Volume 2 specifically:
Provides an essential and comprehensive source for every investigator whose research involves any one aspect of lipids as essential components of biological membranes
Serves as a comprehensive compilation of cutting-edge methodologies centered around lipids as the essential components of biological membranes
With two volumes totaling 72 chapters, presents the first ever published comprehensive reference for the application of liposomes as pharmaceutical nanoscale drug delivery systems and as models for biological membranes
Provides in a very comprehensive way detailed guidance for the utilization of liposomes in Pharmacy, Chemistry, Biochemistry, Biophysics, and Molecular Biology
Offers a truly interdisciplinary approach to make the versatile features of liposomes accessible to a very wide range of investigators in Academia and Industry
Acts as a comprehensive guide to preparing, purifying, and physico-chemically characterizing all currently known types of liposomes
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that "the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer” [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.
1. Utilization of Liposomes for Studying Drug Transfer and UptakeAlfred Fahr and Xiangli Liu2. The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer VesiclesJames R. Reed3. Use of Liposomes to Study Cellular OsmosensorsReinhard Krämer, Sascha Nicklisch, and Vera Ott4. Studying Mechanosensitive Ion Channels Using LiposomesBoris Martinac, Paul R. Rohde, Andrew R. Battle, Evgeny Petrov, Prithwish Pal, Alexander Fook Weng Foo, Valeria Vásquez, Thuan Huynh, and Anna Kloda5. Studying Amino Acid Transport Using LiposomesCesare Indiveri6. Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular MembranesChris Höfer, Andreas Herrmann, and Peter Müller7. Liposomal Reconstitution of Monotopic Integral Membrane ProteinsZahra MirAfzali and David L. DeWitt8. The Reconstitution of Actin Polymerization on LiposomesMark Stamnes and Weidong Xu9. Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains Under Physiological Ionic-Strength ConditionsL.-Ruth Montes, Hasna Ahyayauch, Maitane Ibarguren, Jesus Sot, Alicia Alonso, Luis A. Bagatolli, and Felix M. Goñi10. Visualization of Lipid Domain Specific Protein Sorting in Giant Unilamellar VesiclesMartin Stöckl, Jörg Nikolaus, and Andreas Herrmann11. Biosynthesis of Proteins Inside LiposomesPasquale Stano, Yutetsu Kuruma, Tereza Pereira de Souza, and Pier Luigi Luisi12. Study of Respiratory Cytochromes in LiposomesIseli L. Nantes, Cintia Kawai, Felipe S. Pessoto, and Katia C.U. Mugnol13. Use of Liposomes to Evaluate the Role of Membrane Interactions onAntioxidant ActivitySalette Reis, Marlene Lúcio, Marcela Segundo, and José L.F.C. Lima14. Studying Colloidal Aggregation Using LiposomesJuan Sabín, Gerardo Prieto, Juan M. Ruso, and Félix Sarmiento15. Assessment of Liposome-Cell InteractionsJan A.A.M. Kamps16. Methods to Monitor Liposome Fusion, Permeability, and Interaction with CellsNejat Düzgünes, Henrique Faneca, and Maria C. Pedroso de Lima17. The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in LiposomesDavid Miller and Paula J. Booth18. Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin LabelingWitold K. Subczynski, Marija Raguz, and Justyna Widomska19. Membrane Translocation Assayed by Fluorescence SpectroscopyJana Broecker and Sandro Keller20. Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance SpectroscopyHugo Rubén Arias21. Environmental Scanning Electron Microscope Imaging of Vesicle SystemsYvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U.R. Mohammed, Sarah E. McNeil, and Anil Vangala22. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution ScaleBrigitte Papahadjopoulos-Sternberg23. Atomic Force Microscopy for the Characterization of ProteoliposomesJohannes Sitterberg, Maria Manuela Gaspar, Carsten Ehrhardt, and Udo Bakowsky24. Method of Simultaneous Analysis of Liposome Components Using HPTLC/FIDSophia Hatziantoniou and Costas Demetzos25. Viscometric Analysis of DNA-Lipid ComplexesSadao Hirota and Nejat Düzgünes26. Fluorometric Analysis of Individual Cationic Lipid-DNA ComplexesEdwin
With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 2: Biological Membrane Models focuses on detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies. As a volume in the highly successful Methods in Molecular Biology™ series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.
From the reviews:
"Provide a comprehensive guide to the preparation, characterisation, and potential use of currently known types of liposomes. They are written using an interdisciplinary approach, and so provide essential knowledge of liposomes, for not only researchers in this particular field, but also scientists in wider pharmaceutical and biological areas. ... very inspiring – the potential for liposomes is immense – and these well-written chapters ignited notions of further applications – this is clearly a very exciting field.” (Trudy L. Knight, BTS Newsletter, Issue 37, Winter, 2010)
This volume explores cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. It focuses on methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena.
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that "the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer" [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.
