Ann E. Tollefson, Mohan Kuppuswamy, and William S. M. Woldn TABLE OF CONTENTSn Vol. II Topics: Ad proteins and RNA, lifecycle and host interactions, and phylogeneticsn n 1. Analysis of the Efficiency of Adenovirus Transcriptionn Cristina Iftode and S. J. Flintn 2. The Use of In Vitro Transcription to Probe Regulatory Functions of Viral Protein Domainsn Paul M. Loewenstein, Chao-Zhong Song and Maurice Green n 3. Preparation of Soluble Extracts from Adenovirus Infected Cells for Studies of RNA Splicingn Oliver Mühlemann and Göran Akusjärvin 4. In Vitro Methods to Study RNA Interference During an Adenovirus Infectionn Gunnar Andersson, Ning Xu and Göran Akusjärvin 5. Simultaneous Detection of Adenovirus RNA and Cellular Proteins by Fluorescent Labeling In Situn Eileen Bridgen 6. Study of Nucleolar Localization of Adenovirus Core Proteinsn David A. Matthewsn 7. Analysis of Adenovirus Infections in Synchronized Cellsn David A. Ornelles, Robin N. Shepard and Felicia D. Goodrumn 8. Co-Immunoprecipitation of Protein Complexesn Peter Yaciukn 9. Chromatin Immunoprecipitation (ChIP) to Study the Binding of Proteins to the Adenovirus Genome in Vivon Jihong Yang and Patrick Hearingn 10. Assaying Protein-DNA Interactions In Vivo and In Vitro Using Chromatin Immunoprecipitation and Electrophoretic Mobility Shift Assaysn Pilar Perez-Romero and Michael J. Imperialen 11. Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometryn Anuj Gaggar, Dmitry Shayakhmetov, and Andre Liebern 12. The Use of Cell Microinjection for the in vivo Analysis of Viral Transcriptional Regulatory Protein Domainsn Maurice Green, Andrew Thorburn, Robert Kern and Paul M. Loewensteinn 13. Determination of the Transforming Activities of Adenovirus Oncogenesn Michael Nevels and Thomas Dobnern 14. Human Adenovirus Type 12: Crossing Species Barriers toImmortalize the Viral Genomen Walter Doerflern 15. Measurement of NK Cell Lytic Activity of Adenovirus-Infected or Adenovirus-Transformed Cells n John M. Routesn 16. A Flow Cytometric Assay for Analysis of NK Cell-Mediated Cytolysis of Adenovirus-Transformed Cellsn Graham Bottley, Graham P. Cook, and G. Eric Blairn 17. Large Scale Purification and Crystallization of Adenovirus Hexon n John J. Rux and Roger M. Burnettn 18. Synthesis and Assay of Recombinant Adenovirus Protease n Joseph M. Webern 19. Assay for the Adenovirus Proteinase- Purification of the Enzyme and Synthesis of a Fluorogenic Substraten Walter F. Mangel and William J. McGrathn 20. Cofactors of the Adenovirus Proteinase: Measuring Equilibrium Dissociation Constants and Stoichiometries of Binding n Walter F. Mangel and William J. McGrathn 21. Characterization of the Adenovirus Fiber Proteinn Jeffrey A. Engler and Jeong Shin Hongn 22. Phylogenetic Analysis of Adenovirus Sequencesn Balázs Harrach and Mária Benkön 23. Assessment of Genetic Variability Among Subspecies B1 Human Adenoviruses for Molecular Epidemiology Studiesn Adriana E. Kajon and Dean D. Erdman
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research, including Ad vector construction and use, real-time PCR, use of new animal models, and methods for quantification of Ad virus or virus expression/interactions. The protocols presented are written by trendsetting researchers.
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. The protocols in these volumes provide a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.