Synthesizes electroporation, electroporation chemotherapy, and electroporation gene therapy, with a focus on the latter
Contains step-by-step instructions that ensure successful results
Provides notes on troubleshooting and avoiding known pitfalls
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In this book, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. The book synthesizes electroporation, electroporation chemotherapy, and electroporation gene therapy, with a focus on the latter. Following the successful Methods in Molecular Biology™ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.
Dec 1st, 2006 Editor: Shulin Li (USA) Book title: Electroporation protocols: Experimental and Clinical Gene Medicine Publisher: The Humana Press, Inc Preface (Shulin Li, no need to fit chapter format) I. Basic concepts of DNA transfer via electroporation (review chapters, no need to fit chapter format for all the chapters in this section) Application of Electroporation gene therapy: past, current, and future (Lluis M. Mir) Mechanism by which electroporation mediates DNA migration and entry into cells and the targeted tissues (Marie-Pierre Rols) Applicator and electrode design for in vivo DNA delivery by electroporation (Dietmar Rabussay) Electrode assemblies used for electroporation of cultured cells (Leda Raptis and Kevin L. Firth) Formulations for DNA delivery via electroporation in vivo (Khursheed Anwer) Overview of drug delivery and alternative methods to electroporation (Sek-Wen Hui) Nano-Electroporation—A First Look (Raji Sundararajan) II. In vitro targeted gene delivery via electroporation Nucleofection of Human Embryonic Stem Cells (Henrike Siemen, Lars Nolden, Stefanie Terstegge, Philipp Koch, and Oliver Brustle) Delivery of whole tumor lysate into dendritic cells for cancer vaccination (Linda N. Liu, Rama Shivakumar, Cornell Allan, and Joseph C. Fratantoni) Delivery of tumor antigen encoding mRNA into dendritic cells for vaccination (Annelies Michiels, Sandra Tuyaerts, Aude Bonehill, Carlo Heirman, Jurgen Corthals, and Kris Thielemans) Delivery of DNA into natural killer cells for immunotherapy (Kathrin Schoenberg, Hans-Ingo Trompeter, and Markus Uhrberg) Electroporation ofadherent cells in situ for the study of signal transduction and gap junctional communication (Leda Raptis, Adina Vultur, Heather L. Brownell, Evangelia Tomai, Aikaterini Anagnostopoulou, and Kevin L. Firth) Delivery of DNA into adipocytes within adipose tissue (James G. Granneman) III: In vivo targeted gene delivery via electroporation Delivery of DNA into muscle for treating systemic diseases: advantage and challenge (review chapter, no need to fit chapter format) (Capucine Trollet, Daniel Scherman, Pascal Bigey) Delivery DNA into skeletal muscle in large animals (Patricia A. Brown, Amir S. Khan, and Ruxandra Draghia-Akli) Delivery DNA into skin via electroporation (Babu M. Medi and Jagdish Singh) Electroporation-mediated Gene Delivery to the Lungs(Rui Zhou, James E. Norton, and David A. Dean) Delivery DNA into bladder via electroporation (Masaki Yoshida, Hitoshi Iwashita, Masayuki Otani, Koichi Masunaga, and Akito Inadome) Analysis of gene function in the retina (Takahiko Matsuda and Constance L. Cepko) Optical in vivo imaging of electrically mediated delivery of siRNA into muscle for gene function analysis (Muriel Golzio and Justin Teissie) Electroporation of adult zebrafish (N. Madhusudhana Rao, K. Murali Rambabu, and S. Harinarayana Rao) IV: Treatment of cancer via electroporation gene therapy Flow electroporation with pulsed electric fields for purging tumor cells (Abie Craiu and David Scadden) Delivery of DNA into tumors (Shulin Li) Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumor in Dogs (Jeffry Cutrera, Marina Torrero, Keijiro Shiomitsu, Neal Mauldin, and Shulin Li)
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinical and Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology™ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.
Ideal for beginners and essential for the more knowledgable, Electroporation Protocols: Preclinical and Clinical Gene Medicine collects a comprehensive set of the newest discoveries and applications in a revolutionary field that offers so much to our fight against disease.
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology(TM) series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.