Utilization of Liposomes for Studying Drug Transfer and Uptake.- The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer Vesicles.- Use of Liposomes to Study Cellular Osmosensors.- Studying Mechanosensitive Ion Channels Using Liposomes.- Studying Amino Acid Transport Using Liposomes.- Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes.- Liposomal Reconstitution of Monotopic Integral Membrane Proteins.- The Reconstitution of Actin Polymerization on Liposomes.- Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions.- Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles.- Biosynthesis of Proteins Inside Liposomes.- Study of Respiratory Cytochromes in Liposomes.- Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity.- Studying Colloidal Aggregation Using Liposomes.- Assessment of Liposome-Cell Interactions.- Methods to Monitor Liposome Fusion, Permeability, and Interaction with Cells.- The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes.- Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling.- Membrane Translocation Assayed by Fluorescence Spectroscopy.- Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance Spectroscopy.- Environmental Scanning Electron Microscope Imaging of Vesicle Systems.- Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale.- Atomic ForceMicroscopy for the Characterization of Proteoliposomes.- Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID.- Viscometric Analysis of DNA-Lipid Complexes.- Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes.- Fluorescence Resonance Energy Transfer-Based Analysis of Lipoplexes.- Analysis of Lipoplex Structure and Lipid Phase Changes.- Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery.- FRET Imaging of Cells Transfected with siRNA/Liposome Complexes.- Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes.- Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Pre-formed Liposomes for Diagnostic Imaging and Radionuclide Therapy.- Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant Unilamellar Vesicles.- Liposome Biodistribution via Europium Complexes.- Biosensor-Based Evaluation of Liposomal Binding Behavior.- Use of Liposomes to Study Vesicular Transport.
From the reviews:
"Provide a comprehensive guide to the preparation, characterisation, and potential use of currently known types of liposomes. They are written using an interdisciplinary approach, and so provide essential knowledge of liposomes, for not only researchers in this particular field, but also scientists in wider pharmaceutical and biological areas. ... very inspiring - the potential for liposomes is immense - and these well-written chapters ignited notions of further applications - this is clearly a very exciting field." (Trudy L. Knight, BTS Newsletter, Issue 37, Winter, 2010)
Inhaltsverzeichnis
Utilization of Liposomes for Studying Drug Transfer and Uptake.- The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer Vesicles.- Use of Liposomes to Study Cellular Osmosensors.- Studying Mechanosensitive Ion Channels Using Liposomes.- Studying Amino Acid Transport Using Liposomes.- Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular Membranes.- Liposomal Reconstitution of Monotopic Integral Membrane Proteins.- The Reconstitution of Actin Polymerization on Liposomes.- Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions.- Visualization of Lipid Domain-Specific Protein Sorting in Giant Unilamellar Vesicles.- Biosynthesis of Proteins Inside Liposomes.- Study of Respiratory Cytochromes in Liposomes.- Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant Activity.- Studying Colloidal Aggregation Using Liposomes.- Assessment of Liposome-Cell Interactions.- Methods to Monitor Liposome Fusion, Permeability, and Interaction with Cells.- The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes.- Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin Labeling.- Membrane Translocation Assayed by Fluorescence Spectroscopy.- Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance Spectroscopy.- Environmental Scanning Electron Microscope Imaging of Vesicle Systems.- Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale.- Atomic ForceMicroscopy for the Characterization of Proteoliposomes.- Method of Simultaneous Analysis of Liposome Components Using HPTLC/FID.- Viscometric Analysis of DNA-Lipid Complexes.- Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes.- Fluorescence Resonance Energy Transfer-Based Analysis of Lipoplexes.- Analysis of Lipoplex Structure and Lipid Phase Changes.- Fluorescence Methods for Evaluating Lipoplex-Mediated Gene Delivery.- FRET Imaging of Cells Transfected with siRNA/Liposome Complexes.- Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of Lipoplexes.- Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Pre-formed Liposomes for Diagnostic Imaging and Radionuclide Therapy.- Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant Unilamellar Vesicles.- Liposome Biodistribution via Europium Complexes.- Biosensor-Based Evaluation of Liposomal Binding Behavior.- Use of Liposomes to Study Vesicular Transport.
Klappentext
Efforts to describe and model the molecular structure of biological membranes go back to the beginning of the last century. In 1917, Langmuir described membranes as a layer of lipids one molecule thick [1]. Eight years later, Gorter and Grendel concluded from their studies that ¿the phospholipid molecules that formed the cell membrane were arranged in two layers to form a lipid bilayer¿ [2]. Danielli and Robertson proposed, in 1935, a model in which the bilayer of lipids is sequestered between two monolayers of unfolded proteins [3], and the currently still accepted fuid mosaic model was proposed by Singer and Nicolson in 1972 [4]. Among those landmarks of biomembrane history, a serendipitous observation made by Alex Bangham during the early 1960s deserves undoubtedly a special place. His fnding that exposure of dry phospholipids to an excess of water gives rise to lamellar structures [5] has opened versatile experimental access to studying the biophysics and biochemistry of biological phospholipid membranes. Although during the following 4 decades biological membrane models have grown in complexity and functionality [6], liposomes are, besides supported bilayers, membrane nanodiscs, and hybrid membranes, still an indisputably important tool for membrane b- physicists and biochemists. In vol. II of this book, the reader will fnd detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies.
Volume 2 specifically:
Provides an essential and comprehensive source for every investigator whose research involves any one aspect of lipids as essential components of biological membranes
Serves as a comprehensive compilation of cutting-edge methodologies centered around lipids as the essential components of biological membranes
With two volumes totaling 72 chapters, presents the first ever published comprehensive reference for the application of liposomes as pharmaceutical nanoscale drug delivery systems and as models for biological membranes
Provides in a very comprehensive way detailed guidance for the utilization of liposomes in Pharmacy, Chemistry, Biochemistry, Biophysics, and Molecular Biology
Offers a truly interdisciplinary approach to make the versatile features of liposomes accessible to a very wide range of investigators in Academia and Industry
Acts as a comprehensive guide to preparing, purifying, and physico-chemically characterizing all currently known types of liposomes
Includes supplementary material: sn.pub/extras
