell Microarrays
Andrew L. Hook, Nicolas H. Voelcker, and Helmut Thissen
14. Polymer Microarrays for Cellular High-Content Screening
Salvatore Pernagallo and Juan J. Diaz-Mochon
15. High-Throughput Analyses of Gene Functions on a Cell Chip by Electroporation
Koichi Kato and Hiroo Iwata
16. Microfluidic Image Cytometry
Ken-ichiro Kamei, Jing Sun, Hsian-Rong Tseng, and Robert Damoiseaux
Klappentext
As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.nnComprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.
Provides an up-to-date variety of protocols covering all aspects in thorough, clear detail -Presents readers with clear step-by-step protocols to allow them to set up difficult techniques and expand upon them -Includes tips from the experts highlighting potential methodological pitfalls in order to ensure successful implementation
Includes supplementary material: sn.pub/extras
