Includes supplementary material: sn.pub/extras
Inflammation has been described as the basis of many pathologies of human disease. When one considers the updated signs of inflammation, they would be vasodilation, cell migration, and, in the case of chronic inflam- tion, cell proliferation, often with an underlying autoimmune basis. Gen- ally, inflammation may be divided into acute, chronic, and autoimmune, - though the editors believe that most, if not all, chronic states are often the result of an autoimmune response to an endogenous antigen. Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well. The lack of advances in classical inflammatory diseases, such as rh- matoid arthritis, may in part arise from a failure to classify the disease into different forms. That different forms exist is exemplified in patients with d- fering responses to existing antiinflammatory drugs, ranging from nonresponders to very positive responders for a particular nonsteroidal an- inflammatory drug (NSAID). Though researchers have progressively unr- eled the mechanisms, the story is far from complete. It should also be noted that the inflammatory response is part of the innate immune response, or to use John Hunter´s words in 1795, "inflammation is a salutary response.” That may be applied in particular to the defensive response to invading micro- ganisms.
Part I. In Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory Drugs Key Stages in the Acute Inflammatory Response and Their Relevance as Therapeutic Targets: Introduction to Part 1 Paul G. Winyard IkB Kinase and NF-kB Signaling in Response to Pro-Inflammatory Cytokines Mireille Delhase Screening for Inhibitors of Tranillegalscription Factors, Using Luciferase Reporter Gene Expression in Transfected Cells Deborah Phippard and Anthony M. Manning Adhesion Molecule Expression on Cytokine-Stimulated Human Endothelial Cells Susan L. Cuvelier and Kamala D. Patel Phagocytosis by Inflammatory Phagocytes: Experimental Strategies for Stimulation and Quantification M. Rachel Morris, Sharon Dewitt, Iraj Laffafian, and Maurice B. Hallett Cytosolic Ca2+ Measurement and Imaging in Inflammatory Cells Sharon Dewitt, Iraj Laffafian, M. Rachel Morris, and Maurice B. Hallett Detection and Visualization of Oxidase Activity in Phagocytes Maurice B. Hallett, Caroline Cole, and Sharon Dewitt Measurement of Complement Activation Tom Eirik Mollnes Measurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using Zymography Linda Troeberg and Hideaki Nagase Measurement of Aggrecanase-Generated Interglobular Domain Catabolites in the Medium and Extracts of Cartilage Explants Using Western Blot Analysis Clare E. Hughes, Christopher B. Little, and Bruce Caterson In Vitro Model of Human Articular Cartilage Degradation William D. Shingleton Part II. In Vivo Models of Inflammation In Vivo Models of Inflammation: Introduction to Part 2 Derek A. Willoughby Carrageenan-Induced Paw Edema in the Rat and Mouse Christopher J. Morris Pleural Models of Inflammation: Immune and Nonimmune Adrian R. Moore Models of Acute Inflammation in the Ear Miklós Gábor Migration of Specific Leukocyte Subsets in Response to Cytokine or Chemokine Application In Vivo Mauro Perretti and Stephen J. Getting Inflammatory Joint Disease: Clinical, Histological, and Molecular Parameters of Acute and Chronic Inflammation and Tissue Destruction Nancy L. McCartney-Francis, James Chan, and Sharon M. Wahl The Assessment of Inflammation, Cartilage Matrix, and Bone Loss in Experimental Monoarticular Arthritis of the Rat Michael P. Seed Collagen-Induced Arthritis Adrian R. Moore Air-Pouch Models of Inflammation and Modifications for the Study of Granuloma-Mediated Cartilage Degradation Paul Colville-Nash and Toby Lawrence Quantitative Analysis of Angiogenesis Using the Murine Chronic Granulomatous Air Pouch Chandan A. S. Alam Models of Coronary Artery Occlusion and Reperfusion for the Discovery of Novel Antiischemic and Antiinflammatory Drugs for the Heart Nicole S. Wayman, Michelle C. McDonald, Prabal K. Chatterjee, and Christoph Thiemermann Assessment of Anticolitic Drugs in the Trinitrobenzene Sulfonic Acid (TNBS) Rat Model of Inflammatory Bowel Disease Brendan J. R. Whittle, Maryan Cavicchi, and Dominique Lamarque An In Vivo Model of Ischemia/Reperfusion and Inflammation of the Kidneys of the Rat Prabal K. Chatterjee and Christoph Thiemermann In Vivo Models of Inflammation: Immune Rejection and Skin Transplantation In Vivo Isabelle Binet and Kathryn J. Wood Wound Healing: A Model of Dermal Wound Repair Annette Tomlinson and Mark W. J. Ferguson Part III. Pharmacodynamic Endpoints in Experimental Models and in Clinical Studies in Humans An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans: Introduction to Part 3 David R. Blake and Gordon J.
