Providing crucial information for the expanding ornamental plant industry, leading researchers in the field compile comprehensive and step-wise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants.
While ornamental plants are produced mainly for their aesthetic value, the propagation and improvement of quality attributes such as leaf types, flower colour and fragrance, longevity and form, plant shape and architecture, and the creation of novel variation are important economic goals for the expanding ornamental industry. In Protocols for In Vitro Propagation of Ornamental Plants , leading researchers in the field compile step-wise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants. Divided into two sections, Section A contains chapters mainly on micropropagation of cut and pot flowers with detailed protocols involving in vitro culture-explants, medium preparation, detailed medium table, shoot initiation and proliferation, root induction, in vitro plant hardening, and field transfer, and Section B delves into reviews on topics such as in vitro production of sweet peas, the status of transgenics in ornamental plants, in vitro conservation, the status of floriculture in Europe, azalea phylogeny, and thin cell layers. As part of the highly successful Methods in Molecular Biology (TM) series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and clear, Protocols for In Vitro Propagation of Ornamental Plants presents key techniques that will be of great use to floriculturists, researchers, commercial companies, biotechnologists, and students dealing with ornamental plants.
Section A: 1. Micropropagation and Organogenesis of Anthurium andreanum Lind cv. Rubrun Oropeza Maira, Mejías Alexander, and Teresa Edith Vargas 2. A Highly Efficient Protocol for Micropropagation of Begonia tuberous Duong Tan Nhut, Nguyen Thanh Hai, and Mai Xuan Phan 3. Micropropagation of Vanda teres (Roxb.) Lindle M. Firoz Alam, Pinaki Sinha, and M. Lokman Hakim 4. In Vitro Propagation Through Axillary Shoot Culture of Ranunculus asiaticus L. Margherita Beruto 5. Micropropagation of Crataeva adansonii D.C. Prodr: An Ornamental Avenue Tree Purnima Tyagi, P.K. Sharma, and S.L. Kothari 6. Strategies for the Micropropagation of Bromeliads Miguel Pedro Guerra and Lirio Luiz Dal Vesco 7. Micropropagation of Poinsettia by Organogenesis Marcos Castellanos, J. Brian Power, and Michael R. Davey 8. Micropropagation of Phalaenopsis Blume Pinaki Sinha, M. Firoz Alam, and M. Lokman Hakim 9. Genetic Transformation of Carnation (Dianthus caryophylus L.) Chalermsri Nontaswatsri and Seiichi Fukai 10. Matrix Supported Liquid Culture and Machine Vision Analysis of Regenerated Shoots of Gladiolus S. Dutta Gupta and V.S. S. Prasad 11. In Vitro Propagation of Carnation (Dianthus caryophyllus L.) Jose L. Casas, Enrique Olmos, and Abel Piqueras 12. In Vitro Propagation of Jasminum officinale L.: A Woody Ornamental Vine Yielding Aromatic Oil from Flowers Sabita Bhattacharya and Sanghamitra Bhattacharyya 13. Micropropagation of Lysionotus pauciflorus Maxim. (Gesneriaceae) Toshinari Godo, Yuanxue Lu, and Masahiro Mii 14. Micropropagation of Rhododendron Tom Eeckhaut, Kristien Janssens, Ellen De Keyser, and Jan DeRiek 15. In Vitro Regeneration and Multiplication of Passiflora hybrid 'Guglielmo Betto' Luca Pipino, Luca Braglia, Annalisa Giovannini, Giancarlo Fascella, and Antonio Mercuri 16. In Vitro Propagation of Rose Pratap Kumar Pati, Navtej Kaur, Madhu Sharma, and Paramvir Singh Ahuja 17. In Vitro Propagation of Chrysanthemum Diana Nencheva 18. Micropropagation of Codiaeum variegatum (L.) Blume and Regeneration Induction via Adventitous Buds and Somatic Embryogenesis Silvia Radice 19. Methods for in vitro Propagation of Pelargonium x hortorum and Others: From Meristems to Protoplasts Noëlle Dorion, Hatem Ben Jouira, Anthony Gallard, Anber Hassanein, Mazen Nassour, and Agnès Grapin 20. Marigold Regeneration and Molecular Analysis of Carotenogenic Genes Alma Angélica Del Villar-Martínez, Pablo Emilio Vanegas-Espinoza, and Octavio Paredes-López 21. Somatic Embryogenesis in in vitro Culture of Leucojum vernum L. Agata Ptak 22. In Vitro Propagation of Australian Native Ornamental Plant, Scaevola Chui Eng Wong and Prem L. Bhalla 23. Micropropagation of Tulip: Production of Virus-free Stock Plants Malgorzata Podwyszynska and Dariusz Sochacki 24. In Vitro Propagation of Ornamental Myrtus (Myrtus communis) Barbara Ruffoni, Carlo Mascarello, and Marco Savona 25. Sunflower Propagation M. L. Mayor, G. Nestares, T. Vega, R. Zorzoli, and L. A. Picardi 26. Clonal Propagation of Cyclamen persicum via Somatic Embryogenesis Traud Winkelmann Section B: 27. In Vitro Production of Sweet Peas (Lathyrus odoratus L.) Via Axillary Shoots Sergio J. Ochatt, C. Conreux, and L. Jacas 28. In vitro Conservation and
It was around 1970, I had just completed a 5-year breeding project aiming at fxing fower colour in gerbera progenies: white, yellow, pink, and red; colour homogeneity was sound, but size and shape still required some improvement. The problem was defnitely resolved by Murashige and Skoog, USA who published a reliable protocol for gerbera micropro- gation. In short, my gerbera seed lines were immediately rendered obsolete by this e- cient cloning system, able to produce millions of plants of a matchless and previously unknown homogeneity, the uniformity of fower shape and colour being the basic requi- ments for the market. The success of micropropagation resulted in a tremendous growth in gerbera fower production worldwide, and this species conquered a leading place in the foriculture industry. This personal experience stresses the impact of micropropagation on the genetic improvement research strategies in ornamentals. Micropropagation has become "in- sive", especially in ornamental plant material issues. Today, hundreds of protocols exist; however, only a modest percentage of them are exploited economically. Thus, only micropropagation of plants with a high market price range, like orchids for instance, has proved cost-effective and achieved great success. Micropropagation is a labour-intensive system: hand-power is estimated to rep- sent 60-70% of total costs. This explains the outsourcing of the major labs in developing countries where labour is cheaper. Nevertheless, certain industrial protocols remain a proprietary technology of leading labs, mostly western, with the exception of Japan and Taiwan.
Provides easily comprehensible reviews and step-by-step in vitro protocols of economically important ornamental plants