Basic Concepts of DNA Transfer via Electroporation.- Application of Electroporation Gene Therapy: Past, Current, and Future.- Mechanism by Which Electroporation Mediates DNA Migration and Entry into Cells and Targeted Tissues.- Applicator and Electrode Design for In Vivo DNA Delivery by Electroporation.- Electrode Assemblies Used for Electroporation of Cultured Cells.- Formulations for DNA Delivery via Electroporation In Vivo.- Overview of Drug Delivery and Alternative Methods to Electroporation.- Nanoelectroporation: A First Look.- In Vitro Targeted Gene Delivery via Electroporation.- Nucleofection of Human Embryonic Stem Cells.- Delivery of Whole Tumor Lysate into Dendritic Cells for Cancer Vaccination.- Delivery of Tumor-Antigen-Encoding mRNA into Dendritic Cells for Vaccination.- Delivery of DNA into Natural Killer Cells for Immunotherapy.- Electroporation of Adherent Cells In Situ for the Study of Signal Transduction and Gap Junctional Communication.- Delivery of DNA into Adipocytes within Adipose Tissue.- In Vivo Targeted Gene Delivery via Electroporation.- Delivery of DNA into Muscle for Treating Systemic Diseases: Advantages and Challenges.- Delivery of DNA into Skeletal Muscle in Large Animals.- Delivery of DNA into Skin via Electroporation.- Electroporation-Mediated Gene Delivery to the Lungs.- Delivery of DNA into Bladder via Electroporation.- Analysis of Gene Function in the Retina.- Optical In Vivo Imaging of Electrically Mediated Delivery of siRNA into Muscle for Gene Function Analysis.- Electroporation of Adult Zebrafish.- Treatment of Cancer via Electroporation Gene Therapy.- Flow Electroporation with Pulsed Electric Fields for Purging Tumor Cells.- Delivery of DNA into Tumors.- Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumors in Dogs.- Systemic IL-12 Gene Therapy for Treating Malignancy via Intramuscular Electroporation.- Treatment of SCCVII Tumors with Systemic Chemotherapy and Interleukin-12 Gene Therapy Combination.- Electroporation for Drug and Gene Delivery in the Clinic: Doctors Go Electric.- IL-2 Plasmid Electroporation: From Preclinical Studies to Phase I Clinical Trial.- Treatment of Other Diseases via Electroporation Gene Therapy.- Factor IX Gene Therapy for Hemophilia.- KGF-1 for Wound Healing in Animal Models.- Hepatocyte Growth Factor Gene Therapy for Hypertension.- Electroporation of Corrective Nucleic Acids (CNA) In Vivo to Promote Gene Correction in Dystrophic Muscle.- Gene Delivery to Dystrophic Muscle.- Effect of Electroporation on Cardiac Electrophysiology.- Muscle and Fat Mass Modulation in Different Clinical Models.- Applications of Electroporation for DNA Vaccination.- DNA Vaccination for Prostate Cancer.- HER2/neu DNA Vaccination for Breast Tumors.- Electroporation-Mediated HBV DNA Vaccination in Primate Models.- Taking Electroporation-Based Delivery of DNA Vaccination into Humans: A Generic Clinical Protocol.- Production of Monoclonal Antibody by DNA Immunization with Electroporation.
Inhaltsverzeichnis
Dec 1st, 2006
Editor: Shulin Li (USA)
Book title: Electroporation protocols: Experimental and Clinical Gene Medicine Publisher: The Humana Press, Inc
Preface (Shulin Li, no need to fit chapter format)
I. Basic concepts of DNA transfer via electroporation (review chapters, no need to fit chapter format for all the chapters in this section)
Application of Electroporation gene therapy: past, current, and future (Lluis M. Mir)
Mechanism by which electroporation mediates DNA migration and entry into cells and the targeted tissues (Marie-Pierre Rols)
Applicator and electrode design for in vivo DNA delivery by electroporation (Dietmar Rabussay)
Electrode assemblies used for electroporation of cultured cells (Leda Raptis and Kevin L. Firth)
Formulations for DNA delivery via electroporation in vivo (Khursheed Anwer)
Overview of drug delivery and alternative methods to electroporation (Sek-Wen Hui)
Nano-Electroporation-A First Look (Raji Sundararajan)
II. In vitro targeted gene delivery via electroporation
Nucleofection of Human Embryonic Stem Cells (Henrike Siemen, Lars Nolden, Stefanie Terstegge, Philipp Koch, and Oliver Brustle)
Delivery of whole tumor lysate into dendritic cells for cancer vaccination (Linda N. Liu, Rama Shivakumar, Cornell Allan, and Joseph C. Fratantoni)
Delivery of tumor antigen encoding mRNA into dendritic cells for vaccination (Annelies Michiels, Sandra Tuyaerts, Aude Bonehill, Carlo Heirman, Jurgen Corthals, and Kris Thielemans)
Delivery of DNA into natural killer cells for immunotherapy (Kathrin Schoenberg, Hans-Ingo Trompeter, and Markus Uhrberg)
