Microchip Methods in Diagnostics Ursula Bilitewski, ed. Table of contents Preface Contributors 1. DNA-Microarrays: An Introduction to the Technology Ursula Bilitewski 2. Discussion of the Applicability of Microarrays: Profiling of Leukemias Torsten Haferlach, Ulrike Bacher, Alexander Kohlmann, Claudia Haferlach 3. Expression Profiling Using Affymetrix GeneChip Microarrays Herbert Auer, David L. Newsom and Karl Kornacker 4. Genotyping of mutation in the beta-globin gene using DNA microarrays Martin Dufva and Lena Poulsen 5. Antibody-based Microarrays Christer Wingren and Carl A.K. Borrebaeck 6. Application of Protein ArrayTubes to Bacteria, Toxin and Biological Warfare Agent Detection Ralf Ehricht, Karin Adelhelm, Stefan Monecke, and Birgit Huelseweh 7. Detection of known allergen-specific IgE antibodies by immunological methods Michael Weller and Barbara I. Fall 8. Peptide Microarrays for Serum Antibody Diagnostics Frank F. Bier and Heiko Andresen 9. Microchips for Cell based Assays Martin Dufva 10. Bio-cell Chip Fabrication and Applications Honggu Chun, Dong Soon Lee, and Hee Chan Kim 11. Microchip Capillary Electrophoresis Larry J. Kricka, Sam F. Y. Li, Wai S. Law and Elaine T. T. Tay 12. Detection of Enteropathogenic Escherichia Coli by Microchip Capillary Electrophoresis Larry J. Kricka, Wai S. Law, and Sam F. Y. Li
In this new age of science and technology, microchip methods of analysis have developed into an essential tool for biomedical researchers. Microchip Methods in Diagnostics looks at this increasingly miniaturized technology, focusing on DNA microarrays, protein microarrays, and labs-on-chips, and supporting the introduction of new diagnostic methods based on microtechnologies. The work provides a background on the technological aspects of each of these methods, followed by chapters describing the methods with regard to specific applications. Since it uses only those processes which have reached a certain level of maturation, this volume is able to provide approaches that are applicable in practice even by the non-expert. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
An innovative and cutting-edge work, Microchip Methods in Diagnostics strives to bring the technology of microchips even further into the world of diagnostics, predicting incredible benefits for patients in the years to come.
Includes an introduction into each of the microchip technologies: DNA- microarrays, Antibody- and Peptide-based microarrays, cell chips, and labs-on-chips
Examines the advantages of microchip technologies compared to the conventional methods
Considers commercially available microchip systems as well as procedures for personal fabrication of microchips
Includes procedures for sample pretreatment