Provides cutting edge methods for in vitro and in vivo studies of nucleic acid based drugs
Presents detailed protocols for preparation of non-viral drug delivery vehicles and artificial membrane models
Includes a comprehensive deillegalscription of retrieval of internalizing antibodies from phage display libraries
Gives step-by-step methods for in vitro and in vivo studies of macromolecular delivery across biological barriers
Macromolecular Drug Delivery Dr. Mattias Belting, ed. Table of ContentsPrefaceContributorsDevelopments in Macromolecular Drug Delivery; Review ChapterMattias Belting and Anders WittrupSynthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA Based Cancer Therapies; Review chapterJohn S. Vorhies and John J. Nemunaitis siRNA and DNA Transfer to Cultured CellsBagavathi Gopalakrishnan and Jon WolffPoly(ß-amino esters): Procedures for Synthesis and Gene DeliveryJordan J. Green, Gregory T. Zugates, Robert Langer, and Daniel G. AndersonSystemic Delivery and Preclinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAsChuanbo Zhang, Joseph T. Newsome, Rajshree Mewani, Jin Pei, Prafulla C. Gokhale, and Usha N. KasidPeptide-based Delivery of Steric-block PNA OligonucleotidesSaïd Abes, Gabriela D. Ivanova, Rachida Abes, Andrey A. Arzumanov, Donna Williams, David Owen, Bernard Lebleu and Michael J. GaitCharacterizing Peptide Mediated DNA Internalization in Human Cancer CellsAnders Wittrup and Mattias BeltingSelection and Characterization of Antibodies from Phage Display Libraries against Internalizing Membrane AntigensJohan Fransson and Carl A. K. BorrebaeckArtificial Membrane Models for the Study of Macromolecular DeliveryLena Mäler and Astrid GräslundEnhanced Delivery of Macromolecules into Cells by ElectroendocytosisAlexander Barbul, Yulia Antov, Yosef Rosenberg and Rafi Korenstein In Vitro Systems for Studying Epithelial Transport of MacromoleculesNicole Daum, Andrea Neumeyer, BirgitWahl, Michael Bur, Claus-Michael LehrPreparation of Macromolecule-containing Dry Powders for Pulmonary DeliveryKelly S. Kraft and Marshall GrantMacromolecular Delivery Across the Blood-Brain BarrierKullervo HynynenPositron Emission Tomography (PET) and Macromolecular Delivery In Vivo Ludwig G. Strauss and Antonia Dimitrakopoulou-Strauss
Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines. Ultimately, this cooperation will lead to the use of macromolecular drugs as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat diseases.
From the reviews:
"This volume ... provides details and discussions on contemporary methods available to address the limitations in the delivery of these agents. ... This comprehensive review will be of interest to scientists investigating either the in vitro or in vivo actions of macromolecule agents. Experts in biophysics, chemistry, cell biology, pharmaceutics, and/or preclinical and clinical dug discovery studies will find this book of value. Researchers involved in target therapy strategies for treatment of diseases will want a copy of this book." (Thomas L. Pazdernik, Doody´s Review Service, 2009)
"The title of the book will definitely attract readers of various backgrounds. It seems to indicate that the book covers the delivery of various kinds of macromolecular drugs. ... This will come as a surprise for experts working with other types of macromolecular therapeutics ... . the in vitro and in vivo models described in the book would be of interest to a broad scientific audience. ... the book definitely contains a lot of valuable information for experimental researchers.” (Michal Pechar, ChemMedChem, Issue 4, 2009)
Macromolecular drugs could become new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in a volume which gives a wide-ranging overview of contemporary methods used in the field.
Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines. Ultimately, this cooperation will lead to the use of macromolecular drugs as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat diseases.