Because inflammation is involved in many chronic human diseases, its proper understanding may provide important clues to new therapeutic targets. In Inflammation Protocols, highly skilled experimenters present key techniques for the multidisciplinary study of inflammation in such conditions as inflammatory bowel disease, rheumatoid arthritis, renal disease, and cardiovascular disease. Described in step-by-step detail to ensure experimental success, the techniques are focused on the research and development of potential new antiinflammatory drugs in active target areas. The protocols are suitable for many current areas of drug discovery research, including tranillegalscription factors, cytokines, adhesion molecules, cyclooxygenase-2 (COX-2) inhibitors, free radicals, nitric oxide synthases, complement activation, angiogenesis, wound healing, immune rejection, and metalloproteinases. The methods provide in vitro systems for studying inflammation, in vivo models, and relevant pharmacodynamic measurements for the assessment of antiinflammatory compounds. Each protocol contains notes about potential pitfalls and tips on how to avoid failure. Overview chapters at the start of each section review the significance and potential limitations of the protocols presented.
Cutting-edge and highly practical, Inflammation Protocols offers experimental and clinical researchers a premier collection of readily reproducible techniques not only for evaluating inflammatory disease processes, but also for the discovery of new drugs for treating chronic human inflammatory diseases today.
Inflammation has been described as the basis of many pathologies of human disease. When one considers the updated signs of inflammation, they would be vasodilation, cell migration, and, in the case of chronic inflam- tion, cell proliferation, often with an underlying autoimmune basis. Gen- ally, inflammation may be divided into acute, chronic, and autoimmune, - though the editors believe that most, if not all, chronic states are often the result of an autoimmune response to an endogenous antigen. Thus, a proper understanding of the inflammatory basis may provide clues to new therap- tic targets not only in classical inflammatory diseases, but atherosclerosis, cancer, and ischemic heart disease as well. The lack of advances in classical inflammatory diseases, such as rh- matoid arthritis, may in part arise from a failure to classify the disease into different forms. That different forms exist is exemplified in patients with d- fering responses to existing antiinflammatory drugs, ranging from nonresponders to very positive responders for a particular nonsteroidal an- inflammatory drug (NSAID). Though researchers have progressively unr- eled the mechanisms, the story is far from complete. It should also be noted that the inflammatory response is part of the innate immune response, or to use John Hunter's words in 1795, "inflammation is a salutary response." That may be applied in particular to the defensive response to invading micro- ganisms.
In Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory Drugs.- Key Stages in the Acute Inflammatory Response and Their Relevance as Therapeutic Targets.- I?B Kinase and NF-?B Signaling in Response to Pro-Inflammatory Cytokines.- Screening for Inhibitors of Tranillegalscription Factors Using Luciferase Reporter Gene Expression in Transfected Cells.- Adhesion Molecule Expression on Cytokine-Stimulated Human Endothelial Cells.- Phagocytosis by Inflammatory Phagocytes.- Cytosolic Ca2+ Measurement and Imaging in Inflammatory Cells.- Detection and Visualization of Oxidase Activity in Phagocytes.- Measurement of Complement Activation.- Measurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using Zymography.- Measurement of Aggrecanase-Generated Interglobular Domain Catabolites in the Medium and Extracts of Cartilage Explants Using Western Blot Analysis.- In Vitro Model of Human Articular Cartilage Degradation.- In Vivo Models of Inflammation.- In Vivo Models of Inflammation.- Carrageenan-Induced Paw Edema in the Rat and Mouse.- Pleural Models of Inflammation.- Models of Acute Inflammation in the Ear.- Migration of Specific Leukocyte Subsets in Response to Cytokine or Chemokine Application In Vivo.- Inflammatory Joint Disease.- The Assessment of Inflammation, Cartilage Matrix, and Bone Loss in Experimental Monoarticular Arthritis of the Rat.- Collagen-Induced Arthritis.- Air-Pouch Models of Inflammation and Modifications for the Study of Granuloma-Mediated Cartilage Degradation.- Quantitative Analysis of Angiogenesis Using the Murine Chronic Granulomatous Air Pouch.- Models of Coronary Artery Occlusion and Reperfusion for the Discovery of Novel Antiischemic and Antiinflammatory Drugs for the Heart.-Assessment of Anticolitic Drugs in the Trinitrobenzene Sulfonic Acid (TNBS) Rat Model of Inflammatory Bowel Disease.- An In Vivo Model of Ischemia/Reperfusion and Inflammation of the Kidneys of the Rat.- In Vivo Models of Inflammation.- Wound Healing.- Pharmacodynamic Endpoints in Experimental Models and In Clinical Studies in Humans.- An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans.- A Reply to "An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans".- Quantifying Inflammation In Vivo Using Radiolabeled Antibodies and Leukocytes.- Immunoperoxidase Histochemistry for the Detection of Cellular Adhesion Molecule, Cytokine, and Chemokine Expression in the Arthritic Synovium.- Roles of Nitric Oxide and Superoxide in Inflammation.- Analysis of Nitrite and Nitrate in the Study of Inflammation.- In Vivo Assays for COX-2.- Measurement of 8-epi-PGF2? as a Marker of Lipid Peroxidation In Vivo by Immunoaffinity Extraction and Gas Chromatography-Mass Spectrometry.- Laboratory Assessment of the Acute Phase Response.- Assays of Matrix Metalloproteinases (MMPs) and MMP Inhibitors.
