Part I: In vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns 1. In vitro Expression and Activity Protocols: Introduction to Part I Roderick J. Flower 2. Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites Hiroyasu Inoue and Rieko Nakata 3. Purification of Recombinant Human COX-1 and COX-2 James K. Gierse 4. Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2 Nicholas R. Staten and Beverly A. Reitz 5. Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System John C. Hunter, Natalie M. Myres, and Daniel L. Simmons 6. Peroxidase Active Site Activity Assay Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett 7. Study of Inhibitors of the PGH Synthases for which Potency is Regulated by the Redox State of the Enzymes Olivier Boutaud and John A. Oates 8. Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors Stefan Laufer and Sabine Luik 9. In vitro Cyclooxygenase Activity Assay in Tissue Homogenates Samir S. Ayoub 10. Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site William F. Hood 11. In vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization Mark C. Walker and James K. Gierse Part II: Extraction and Measurement of Prostanoids and Isoprostanes 12. Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II Paola Patrignani 13. Prostanoid Extraction and Measurement Lorenzo Polenzani and Samir S. Ayoub 14. Measurement of 8-iso-prostagnadin F2a in Biological Fluids as a Measure of Lipid Peroxidation Stefania Tacconelli, Marta L. Capone, and Paola Patrignani Part III: In vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease 15. In vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III Derek W. Gilroy, Melanie Stables, and Justine Newson 16. Protocols to Assess the Gastro-Intestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms Brendan J.R. Whittle 17. Cyclooxygenase Enzymes and Their Products in the Carragenaan-Induced Pleurisy in Rats Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed 18. Iloprost-Induced Nociception: Determination of the Site of Anti-Nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception Samir S. Ayoub and Regina M. Botting
Since the discovery of the pharmacological and toxicological importance of inhibiting the cyclooxygenase (COX) enzymes by non-steroidal anti-inflammatory drugs, much research has gone into the development of methods to study the biological functions of COX-1 and COX-2. This book covers vitro and in vivo techniques routinely used in COX research.
Serves as a detailed step-by-step laboratory manual covering the most up-to-date, widely used protocols to study the biology of the cyclooxygenase enzymes
Includes chapters written by some of the pioneers in the cyclooxygenase field
Acts as a reference that no cyclooxygenase or prostanoid research laboratory should be without
An indispensable tool for pharmaceutical companies engaged in the discovery and development of cyclooxygenase inhibitors
Describes molecular and biochemical techniques used to study the purification, cloning, expression, and activity of the cyclooxygenase enzymes