Developments in Macromolecular Drug Delivery.- Synthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA-Based Cancer Therapies.- siRNA and DNA Transfer to Cultured Cells.- Poly(?-amino esters): Procedures for Synthesis and Gene Delivery.- Systemic Delivery and Pre-clinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAs.- Peptide-Based Delivery of Steric-Block PNA Oligonucleotides.- Characterizing Peptide-Mediated DNA Internalization in Human Cancer Cells.- Selection and Characterization of Antibodies from Phage Display Libraries Against Internalizing Membrane Antigens.- Artificial Membrane Models for the Study of Macromolecular Delivery.- Enhanced Delivery of Macromolecules into Cells by Electroendocytosis.- In Vitro Systems for Studying Epithelial Transport of Macromolecules.- Preparation of Macromolecule-Containing Dry Powders for Pulmonary Delivery.- Macromolecular Delivery Across the Blood-Brain Barrier.- Positron Emission Tomography (PET) and Macromolecular Delivery In Vivo.
From the reviews:
"This volume ... provides details and discussions on contemporary methods available to address the limitations in the delivery of these agents. ... This comprehensive review will be of interest to scientists investigating either the in vitro or in vivo actions of macromolecule agents. Experts in biophysics, chemistry, cell biology, pharmaceutics, and/or preclinical and clinical dug discovery studies will find this book of value. Researchers involved in target therapy strategies for treatment of diseases will want a copy of this book." (Thomas L. Pazdernik, Doody's Review Service, 2009)
"The title of the book will definitely attract readers of various backgrounds. It seems to indicate that the book covers the delivery of various kinds of macromolecular drugs. ... This will come as a surprise for experts working with other types of macromolecular therapeutics ... . the in vitro and in vivo models described in the book would be of interest to a broad scientific audience. ... the book definitely contains a lot of valuable information for experimental researchers." (Michal Pechar, ChemMedChem, Issue 4, 2009)
Inhaltsverzeichnis
Macromolecular Drug Delivery
Dr. Mattias Belting, ed.
Table of Contents
Preface
Contributors
Developments in Macromolecular Drug Delivery; Review Chapter
Mattias Belting and Anders Wittrup
Synthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA Based Cancer Therapies; Review chapter
John S. Vorhies and John J. Nemunaitis
siRNA and DNA Transfer to Cultured Cells
Bagavathi Gopalakrishnan and Jon Wolff
Poly(ß-amino esters): Procedures for Synthesis and Gene Delivery
Jordan J. Green, Gregory T. Zugates, Robert Langer, and Daniel G. Anderson
Systemic Delivery and Preclinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAs
Chuanbo Zhang, Joseph T. Newsome, Rajshree Mewani, Jin Pei, Prafulla C. Gokhale, and Usha N. Kasid
Peptide-based Delivery of Steric-block PNA Oligonucleotides
Saïd Abes, Gabriela D. Ivanova, Rachida Abes, Andrey A. Arzumanov, Donna Williams, David Owen, Bernard Lebleu and Michael J. Gait
Characterizing Peptide Mediated DNA Internalization in Human Cancer Cells
Anders Wittrup and Mattias Belting
Selection and Characterization of Antibodies from Phage Display Libraries against Internalizing Membrane Antigens
Johan Fransson and Carl A. K. Borrebaeck
Artificial Membrane Models for the Study of Macromolecular Delivery
Lena Mäler and Astrid Gräslund
Enhanced Delivery of Macromolecules into Cells by Electroendocytosis
Alexander Barbul, Yulia Antov, Yosef Rosenberg and Rafi Korenstein
In Vitro Systems for Studying Epithelial Transport of Macromolecules
Nicole Daum, Andrea Neumeyer, BirgitWahl, Michael Bur, Claus-Michael Lehr
Preparation of Macromolecule-containing Dry Powders for Pulmonary Delivery
Kelly S. Kraft and Marshall Grant
Macromolecular Delivery Across the Blood-Brain Barrier
Kullervo Hynynen
Positron Emission Tomography (PET) and Macromolecular Delivery In Vivo
Ludwig G. Strauss and Antonia Dimitrakopoulou-Strauss
Klappentext
Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology¿ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.nnComprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines. Ultimately, this cooperation will lead to the use of macromolecular drugs as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat diseases.
Provides cutting edge methods for in vitro and in vivo studies of nucleic acid based drugs
Presents detailed protocols for preparation of non-viral drug delivery vehicles and artificial membrane models
Includes a comprehensive deillegalscription of retrieval of internalizing antibodies from phage display libraries
Gives step-by-step methods for in vitro and in vivo studies of macromolecular delivery across biological barriers