Inhaltsverzeichnis
Part I. In Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory Drugs
Key Stages in the Acute Inflammatory Response and Their Relevance as Therapeutic Targets: Introduction to Part 1
Paul G. Winyard
IkB Kinase and NF-kB Signaling in Response to Pro-Inflammatory Cytokines
Mireille Delhase
Screening for Inhibitors of Tranillegalscription Factors, Using Luciferase Reporter Gene Expression in Transfected Cells
Deborah Phippard and Anthony M. Manning
Adhesion Molecule Expression on Cytokine-Stimulated Human Endothelial Cells
Susan L. Cuvelier and Kamala D. Patel
Phagocytosis by Inflammatory Phagocytes: Experimental Strategies for Stimulation and Quantification
M. Rachel Morris, Sharon Dewitt, Iraj Laffafian, and Maurice B. Hallett
Cytosolic Ca2+ Measurement and Imaging in Inflammatory Cells
Sharon Dewitt, Iraj Laffafian, M. Rachel Morris, and Maurice B. Hallett
Detection and Visualization of Oxidase Activity in Phagocytes
Maurice B. Hallett, Caroline Cole, and Sharon Dewitt
Measurement of Complement Activation
Tom Eirik Mollnes
Measurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using Zymography
Linda Troeberg and Hideaki Nagase
Measurement of Aggrecanase-Generated Interglobular Domain Catabolites in the Medium and Extracts of Cartilage Explants Using Western Blot Analysis
Clare E. Hughes, Christopher B. Little, and Bruce Caterson
In Vitro Model of Human Articular Cartilage Degradation
William D. Shingleton
Part II. In Vivo Models of Inflammation
In Vivo Models of Inflammation: Introduction to Part 2
Derek A. Willoughby
Carrageenan-Induced Paw Edema in the Rat and Mouse
Christopher J. Morris
Pleural Models of Inflammation: Immune and Nonimmune
Adrian R. Moore
Models of Acute Inflammation in the Ear
Miklós Gábor
Migration of Specific Leukocyte Subsets in Response to Cytokine or Chemokine Application In Vivo
Mauro Perretti and Stephen J. Getting
Inflammatory Joint Disease: Clinical, Histological, and Molecular Parameters of Acute and Chronic Inflammation and Tissue Destruction
Nancy L. McCartney-Francis, James Chan, and Sharon M. Wahl
The Assessment of Inflammation, Cartilage Matrix, and Bone Loss in Experimental Monoarticular Arthritis of the Rat
Michael P. Seed
Collagen-Induced Arthritis
Adrian R. Moore
Air-Pouch Models of Inflammation and Modifications for the Study of Granuloma-Mediated Cartilage Degradation
Paul Colville-Nash and Toby Lawrence
Quantitative Analysis of Angiogenesis Using the Murine Chronic Granulomatous Air Pouch
Chandan A. S. Alam
Models of Coronary Artery Occlusion and Reperfusion for the Discovery of Novel Antiischemic and Antiinflammatory Drugs for the Heart
Nicole S. Wayman, Michelle C. McDonald, Prabal K. Chatterjee, and Christoph Thiemermann
Assessment of Anticolitic Drugs in the Trinitrobenzene Sulfonic Acid (TNBS) Rat Model of Inflammatory Bowel Disease
Brendan J. R. Whittle, Maryan Cavicchi, and Dominique Lamarque
An In Vivo Model of Ischemia/Reperfusion and Inflammation of the Kidneys of the Rat
Prabal K. Chatterjee and Christoph Thiemermann
In Vivo Models of Inflammation: Immune Rejection and Skin Transplantation In Vivo
Isabelle Binet and Kathryn J. Wood
Wound Healing: A Model of Dermal Wound Repair
Annette Tomlinson and Mark W. J. Ferguson
Part III. Pharmacodynamic Endpoints in Experimental Models and in Clinical Studies in Humans
An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans: Introduction to Part 3
David R. Blake and Gordon J.
Includes supplementary material: sn.pub/extras
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