Electroporation of adherent cells in situ for the study of signal transduction and gap junctional communication (Leda Raptis, Adina Vultur, Heather L. Brownell, Evangelia Tomai, Aikaterini Anagnostopoulou, and Kevin L. Firth)
Delivery of DNA into adipocytes within adipose tissue (James G. Granneman)
III: In vivo targeted gene delivery via electroporation
Delivery of DNA into muscle for treating systemic diseases: advantage and challenge (review chapter, no need to fit chapter format) (Capucine Trollet, Daniel Scherman, Pascal Bigey)
Delivery DNA into skeletal muscle in large animals (Patricia A. Brown, Amir S. Khan, and Ruxandra Draghia-Akli)
Delivery DNA into skin via electroporation (Babu M. Medi and Jagdish Singh)
Electroporation-mediated Gene Delivery to the Lungs(Rui Zhou, James E. Norton, and David A. Dean)
Delivery DNA into bladder via electroporation (Masaki Yoshida, Hitoshi Iwashita, Masayuki Otani, Koichi Masunaga, and Akito Inadome)
Analysis of gene function in the retina (Takahiko Matsuda and Constance L. Cepko)
Optical in vivo imaging of electrically mediated delivery of siRNA into muscle for gene function analysis (Muriel Golzio and Justin Teissie)
Electroporation of adult zebrafish (N. Madhusudhana Rao, K. Murali Rambabu, and S. Harinarayana Rao)
IV: Treatment of cancer via electroporation gene therapy
Flow electroporation with pulsed electric fields for purging tumor cells (Abie Craiu and David Scadden)
Delivery of DNA into tumors (Shulin Li)
Intratumoral Bleomycin and IL-12 Electrochemogenetherapy for Treating Head and Neck Tumor in Dogs (Jeffry Cutrera, Marina Torrero, Keijiro Shiomitsu, Neal Mauldin, and Shulin Li)
Systemic IL-12 gene therapy for treating malignancy via intramuscular electroporation (Shiguo Zhu and Shulin Li)
Treatment of SCCVII tumors with systemic chemotherapy and Interleukin-12 gene therapy combination (Marina Torrero and Shulin Li)
Electroporation for gene delivery in the clinic: Doctors go electric (Julie Gehl, Review, no chapter format is needed, Color print permitted)
IL-2 plasmid DNA electroporation: from preclinical studies to Phase 1 clinical trial (Holly M. Horton, Peggy A. Lalor and Alain P. Rolland)
V: Treatment of other diseases via electroporation gene therapy
Factor IX gene therapy for hemophilia (Jason G. Fewell)
KGF1 for wound healing in animal models (Guy P. Marti, Parsa Mohebi, Lixin Liu, Jiaai Wang, Tomoharu Miyashita, John W. Harmon)
Hepatocyte growth factor gene therapy for hypertension (Kazuo Komamura, Jun-ichi Miyazaki, Enyu Imai, Kunio Matsumoto, Toshikazu Nakamura, Masatsugu Hori)
Electroporation of Corrective Nucleic Acids (CNA) in vivo to promote Gene Correction in dystrophic muscle (Robert M.I. Kapsa, Sharon H. A. Wong, and Anita F. Quigley)
Gene delivery to dystrophic muscle (Kim E. Wells, Jill McMahon, Helen Foster, Aurora Ferrer, and Dominic J. Wells)
Effect of electroporation on cardiac electrophysiology (Review chapter, no need to fit chapter format) (Vadim V. Federov, Vladimir P. Nikolski, Igor R. Efimov)
Muscle and fat mass modulation in different clinical models (review chapter, no need to fit chapter format) (Ruxandra Draghia-Akli and Amir S. Khan)
VI: Applications of electroporation for DNA vaccination
DNA vaccination for prostate cancer (Anna-Karin Roos, Alan King, Pavel Pisa)
HER2/neu DNA vaccination for breast cancer (Arianna Smorlesi, Francesca Papalini, Sara Pierpaoli, and Mauro Provinciali)
Electroporation mediated HBV DNA vaccination in primate models (Yong-Gang Zhao and Yuhong Xu)
Taking electroporation based delivery of DNA vaccination into humans: A generic clinical protocol (Torunn Elisabeth Tjelle, Dietmar Rabussay, Christian Ottensmeier, Iacob Mathiesen, Rune Kjeken)
Production of Monoclonal Antibody by DNA Immunization with Electroporation (Chua Kaw Yan, John Donnie A. Ramos, Cheong Nge)
Klappentext
At the time of the lightbulb's invention, not even the most imaginative thinkers could have predicted the prevention and treatment of disease through genes delivered by electric fields. In Electroporation Protocols: Preclinicaland Clinical Gene Medicine, top researchers provide hands-on protocols describing the process of non-viral delivery of genetic materials such as DNA, oligo-DNA, and RNA into targeted immune cells, tissues, and model animals in order to better treat disease. Following the successful Methods in Molecular Biology¿ series format, each method and protocol offers step-by-step, readily reproducible laboratory instructions, with the benefit of several introductory reviews to aid investigators new to this exciting technology.
